GEO DataSets

Analysis of fish preovulatory ovaries collected during late vitellogenesis, post-vitellogenesis and oocyte maturation. In fish, the post-vitellogenic period is critical for completion of the oogenetic process. Results provide insight into molecular events occurring during the preovulatory period.

Organism:

Oncorhynchus mykiss

Type:

Expression profiling by array, transformed count, 3 development stage sets

Platform:

GPL3650

Series:

GSE4871

13 Samples

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(Submitter supplied) Nylon microarrays displaying 9152 rainbow trout cDNAs were hybridized using RNA samples originating from ovarian tissue collected during late vitellogenesis, post-vitellogenesis and oocyte maturation. Differentially expressed genes were identified using a statistical analysis. A supervised clustering analysis was performed using only differentially expressed genes in order to identify gene clusters exhibiting similar expression profiles. more...

Organism:

Oncorhynchus mykiss

Type:

Expression profiling by array

Dataset:

GDS3163

Platform:

GPL3650

13 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Oncorhynchus mykiss; Bos taurus; Xenopus laevis; Mus musculus

Type:

Expression profiling by array

4 related Platforms

75 Samples

Download data: TXT

(Submitter supplied) Somatic cells surrounding the oocyte were sampled at the following stages: developmentally incompetent or poorly competent prophase I oocytes (NC1 oocytes), developmentally competent prophase I oocytes (C1 oocytes), and developmentally competent metaphase II oocytes (C2 oocytes). NC1 cumulus cells (CC) were sampled from immature calf oocytes, C1 samples from immature cow oocytes, and C2 samples from in vivo matured cow oocytes.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL9712

17 Samples

Download data: TXT

(Submitter supplied) Cumulus cells surrounding the oocyte were sampled at the following stages: developmentally incompetent or poorly competent prophase I oocytes (NC1 oocytes), developmentally competent prophase I oocytes (C1 oocytes), and developmentally competent metaphase II oocytes (C2 oocytes). NC1 samples were collected from immature, unexpanded cumulus-oocytes complexes (COC) from prepubertal (3-week-old) mice, C1 samples from immature, unexpanded cumulus-oocytes complexes (COC) from adult (8-week-old) and C2 samples from mature, expanded COCs obtained from the oviduct from 8-week-old mice after standard superovulation protocol.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

15 Samples

Download data: TXT

(Submitter supplied) Somatic cells surrounding the oocyte were sampled at the following stages: developmentally incompetent or poorly competent prophase I oocytes (NC1 oocytes), developmentally competent prophase I oocytes (C1 oocytes), and developmentally competent metaphase II oocytes (C2 oocytes). NC1 samples were collected from immature stage IV follicles, C1 samples from immature stage VI follicles, and C2 samples from in vitro matured stage VI follicles.

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL11258

15 Samples

Download data: TXT

(Submitter supplied) Somatic cells surrounding the oocyte were sampled at the following stages: developmentally incompetent or poorly competent prophase I oocytes (NC1 oocytes), developmentally competent prophase I oocytes (C1 oocytes), and developmentally competent metaphase II oocytes (C2 oocytes). NC1 samples were collected from late vitellogenic females (LV), C1 samples from post-vitellogenic females (PV), and C2 samples from females undergoing meiotic maturation (Germinal Vesicle Breakdown)

Organism:

Oncorhynchus mykiss

Type:

Expression profiling by array

Platform:

GPL15258

28 Samples

Download data: TXT

(Submitter supplied) Metabolic processes and sexual maturation closely interact during the long-distance reproductive migration of many fish species to their spawning grounds. In the present study, we have for the first time used exercise experimentally to investigate the effects on sexual maturation in rainbow trout. Pubertal autumn-spawning seawater-raised female rainbow trout Oncorhynchus mykiss (n=26; 50-cm, 1.5-kg) were rested or swum at a near optimal speed of 0.75 body-lengths per second in a 6,000 L swim-flume under natural reproductive conditions (16 °C fresh-water, starvation, 8h-light:16h-dark photoperiod). more...

Organism:

Oncorhynchus mykiss

Type:

Expression profiling by array

Platform:

GPL6154

2 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Oncorhynchus mykiss

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL15840 GPL15841

30 Samples

Download data: TXT

(Submitter supplied) The study aimed to characterize miRNA expression in rainbow trout ovary during ovarian development from immature to mature stages. Whole ovary were collected at the following stages: immature pre-vitelogenesis (IMM), mid-vitellogenesis (MV), late vitellogenesis (LV), post-vitellogenesis (PV) and mature while meiotic maturation is in progress (MAT).

Organism:

Oncorhynchus mykiss

Type:

Non-coding RNA profiling by array

Platform:

GPL15841

14 Samples

Download data: TXT

(Submitter supplied) The study aimed to characterize gene expression in rainbow trout ovary during ovarian development from immature to mature stages. Whole ovary were collected at the following stages: immature pre-vitelogenesis (IMM), mid-vitellogenesis (MV), late vitellogenesis (LV), post-vitellogenesis (PV) and mature while meiotic maturation is in progress (MAT).

