GEO DataSets

(Submitter supplied) Our objective was to determine whether gene expression in Drosophila melanogaster selectively bred for long or short night sleep duration changes detectably across generations. To meet this objective, we performed transcriptional profiling of ten pooled whole adult individuals from four selected populations and two control populations across 13 generations. We quantified differential expression among selection scheme (long sleep, short sleep, or unselected control), generation (generation 0; then generations 2-13), and sex for each gene.

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17275

312 Samples

Download data: CSV

(Submitter supplied) Our primary objective was to characterize the amount of variation in transcript abundance among individual flies with identical genotypes. We also wanted to determine which analysis methods would be optimal for RNA-Seq data. To meet these objectives, we performed transcriptional profiling of whole adult individuals from 16 Drosophila Genetic Reference Panel (DGRP) lines. We quantified differential expression among genotypes, environments, and sexes.

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13304

851 Samples

Download data: FA, GTF, TXT, XLSX

(Submitter supplied) To gain insight into the dynamic molecular processes that are altered during prolonged wakefulness and during sleep. We performed an RNA expression profiling study examining temporal changes in the brain of Drosophila in relationship to the duration of prior sleep or wakefulness. Our experimental design allowed us to determine whether genes identified as differentially regulated between sleep and wakefulness were up- or down-regulated in these states. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Dataset:

GDS1686

Platform:

GPL72

30 Samples

Download data: CEL, EXP

Analysis of brain of Canton-S females deprived of sleep by perturbations during their normal sleep period. Perturbation effect also assessed during their active period to control for its effect during sleep deprivation. Results suggest processes altered during prolonged wakefulness and during sleep.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, transformed count, 6 protocol, 4 time sets

Platform:

GPL72

Series:

GSE4174

30 Samples

Download data: CEL, EXP

(Submitter supplied) We used RNAseq to detect differential gene expression in Drosophila melanogaster males exposed to rival males or not for a period of 2, 26 or 50h. Dahomey wild type males were raised at a density of 100 larvae per vial on SYA medium. Upon eclosion, males were stored 10 per vial and then at 1d old placed in individual vials for 5days. Rival wild type males were then introduced to the focal males for 2, 26 or 50h. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13304

36 Samples

Download data: CSV

(Submitter supplied) An analysis of the transcriptional profiles of weak genetic perturbations generated via P-elements measured as heterozygotes in wing imaginal discs of Drosophila melanogaster. Abstract: A major objective of genomics is to elucidate the mapping between genotypic and phenotypic space as a step toward understanding how small changes in gene function can lead to elaborate phenotypic changes. One approach that has been utilized is to examine overall patterns of co-variation between phenotypic variables of interest, such as morphology, physiology and behavior, and underlying aspects of gene activity, in particular transcript abundance on a genome wide scale. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL7740

213 Samples

Download data: TXT

(Submitter supplied) Genetic background effects contribute to the phenotypic consequences of mutations, and are pervasive across all domains of life that have been examined, yet little is known about how they modify genetic systems. In part this is due to the lack of tractable model systems that have been explicitly developed to study the genetic and evolutionary consequences of background effects. In this study we demonstrate that phenotypic expressivity of the scalloped[E3] mutation of Drosophila melanogaster is background dependent, and is the result of at least one major modifier segregating between two standard lab wild-type strains. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL3830

24 Samples

Download data: TXT

(Submitter supplied) The goal of this study is to identify co-expressed genes downstream of Atonal and Senseless. These gene lists are used as candidate target genes (technically: as foreground sets) in computational predictions of cis-regulatory elements using the cisTargetX method (http://med.kuleuven.be/cme-mg/lng/cisTargetX). Together, the gene expression results and cis-regulatory predictions, yield a gene regulatory network underlying the early events in retinal differentiation. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL1322

20 Samples

Download data: CEL

(Submitter supplied) Alcoholism is a complex disorder determined by interactions between genetic and environmental risk factors. Drosophila represents a powerful model system to dissect the genetic architecture of alcohol sensitivity, as large numbers of flies can readily be reared in defined genetic backgrounds and under controlled environmental conditions. Furthermore, flies exposed to ethanol undergo physiological and behavioral changes that resemble human alcohol intoxication, including loss of postural control, sedation, and development of tolerance. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Dataset:

GDS3072

Platform:

GPL1322

24 Samples

Download data: CEL, CHP

Analysis of alcohol resistant and sensitive strains. Strains generated by artificial selection for alcohol sensitivity over multiple generations. Results provide insight into the molecular basis of alcohol sensitivity.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, count, 2 gender, 2 other, 3 strain sets

Platform:

GPL1322

Series:

GSE7614

24 Samples

Download data: CEL, CHP

(Submitter supplied) Using RNA interference, we dorsalized and ventralized Nasonia embryos. By comparing transcriptomes from the RNAi cases to each other and to controls, we identify >100 genes expressed along the embryonic DV axis

Organism:

Nasonia vitripennis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16488

6 Samples

Download data: FA, FPKM_TRACKING, TXT

(Submitter supplied) The metronome-like circadian regulation of sleep timing must still adapt to an uncertain environment. Recent studies in Drosophila indicate that neuromodulation not only plays a key role in clock neuron synchronization but also affects interactions between the clock network and brain sleep centers. We show here that the targets of neuromodulators, G-Protein Coupled Receptors (GPCRs), are highly enriched in the fly brain circadian clock network. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19132

8 Samples

Download data: TXT

(Submitter supplied) As opposed to syndromic CNVs caused by single genes, extensive phenotypic heterogeneity in variably-expressive CNVs complicates disease gene discovery and functional evaluation. Here, we propose a complex interaction model for pathogenicity of the autism-associated 16p11.2 deletion, where CNV genes interact with each other in conserved pathways to modulate expression of the phenotype. Using multiple quantitative methods in Drosophila RNAi lines, we identify a range of neurodevelopmental phenotypes for knockdown of individual 16p11.2 homologs in different tissues. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13304

21 Samples

Download data: TXT

(Submitter supplied) Food consumption is critical for animal survival and reproduction. The biomedical and economic consequences of metabolic diseases arising from excessive food intake, however, are a burden for human society. While the role of neuroendocrine feedback loops, food sensing modalities, and physiological state in regulating food intake are increasingly well understood, other genetic mechanisms remain elusive. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17275

32 Samples

Download data: TXT