GEO DataSets

Analysis of LCM-isolated prechondrocytes in chondrogenic condensations and total limb cells at week 6 of embryogenesis. Results provide insight into molecular mechanisms distinguishing prechondrocytes from other cell lineages in developing limbs.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 cell type sets

Platform:

GPL570

Series:

GSE51812

12 Samples

Download data: CEL

(Submitter supplied) Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult stem/progenitor cells have been unsuccessful. We hypothesized that recapitulation of the human developmental chondrogenic program using pluripotent stem cells (PSCs) may represent a superior approach for cartilage restoration. Using laser capture microdissection followed by microarray analysis, we first defined a surface phenotype (CD146low/negCD166low/negCD73+CD44lowBMPR1B+) distinguishing the earliest cartilage committed cells (pre-chondrocytes) at 5-6 weeks of development; pellet assays confirmed these cells as functional, chondrocyte-restricted progenitors. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS5045 GDS5046

Platform:

GPL570

15 Samples

Download data: CEL

Analysis of LCM-isolated prechondrocytes in chondrogenic condensations at week 6 of embryogenesis and articular chondrocytes at week 17 of development. Results provide insight into molecular markers during the process of prechondrocyte maturation.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 cell type, 2 development stage sets

Platform:

GPL570

Series:

GSE51812

9 Samples

Download data: CEL

(Submitter supplied) Here we present RNA sequencing data of 2 clonal cell lines (P3D8 and P3E3) treated with BMP2 and BMP9 to differentiate cartilage and control untreated clonal cells

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23479

12 Samples

Download data: XLSX

(Submitter supplied) We performed β-catenin ChIP-seq, RARα ChIP-seq, and ATAC-seq on the hiPSC-derived cells in the standard or altered 2C differentiation protocols.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL20795

20 Samples

Download data: BED

(Submitter supplied) We performed open chromatin analysis and genome-wide analysis for RAR-DNA association, on the hiPSC-derived cells in the 2C protocol. The purposes were to analyze sequential opening and closing of chromatin elements, and to elucidate the molecular roles of RAR, in each differentiation stage of the 2C protocol.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL20795

35 Samples

Download data: BED

(Submitter supplied) To identify gene expression profiles during chondrocyte differentiation from human iPS cells by the 2C method

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL21185

18 Samples

Download data: TXT

(Submitter supplied) In-vitro differentiation of chondrocyte populations recapitulate in-vivo behaviours, and reveal gene regulatory networks involved in chondrocyte development

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL20301 GPL18573 GPL19057

46 Samples

Download data: BED, NARROWPEAK, TXT, XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

48 Samples

Download data: MTX, TSV, TXT

(Submitter supplied) It has been notoriously challenging for researchers in the fields of stem cell biology and regenerative medicine to robustly generate uniform populations of differentiated cells from human induced pluripotent stem cells (hiPSCs). In particular, the process of iPSC chondrogenesis has been difficult to reproduce among different laboratories. While several differentiation protocols are being developed, few, if any, yield homogenous hiPSC-derived chondrocytes without any off-target contaminating cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

36 Samples

Download data: TXT

(Submitter supplied) It has been notoriously challenging for researchers in the fields of stem cell biology and regenerative medicine to robustly generate uniform populations of differentiated cells from human induced pluripotent stem cells (hiPSCs). In particular, the process of iPSC chondrogenesis has been difficult to reproduce among different laboratories. While several differentiation protocols are being developed, few, if any, yield homogenous hiPSC-derived chondrocytes without any off-target contaminating cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

12 Samples

Download data: MTX, TSV

(Submitter supplied) Objectives Traditional approaches to study Progressive Pseudorheumatoid Arthropathy of Childhood (PPAC) failed to uncover how loss-of-function mutations in Wnt inducible secreted protein 3 (WISP3) cause premature cartilage failure in children. We developed a human induced pluripotent stem cell (hiPSC)-based model of cartilage development to elucidate pathological changes in WISP3-deficient articular cartilage tissues compared to WISP3-sufficient isogenic cartilage tissues. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20301 GPL24676

6 Samples

Download data: CSV, TAR

(Submitter supplied) Bulk RNA-seq is used to study transcriptomic differences between WISP3-deficient and WISP3-sufficient articular cartilage derived from hPSCs in vitro

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20301 GPL18573

18 Samples

Download data: CSV, XLSX

(Submitter supplied) Comparison of gene expression profiles during differentiation from pluripotent state to limb bud state.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

18 Samples

Download data: XLSX

(Submitter supplied) The synovial joint forms from a pool of progenitor cells in the interzone region of the future joint. Expression of Gdf5 and Wnt9a has been used to mark the earliest cellular processes in the formation of the interzone and the progenitor cells. However, progression and lineage specification toward the different tissues of the joint is not well understood. Here, by lineage tracing studies we identify a population of Lgr5+ interzone cells that contribute to the formation of cruciate ligaments, synovial membrane and articular chondrocytes of the joint. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18480

1 Sample

Download data: MTX, TSV

(Submitter supplied) Nascent embryonic joints, interzones, contain a distinct cohort of progenitor cells responsible for the formation of the majority of articular tissues. However, to date the interzone has largely been studied using in situ analysis for candidate genes in the context of the embryo rather than using an unbiased genome wide expression analysis on isolated interzone cells, leaving significant controversy regarding the exact role of the intermediate and outer interzone layers in joint formation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13912

6 Samples

Download data: TXT