GEO DataSets

(Submitter supplied) Francisella tularensis is a Gram-negative bacterium that causes a fatal human disease known as tularemia. The Centers for Disease Control have classified F. tularensis as Category A Tier-1 Select Agent. The virulence mechanisms of Francisella are not entirely understood. Francisella possesses very few transcription regulators, and most of these regulate the expression of genes involved in intracellular survival and virulence. more...

Organism:

Francisella tularensis subsp. holarctica LVS

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23586

12 Samples

Download data: XLS

(Submitter supplied) These microarray studies are part of a larger study characterizing a deletion mutant of the putative transcriptional regulator IclR in Francisella tularensis LVS and SchuS4 strains. The microarrays were performed using RNA isolated from wild-type LVS and a LVS iclR deletion mutant after growing in Chamberlain?s defined media pH 6.3 to early mid-log phase. Results suggest that IclR affects expression of several genes after determining statistically significant differences by SAM.

Organism:

Francisella tularensis

Type:

Expression profiling by array

Platform:

GPL5844

6 Samples

Download data: GPR, MEV

(Submitter supplied) The facultative intracellular bacterium Francisella tularensis causes the zoonotic disease tularemia. F. tularensis resides within host macrophages in vivo and this ability is essential for pathogenesis. The transcription factor MglA is required for expression of several Francisella genes that are necessary for replication in macrophages and for virulence in mice. We hypothesized that identification of MglA-regulated genes in the Francisella genome by transcriptional profiling of wild-type and mglA mutant bacteria would lead to the discovery of new virulence factors utilized by F. more...

Organism:

Francisella tularensis

Type:

Expression profiling by array

Platform:

GPL4062

34 Samples

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(Submitter supplied) Comparison of expression profiles of strains of Francisella to identify virulence factors We used custom microarrays to detail the global gene expression of four strains of Francisella (Schu4, LVS, OR960246, U112) and identified distinct classes of differentially expressed genes during this process.

Organism:

Francisella tularensis subsp. holarctica LVS; Francisella tularensis subsp. holarctica OR96246; Francisella tularensis subsp. tularensis SCHU S4; Francisella; Francisella tularensis subsp. novicida U112

Type:

Expression profiling by array

Platform:

GPL20119

12 Samples

Download data: CEL

(Submitter supplied) This study investigated gene expression profile in wild type and nlrp3 deficient mice infected with F. tularensis LVS. RNA-sequencing was performed to determine the transcript levels of genes using total RNA extracted from lungs of wild type C57BL/6 and Nlrp3-/- mice infected with 1 × 104 CFU of F. tularensis LVS on days 3 and 7 post-infection.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21626

12 Samples

Download data: XLS

(Submitter supplied) Francisella tularensis is a category A select agent based on its infectivity and virulence but disease mechanisms in F. tularensis infection remain poorly understood. A murine pulmonary model of infection was therefore employed to characterize the host immune response to F. tularensis infection and to discern differences in responses to infection with the highly virulent Type A F. tularensis strain Schu4 and to the less virulent Type B live vaccine strain (LVS). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10504

108 Samples

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(Submitter supplied) The highly infectious bacterium Francisella tularensis is a facultative intracellular pathogen, whose virulence requires proliferation inside host cells, including macrophages. Although some Francisella determinants of intracellular growth have been identified, much remains to be understood about the pathogenesis of this organism. In particular, how Francisella responds to its intracellular environment could provide clues about its intracellular biology and reveal pathogenic determinants based on their intracellular expression profiles. more...

Organism:

Francisella tularensis; Coxiella burnetii; Rickettsia prowazekii; Brucella suis; Borrelia hermsii; Burkholderia mallei; Francisella tularensis subsp. tularensis SCHU S4

Type:

Expression profiling by array

Platform:

GPL7246

32 Samples

Download data: CEL, CHP

(Submitter supplied) To assess which F. tularensis LVS transcripts are impacted by loss of bS21-2, we generated cells lacking the gene encoding bS21-2 (LVS ∆rpsU2). We then grew wild-type cells containing an empty vector (LVS pF), cells lacking bS21-2 with empty vector (LVS ∆rpsU2 pF), and cells lacking the native bS21-2 but ectopically expressing bS21-2 from a plasmid (LVS ∆rpsU2 pF-rpsU2-V). We isolated RNA from mid-log phase cells and analyzed by RNA-Seq.

Organism:

Francisella tularensis subsp. holarctica LVS

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32555

7 Samples

Download data: TXT

(Submitter supplied) The bacterium, Francisella tularensis (Ft), is one of the most infectious agents known and classified as a category A bioweapon. Ft virulence is controlled by a unique set of transcription regulators, the MglA-SspA heterodimer, PigR, and the stress signal, ppGpp. These factors activate Francisella pathogenicity island (FPI) gene expression, which is required for virulence. MglA-SspA is expressed during infection and constitutively associates with the σ70 associated RNAP holoenzyme (RNAPσ70), indicating that RNAPσ70-(MglA-SspA) is a virulence specific polymerase. more...

Organism:

Francisella tularensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28561

3 Samples

Download data: BW

(Submitter supplied) We used transposon insertion sequencing (Tn-Seq) to identify the genes that are required for in vitro growth and intramacrophage growth of the live vaccine strain of F. tularensis (LVS).

Organism:

Francisella tularensis subsp. holarctica LVS

Type:

Other

Platform:

GPL27588

5 Samples

Download data: WIG