GEO DataSets

Analysis of hepatic stellate cells (HSCs) cultured with LPS for 1, 3, 6, 12, 24 hr. During exposure to toxins such as LPS, the liver suffers inflammatory injury. Results provide insight into molecular mechanisms underlying hepatic inflammatory and immune responses.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent, 5 time sets

Platform:

GPL1355

Series:

GSE49980

11 Samples

Download data: CEL

(Submitter supplied) Located in the perisinusoidal space of Disse, hepatic stellate cells (HSCs) communicate with all other liver cell types by physical association and / or by producing cytokines and chemokines. In liver disease and folllowing liver transplantation, elevated levels of endotoxin (bacterial lipopolysaccharide: LPS) stimulate HSCs to produce increased amounts of cytokines and chemokines. Transcriptomic analysis of cultured HSCs stimulated with LPS yields a survey of expression changes which potentially modulate the hepatic inflammatory and immune responses.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Datasets:

GDS5251 GDS5252

Platform:

GPL1355

11 Samples

Download data: CEL, TXT

Analysis of hepatic stellate cells (HSCs) cultured with LPS for 1 and 24 hr. During exposure to toxins such as LPS, the liver suffers inflammatory injury. Results provide insight into molecular mechanisms underlying hepatic inflammatory and immune responses.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent, 2 time sets

Platform:

GPL1355

Series:

GSE49980

8 Samples

Download data: CEL

(Submitter supplied) Aged humans and rodents are susceptible to infection with Streptococcus pneumoniae bacteria as a result of an inability to make antibodies to capsular polysaccharides. This is partly a result of decreased production of proinflammatory cytokines and increased production of interleukin (IL)-10 by macrophages (Mphi) from aged mice. To understand the molecular basis of cytokine dysregulation in aged mouse Mphi, a microarray analysis was performed on RNA from resting and lipopolysaccharide (LPS)-stimulated Mphi from aged and control mice using the Affymetrix Mouse Genome 430 2.0 gene chip. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL

(Submitter supplied) Chronic, low-grade inflammation has a widespread and significant impact on health, especially in Western-society. While inflammation is beneficial for the removal of microbes, low-grade inflammation never resolves and can cause or worsen other diseases. However, the process by which low-grade inflammation occurs is poorly understood. As lipopolysaccharide (LPS) is associated with chronic inflammatory diseases, we exposed murine bone-marrow derived monocytes to chronic LPS-stimulation at Low-dose (100pg/mL) or High-dose (1 µg/mL), as well as a PBS control. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL21103

45 Samples

Download data: BW, TXT