GEO DataSets

(Submitter supplied) Nasal colonization is well described; however, we have limited knowledge about S. aureus throat colonization. The main objective of this project was to explore differentially expressed genes (DEGs) in S. aureus throat isolate TR145 exposed for 1 or 3 hours (h) to human tonsil epithelial cells (HTEpiC) by using RNA sequencing (RNA-seq) and pathway analysis. We have shown the suitability of using HTEpiC as an in vitro model for investigating key determinants in S. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28116

14 Samples

Download data: FASTA, TXT

(Submitter supplied) Better understanding of S. aureus throat colonization in the presence of other competing/coexisting microbes may provide insight into S. aureus adaptation to the human throat and recurrence of infection. In this work, we explore the responses triggered by the encounter between two common throat bacteria, S. aureus and S. anginosus, in the presence of human tonsillar epithelial cells. We performed an in vitro coculture experiment followed by RNA sequencing. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28116

12 Samples

Download data: FASTA, TXT

(Submitter supplied) Purpose: The goal of this study was to identify Staphylococcus aureus genes which are important during establishment of metastatic infections in bloodstream infections. Methods: Pooled mariner transposon mutant libraries were generated as previously described (PMID: 27185949). The libraries were sequenced on the Illumina HiSeq 2500 platform with the transposon-specific oligonucleotide primer Himar1-Seq. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19006

12 Samples

Download data: CSV

(Submitter supplied) Staphylococcus aureus is an opportunistic pathogen that colonizes the anterior nares of about 30-50% of the population. Colonization is most often asymptomatic, however, self-inoculation through inhalation, ingestion, or an open wound, can give rise to potentially fatal infections of the deeper tissues and blood. Like all bacteria, S. aureus is able to sense and respond to environmental cues and modify gene expression to adapt to specific environmental conditions. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25144

9 Samples

Download data: XLSX

(Submitter supplied) Staphylococcus aureus is a gram-positive cocci and an important human commensal bacteria and pathogen. S. aureus infections are increasingly difficult to treat because of the emergence of highly resistant MRSA (Methicillin-resistant S. aureus) strains. Here we present a method to study differential gene expression in S. aureus using high-throughput RNA-sequencing (RNA-seq). We use RNA-seq to examine the differential gene expression in S. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17452

3 Samples

Download data: WIG

(Submitter supplied) The purpose of the study was to investigate the transcriptomic responses of S. aureus and HUVEC cells before and during S. aureus invasion of HUVEC cells.

Organism:

Homo sapiens; Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24676 GPL28575 GPL27158

12 Samples

Download data: TSV

(Submitter supplied) The regulatory effects of H3K27me3 on target genes expressions were analyzed by comparing S. aureus mastitis resistant and susceptible cows. The differentially expressed genes are mainly associated with immune and disease-related processes, which were negatively regulated by H3K27me3 modification on the up 2Kb regions relative to TSS in S. aureus mastitis cattle.

Organism:

Bos taurus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL15749

6 Samples

Download data: TAR

(Submitter supplied) The differential gene expressions of rat mucosa colonized with single or multi-species of MRSA or PA were studied using RNA-sequencing of total transcriptome. In multi-species in-vitro biofilms PA partially inhibited SA growth. However, no significant inhibition of MRSA was detected during in-vivo colonization of multi-species in rat bullae. A total of 1797 genes were significantly (p < 0.05) differentially expressed in MRSA or PA or MRSA+PA colonized rat middle ear mucosa with respect to the control. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18694

4 Samples

Download data: TXT