Organism:

Oncorhynchus mykiss

Type:

Expression profiling by array

Platform:

GPL15840

16 Samples

Download data: TXT

(Submitter supplied) Gene expression analysis in cc from COCs with different chromatin configuration (GV0-3)

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

12 Samples

Download data: TXT

(Submitter supplied) The present study aimed at studying the rainbow trout egg transcriptome using 9152-cDNA microarrays after natural or controlled ovulation. The analysis of egg transcriptome after natural or controlled ovulation led to the identification of 26 genes. We observed that both hormonal induction and photoperiod control of ovulation induced significant changes in the egg mRNA abundance of specific genes. We demonstrate that hormonal induction of ovulation has an impact on the egg mRNA abundance of specific genes even though the resulting effects on the developmental potential of the egg is so far unknown. more...

Organism:

Oncorhynchus mykiss

Type:

Expression profiling by array

Platform:

GPL3650

28 Samples

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(Submitter supplied) Cumulus-oocyte complexes were isolated a seperate time-points to generate temporal complexes. Targets from two biological replicates at each time point (0h, 8h, 16h post-hCG treatment) were generated and the expression profiles were determined using Affymetrix GeneChip Mouse Genome 430 2.0 Arrays. Comparisons between the sample groups allow the identification of genes with temporal expression patterns. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1677

Platform:

GPL1261

6 Samples

Download data: CEL

Analysis of cumulus oocyte complexes (COCs) isolated from ovaries prior to ovulation or from the oviduct after ovulation. Follicular growth stimulated using equine chorionic gonadotropin (CG) and ovulation induced using human CG. Results identify candidate genes in COCs that impact ovulation.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 agent, 3 time sets

Platform:

GPL1261

Series:

GSE4260

6 Samples

Download data: CEL

(Submitter supplied) Trout spermatogenesis proceeds seasonally in a way that all morphological and cellular events tend to be synchronized. We thus used gonads at key stages of the male reproductive cycle together with isolated germ cell populations to study the changes in gene expression underlying testis development. Statistical analysis followed by clustering of expression profiles allowed the discrimination of sequential events of gene activation or repression during testis development and/or throughout the germline.

Organism:

Oncorhynchus mykiss

Type:

Expression profiling by array

Platform:

GPL3650

38 Samples

Download data: QTF

(Submitter supplied) Abstract. The molecular pathways in embryonic vertebrates leading to gonad formation in each sex are incompletely understood. The purpose of this study was to identify novel genes that could be associated with sex-specific gonadal differentiation in a fish, the rainbow trout (Oncorhynchus mykiss). This study was facilitated by a custom microarray based on 7,681 genes derived from embryonic rainbow trout gonad cDNA libraries and public databases. more...

Organism:

Oncorhynchus mykiss

Type:

Expression profiling by array

Platform:

GPL11151

16 Samples

Download data: CEL

(Submitter supplied) Animals and samplings: Research involving animal experimentation has been approved by the author's institution (authorization number 35-14) and conforms to NIH guidelines. All-male and all-female rainbow trout populations were obtained from the INRA experimental fish farm (Drennec, France) using breeders from the Mirwart strain. At 55 days post-fertilization (55 dpf, i.e., the onset of the free swimming), a male and female batch of 1500 fry each were transferred in 0.3 m3 tanks with recirculating water conditions. more...

Organism:

Oncorhynchus mykiss

Type:

Expression profiling by RT-PCR

Platform:

GPL1793

28 Samples

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(Submitter supplied) Interpretation of toxicogenomic experiments conducted with ovary tissue from asynchronous-spawning small fish species are complicated by background variation in the relative abundance and proportion of follicles at different stages within the ovary tissue sample. This study employed both real-time quantitative PCR and a 15,000 gene oligonucleotide microarray to examine variation in the fathead minnow (Pimephales promelas) ovarian transcriptional profile as a function of quantitative and qualitative differences in ovarian histology. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL9248

30 Samples

Download data: TXT

(Submitter supplied) Comparison of gene expression of exposed versus non-exposed Oncorhynchus mykiss hepatocytes to four model chemicals and a synthetic mixture. Hepatocytes were exposed for 24 hours to a single chemical and a synthetic mixture of 10 nM 17 alpha-ethinylestradiol (EE2), 0.75 nM 2,3,7,8-tetrachloro-di-benzodioxin (TCDD), 100 μM paraquat and 0.75 μM 4-nitroquinoline-1-oxide (NQO). Keywords: Exposed vs. control

Organism:

Salmo salar; Coregonus clupeaformis; Osmerus mordax; Oncorhynchus mykiss; Oncorhynchus tshawytscha

Type:

Expression profiling by array

Platform:

GPL2716

40 Samples

Download data: TXT