GEO DataSets

(Submitter supplied) Few studies have investigated host-bacterial interactions at sites of infection in humans using transcriptomics and metabolomics. Haemophilus ducreyi causes cutaneous ulcers in children and the genital ulcer disease chancroid in adults. We developed a human challenge model in which healthy adult volunteers are infected with H. ducreyi on the upper arm until they develop pustules. Here, we characterized host-pathogen interactions in pustules using transcriptomics and metabolomics and examined interactions between the host transcriptome and metabolome using integrated omics. more...

Organism:

Homo sapiens; [Haemophilus] ducreyi

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL31188 GPL32713 GPL24676

20 Samples

Download data: TXT

(Submitter supplied) A major gap in understanding infectious diseases is the lack of information about molecular interaction networks between pathogens and the human host. Haemophilus ducreyi causes the genital ulcer disease chancroid in adults and is a leading cause of cutaneous ulcers in children in the tropics. We developed a model in which human volunteers are infected on the upper arm with H. ducreyi until they develop pustules. more...

Organism:

[Haemophilus] ducreyi; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20301 GPL26639 GPL26640

12 Samples

Download data: TXT

(Submitter supplied) To better understand the molecular mechanisms underlying Haemophilus ducreyi infection in humans, here we determined the transcriptional profile of H. ducreyi in human lesions using RNA-Seq and compared it to that of in vitro growth. We were able to show that the in vivo transcriptome did not resemble that of in vitro growth. Compared to the inoculum, H. ducreyi harvested from pustules differentially expressed ~120 genes, of which 68 were upregulated. more...

Organism:

[Haemophilus] ducreyi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21166

4 Samples

Download data: XLSX

(Submitter supplied) The transcriptional response of Haemophilus ducreyi to anaerobiosis was determined over the course of 18 h. Cells were collected from aerobic and anaerobic cultures at 4, 8, or 18 hours .

Organism:

[Haemophilus] ducreyi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31188

24 Samples

Download data: TXT

(Submitter supplied) Comparison of gene expression from subjects who resolved or formed pustules to H.ducreyi. In human inoculation experiments, the cutaneous immune response to Haemophilus ducreyi consists of serum, PMN, macrophages, T cells and myeloid DC. In reinfection experiments, some subjects form pustules twice (PP group) or resolve infection twice (RR group). Although pustule formation is associated with serum resistance and phagocytic failure, there are no differences in the ability of isolated phagocytes or serum obtained from PP and RR subjects to ingest or kill H. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

24 Samples

Download data: CEL

(Submitter supplied) The goal of this study was to compare the global trascription profile of a Haemophilus ducreyi hfq deletion mutant to that of the wild type parental strain.

Organism:

[Haemophilus] ducreyi 35000HP; [Haemophilus] ducreyi

Type:

Expression profiling by array

Platform:

GPL7741

6 Samples

Download data: GPR

(Submitter supplied) To better understand the role of the (p)ppGpp-mediated stringent response in control of H. ducreyi virulence determinants, here we defined genes potentially regulated by either (p)ppGpp or DksA by using RNA-seq. We were able to show that loss of either (p)ppGpp or DksA resulted in dysregulation of multiple genes including several known virulence determinants. We also show that loss of (p)ppGpp or DksA resulted in differential expression of putative H. more...

Organism:

[Haemophilus] ducreyi 35000HP

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL19931

36 Samples

Download data: XLSX

(Submitter supplied) To better understand the role of H. ducreyi CpxRA in controlling virulence determinants, here we defined genes potentially regulated by CpxRA by using RNA-Seq. Activation of CpxR by deletion of cpxA repressed nearly 70% of its targets, including seven established virulence determinants. Inactivation of CpxR by deletion of cpxR differentially regulated few genes and increased the expression of one virulence determinant. more...

Organism:

[Haemophilus] ducreyi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21166

36 Samples

Download data: XLSX

(Submitter supplied) To potentially understand how H. ducreyi responds to membrane stress, here we defined RpoE-dependent genes using RNA-Seq. We identified 180 RpoE-dependent genes, of which 98% were upregulated; a major set of these genes encodes homologues of envelope maintenance and repair factors. We also identified and validated a putative RpoE promoter consensus sequence, which was enriched in the majority of RpoE-dependent targets. more...

Organism:

[Haemophilus] ducreyi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21166

24 Samples

Download data: XLSX