GEO DataSets

Analysis of differentiating E14 TG2a ESCs with cell surface expression of 5T4. The upregulation of 5T4 expression is associated with loss of pluripotency in ES cells. Results provide insight into molecular mechanisms underlying the balance between pluripotency and differentiation in ES cells.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 cell type, 2 growth protocol, 2 other sets

Platform:

GPL1261

Series:

GSE20372

6 Samples

Download data: CEL

(Submitter supplied) E14 TG2a cells grown with LIF were disaggregated and FACS sorted for cell surface 5T4 negativity versus E14 TG2a cells grown without LIF for 3 days and sorted for 5T4 positivity.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4207

Platform:

GPL1261

6 Samples

Download data: CEL

(Submitter supplied) Heterogeneity, shortage of material, and lack of progenitor-specific cell surface markers are major obstacles to elucidating the mechanisms underlying developmental processes. Here we report a proteomic platform that alleviates these difficulties and demonstrate its effectiveness in fractionating heterogeneous cultures of early endoderm derived from human embryonic stem cells. The approach, designated cell-capture antibody array, is based on highly parallel, comparative screening of live cell populations using hundreds of antibodies directed against cell-surface antigens. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

5 Samples

Download data: CEL

(Submitter supplied) We examined all transcriptome-level expressions in three initial cell-population densities (862, 1724 and 5172 cells/cm2) in the first two days of differentiation in N2B27. We collected cells in 10-mL tubes and centrifuged them using a pre-cooled centrifuge. We then extracted RNA from each cell-pellet using the PureLink RNA Mini Kit (Ambion, Life Technologies) according to its protocol. We next prepared the cDNA library with the 3′ mRNASeq library preparation kit (Quant-Seq, Lexogen) according to its protocol. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16417

21 Samples

Download data: DIFF, FPKM_TRACKING, TXT, XLSX