GEO DataSets

Analysis of circulating CD4+ and CD8+ T-cells from adult patients with severe or nonsevere asthma. CD4+ T cells contribute to the development and maintenance of an inflammatory response in asthmatic patients. Results provide insight into the role of CD8+ T cells in severe asthma.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 cell type, 3 disease state sets

Platform:

GPL570

Series:

GSE31773

40 Samples

Download data: CEL

(Submitter supplied) Comparison of mRNA expression showed widespread changes in the circulating CD8+ but not CD4+ T-cells from patients with severe asthma. No changes were observed in the CD4+ and CD8+ T-cells in non-severe asthmatics versus healthy controls. Bioinformatics analysis showed that the changes in CD8+ T-cell mRNA expression were associated with multiple pathways involved in T-cell activation. As with mRNAs, we also observed widespread changes in expression of non-coding RNA species including natural antisense, pseudogenes, intronic long ncRNAs and long intergenic long ncRNAs in CD8+ T-cells from severe asthmatics. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4425

Platform:

GPL570

40 Samples

Download data: CEL

(Submitter supplied) The aim was to assess miRNA expression in 3 human ex-vivo CD8+ T cell subsets which span from antigen inexperienced cells (Naïve) to early memory cells (central memory, Tcm) and later stage memory cells (effector memory, Tem) CD8+ T cells were sorted on a FACS Aria II machine. N = naïve = CD8+, CCR7+, CD45RA+, CD45RO-, Tcm = central memory = CD8+, CCR7+, CD45RA-, CD45RO-,Tem= effector memory = CD8+, CCR7-, CD45RA-, CD45RO+

Organism:

Homo sapiens; synthetic construct

Type:

Non-coding RNA profiling by array

Platform:

GPL8786

9 Samples

Download data: CEL

(Submitter supplied) ABSTRACT: OBJECTIVE. Smoking suppresses PD-1 expression in patients with rheumatoid arthritis (RA). In this study, we assess if smoking changed the epigenetic control over CD8+ T cell memory formation through a microRNA (miR) dependent mechanism. METHODS. Phenotypes of CD8+ T cells from smokers and non-smokers, RA and healthy, were analyzed by flow cytometry. A microarray analysis was used to screen for differences in miR expression. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL21263

16 Samples

Download data: TXT

(Submitter supplied) Primary biliary cholangitis (PBC), formally known as primary biliary cirrhosis, is an autoimmune liver disease of unknown pathogenesis. Consequently, therapeutic targets for PBC have yet to be identified. As CD4+ T cells play a pivotal role in immunological dysfunction observed in PBC, we analyzed microRNA(miRNA) and mRNA expression in CD4+ T cells to investigate PBC pathogenesis and identify novel therapeutic targets.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL16770

12 Samples

Download data: TXT

(Submitter supplied) Primary biliary cholangitis (PBC), formally known as primary biliary cirrhosis, is an autoimmune liver disease of unknown pathogenesis. Consequently, therapeutic targets for PBC have yet to be identified. As CD4+ T cells play a pivotal role in immunological dysfunction observed in PBC, we analyzed microRNA(miRNA) and mRNA expression in CD4+ T cells to investigate PBC pathogenesis and identify novel therapeutic targets.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL14550

12 Samples

Download data: TXT

(Submitter supplied) Dysregulated expression of long noncoding RNAs (lncRNAs) has been demonstrated as being implicated in a variety of human diseases. In the study we aimed to determine lncRNA profile in CD8+ T cells response to active tuberculosis (TB).

Organism:

Homo sapiens

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platform:

GPL16956

6 Samples

Download data: TXT

(Submitter supplied) This study describes differential miRNA expression in small intestinal lamina propria leukocyte samples longitudinally during the course of SIV infection of rhesus macaques. Notably, the T-cell activation associated miR-15b, miR-142-3p, miR-142-5p and miR-150 expression was significantly downregulated at 90 and 180DPI. Further, reporter and overexpression assays validated IRAK1 as a direct miR-150 target. more...

