GEO DataSets

Analysis of DBC1-knockout splenic B cells following anti-CD40 activation. The deleted in breast cancer 1 (DBC1) gene was initially identified as part of the 8p22 chromosome region that is deleted in breast cancer. Results provide insight into the role of DBC1 in B cell function.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 4 protocol sets

Platform:

GPL6885

Series:

GSE61029

8 Samples

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(Submitter supplied) DBC1 KO B cells were either unstimulated, activated with anti-IgM, anti-CD40 of LPS for 4 hours. Genes differentially expressed in DBC1 KO B cells were compared to WT B cells.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5428

Platform:

GPL6885

8 Samples

Download data: TXT

(Submitter supplied) Malignant cells of Hodgkin's lymphoma (HL) cells are characterized by constitutive activation of the canonical as well as the non-canonical NF-κB signaling cascades. Knockdown of a subunit combination corresponding to the non-canonical NF-κB dimer (p52/RelB) in the HL cell line L-1236 caused up-regulation of a set of genes that are associated with hematopoietic and lymphoid organ development. As p52 can form homodimeric complexes, which can repress transcription either alone or in association with transcriptional repressors such as HDAC1, we knocked down p52 alone to analyze its role in gene repression in HL cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

12 Samples

Download data: CEL

(Submitter supplied) Malignant Hodgkin's lymphoma (HL) cells are characterized by constitutive activation of the canonical as well as the non-canonical NF-κB signaling cascades. We depleted subunit combinations corresponding to either canonical (p50/RelA) or non-canonical (p52/RelB) dimers in the HL cell line L-1236 and performed Affymetrix microarray analysis. Knockdown of p52/RelB affected the expression of a significantly higher number of genes than the knockdown of p50/RelA. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

24 Samples

Download data: CEL

(Submitter supplied) The malignant cells of Hodgkin's lymphoma are characterized by a constitutive activation of the canonical as well as the non-canonical NF-κB signaling cascades. We carried out genome-wide localization and expression profiling experiments in the Hodgkin lymphoma cell line L1236 for the canonical and non-canonical NF-κB pathway components p65, p50 and p52, RelB, respectively. We found that the single NF-κB subunits bind to overlapping, but distinct cistromes by using consensus motifs of high similarity.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL11154 GPL9115

14 Samples

Download data: TXT

(Submitter supplied) Upon encounter with antigen, mature B cells undergo complex changes in their physiological state and anatomical localization, and initiate a differentiation process that ultimately produces antibody-secreting cells (ASCs), a major effector population of the immune system. To better characterize this process we have defined the transcriptome of all stages of B cell terminal differentiation by performing a large scale RNA-seq experiment. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

31 Samples

Download data: TXT

(Submitter supplied) Purpose: The role of intrinsic apoptosis in mature B cell homeostasis is still elusive. The aim of this study is to compare the transcriptome of mature B cells that are either sensitive or resistant to intrinsic apoptosis. Methods: Bone marrow-residing Mature B cells were FACS-sorted as B220high CD93- IgM+ with >99.8% purity. RNA was extracted with Qiagen RNA extraction kit.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16417

12 Samples

Download data: TXT

(Submitter supplied) In order to understand which pathways are involved and what genes are affected when small T is expressed in mammalian cells, we carried out microarray analaysis in these cell lines Total cellulatr RNA was isolated at 20 hrs after doxycycline addition in samples 2,4 and 6 (+DOX), and was subjecyed to microarray analysis using Affymetrix Human Genome U133 Plus 2.0 (HG-U133_Plus_2) chips.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data: CEL