GEO DataSets

(Submitter supplied) Macrophages are a protective replicative niche for Mycobacterium tuberculosis (Mtb) but can kill the infecting bacterium when appropriately activated. To identify mechanisms of clearance, we compared bacterial restriction by human macrophages after treatment with 26 compounds, including some currently in clinical trials for tuberculosis. All-trans-retinoic acid (ATRA), an active metabolite of vitamin A, drove the greatest increase in Mtb control. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21697

20 Samples

Download data: XLSX

(Submitter supplied) Tuberculosis remains a major cause of death from an infectious disease worldwide, yet only 10% of people infected with Mycobacterium tuberculosis develop disease. Defining both necessary and sufficient immunologic determinants of protection remains a great scientific challenge. Analysis of peripheral blood gene expression profiles of active tuberculosis patients has identified correlates of risk for disease or pathogenesis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

32 Samples

Download data: CEL

(Submitter supplied) A role for vitamin A in host defense against Mycobacterium tuberculosis has been suggested through epidemiological and in vitro studies; however, the antimicrobial mechanism is unclear. Here, we demonstrate that vitamin A mediates host defense through regulation of cellular cholesterol content. Comparison of monocytes stimulated with all-trans retinoic acid (ATRA) or 1,25-dihydroxyvitamin D3, the biologically active forms of vitamin A and vitamin D respectively, indicates that ATRA and 1,25D3 induce mechanistically distinct antimicrobial activities. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4860

Platform:

GPL570

12 Samples

Download data: CEL, CHP

Analysis of monocytes stimulated with biologically active forms of vitamin A and vitamin D. Vitamin A plays a role in host defense against Mycobacterium tuberculosis. Results provide insight into molecular mechanisms underlying the antimicrobial activities for vitamin A and vitamin D in monocytes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 agent, 4 individual sets

Platform:

GPL570

Series:

GSE46268

12 Samples

Download data: CEL, CHP

(Submitter supplied) Human serum derived macrophages were infected with Mtb expressing a transcriptional reporter of viability and on day 5, the cells were sorted for RFP+GFP+ macrophages or RFP+GFP- macrophages.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

6 Samples

Download data: CSV

(Submitter supplied) Background: Dissecting the in-vivo host-pathogen interplay is crucial in understanding the molecular mechanisms governing control or progression of the infection. While Dual RNA-seq offers significant advantages over traditional approaches in the analysis of intracellular infections, to date technical challenges have restricted the application of this technology predominantly to tissue culture infection models. more...

Organism:

Mus musculus; Mycobacterium tuberculosis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL22688 GPL19057

19 Samples

Download data: CSV, TSV, TXT

(Submitter supplied) Mycobacterium tuberculosis (Mtb) is a life-threatening pathogen in humans. Bacterial infection of macrophages usually triggers strong innate immune mechanisms, including IL-1 cytokine secretion. The newer member of the IL-1 family, IL-36, was recently shown to be involved in cellular defense against Mtb. To unveil the underlying mechanism of IL-36 induced antibacterial activity, we analyzed its role in the regulation of cholesterol metabolism, together with the involvement of Liver X Receptor (LXR) in this process. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL23572

30 Samples

Download data: TXT

(Submitter supplied) Mycobacterium tuberculosis has specialised its metabolism to reside extra- and intra-cellularly in the human host. Nutrient availability and their concentrations can vary dramatically in these niches. Lactate is abundant during infection and it is an important signalling molecule regulating the immune response. In addition, lactate is abundant in blood, epithelial mucosa and a number of  other relevant compartments in the host. more...

Organism:

Mycobacterium tuberculosis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25317

18 Samples

Download data: TXT

(Submitter supplied) Mycobacterium tuberculosis (Mtb), the cause of the human pulmonary disease tuberculosis (TB), contributes to approximately 1.5 million deaths every year. Prior work has established that lipids serve as the primary carbon and energy source for Mtb in vivo and fulfill major roles in Mtb physiology and pathogenesis. We conducted a high-throughput screen to identify novel inhibitors of Mtb survival in its host macrophage. more...

Organism:

Mycobacterium tuberculosis; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL31944 GPL25317

21 Samples

Download data: TXT

(Submitter supplied) Primary human monocytes were isolated from four healthy human blood donors. Monocytes were isolated from PBMC buffy coats using plastic adherence for 4 hours. Monocytes were allowed to differentiate into macrophages over a period of 1 week. Macrophages from each of the 4 donors were split into two groups - uninfected and infected with Mycobacterium tuberculosis (Mtb). Cells in the infection group were infected with Mtb for 48 hours at a multiplicity of infection (MOI) of 10. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

8 Samples

Download data: CSV

(Submitter supplied) Primary human monocytes were isolated from four healthy donors. Monocytes were differentiated into macrophages, infected with virulent Mycobacterium tuberculosis (Mtb), strain H37Rv, for 24 or 48 hours, at a multiplicity of infection of 5 or 10. Following infection, infected cells and time-matched uninfected controls were harvested, total RNA including small RNAs was isolated and used for next-generation small RNA sequencing. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18573

24 Samples

Download data: CSV

(Submitter supplied) We have previously reported that Mycobacterium tuberculosis Rv2837c (cnpB) encodes a phosphodiesterase that specifically cleaves cyclic di-AMP (c-di-AMP) into AMP. Deletion of cnpB results in significant virulence attenuation in a mouse pulmonary infection model, which is very likely due to the significantly elevated c-di-AMP levels as overexpression of Mtb diadenylate cyclase, disA, also leads to a similar outcome. more...

Organism:

Mycobacterium tuberculosis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18768

6 Samples

Download data: XLSX

(Submitter supplied) The purpose of this study was to determine (i) the interplay between Mycobacterium tuberculosis response to acidic pH and cholesterol, two signals experienced concurrently by the bacterium during host colonization, and (ii) the role of the transcription factor Mce3R in regulation of this response.

Organism:

Mycobacterium tuberculosis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17879

16 Samples

Download data: CSV

(Submitter supplied) Transcriptional response of Mycobacterium tuberculosis ΔlucA::hyg mutant and complemented strains vs. WT in resting murine bone marrow-derived macrophages was analyzed in this study. This data demonstrates that deletion of lucA leads to perturbed expression of the genes linked to fatty acid and cholesterol metabolism of the pathogen during macrophage infection. It supports an overall conclusion of the publication that LucA serves an important role in function of fatty acid and choletserol transporters.

Organism:

Mycobacterium tuberculosis

Type:

Expression profiling by array

Platform:

GPL19382

16 Samples

Download data: TXT