Organism:

Homo sapiens; Macaca mulatta

Type:

Expression profiling by RT-PCR

Platform:

GPL17797

25 Samples

Download data: TXT

(Submitter supplied) miRNA profiling of resting and activated T cells

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL10403

1 Sample

Download data: TXT

(Submitter supplied) Background: Asthma has been associated with impaired interferon response. Multiple cell types have been implicated in such response impairment and may be responsible for asthma immunopathology. However, existing models to study the immune response in asthma are limited by bulk profiling of cells. Our objective was to Characterize a model of peripheral blood mononuclear cells (PBMCs) of patients with severe asthma (SA) and its response to the TLR3 agonist Poly I:C using two single-cell methods. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

16 Samples

Download data: CSV

(Submitter supplied) MicroRNAs (miRNAs) have emerged as important players in the regulation of T-cell functionality. However, comprehensive insight into the extent of age-related miRNA changes in T cells is lacking. We established miRNA expression patterns of CD45RO- naïve and CD45RO+ memory T-cell subsets isolated from peripheral blood cells from young and elderly individuals. Unsupervised clustering of the miRNA expression data revealed an age-related clustering in the CD45RO- T cells, while CD45RO+ T cells clustered based on expression of CD4 and CD8. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL8227

8 Samples

Download data: TXT

(Submitter supplied) screenning the differentially expressed genes of Jurkat-FF3, Jurkat-miR146a, Jurkat-miR146a-sponge cell lines.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

3 Samples

Download data: CEL, CHP

(Submitter supplied) We screened the differential expressed microRNA from the rheumatoid arthritis patients and healthy person in their hydrops articuli CD4+ T cells.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL10268

3 Samples

Download data

(Submitter supplied) Systemic lupus erythematosus (SLE) is a chronic relapsing autoimmune disease characterized by the production of autoantibodies and multiple organ involvement. In this study, we investigated genome-wide DNA methylation changes in the CD8+ T cells from 8 pairs of lupus patients compared to age, sex, and ethnicity matched healthy controls.

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platform:

GPL23976

16 Samples

Download data: IDAT, TXT

(Submitter supplied) MicroRNAs are a major class of gene regulators in mammals. While numerous aspects of the immune systems are controlled by miRNAs, their precise role in the CD8+ T cell response remains unclear. In this report, we show that miR-150 is the most abundant miRNA expressed in CD8+ T cells and its expression is required for proper effector cell differentiation in response to acute and chronic pathogens. In the absence of miR-150, CD8+ T cells failed to both undergo robust expansion and differentiate into short-lived terminal effector cells. The lack of miR-150 also altered the effector CD8+ T cell transcriptome such that, despite activation, genes associated with naïve or memory cells were highly expressed. The deletion of miR-150 also reduced killing efficiency of CD8+ T cells. These results uncover a cell-intrinsic role for miR-150 in the regulation of CD8+ T cell effector fate and function.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL13112

8 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL15018 GPL13607

24 Samples

Download data: TXT

(Submitter supplied) miRNA expression was determined using the Agilent Human miRNA Microarray G4870A (miRNA ID version)

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL15018

12 Samples

Download data: TXT

(Submitter supplied) mRNA expression was determined using the Agilent-G4851A SurePrint G3 Human GE 8x60K Microarray

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13607

12 Samples

Download data

(Submitter supplied) To further development of MG-related lncRNA expression differences, we have employed whole lncRNA microarray expression profiling as a discovery platform to identify MG-related lncRNA with the potential to distinguish MG.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL26963

16 Samples

Download data: TXT

(Submitter supplied) Here were provide RNA-seq from C57BL6/J mouse antigen-specific CD4+ T cells (naïve and activated) that have or lack expression of miR150

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

10 Samples

Download data: CSV