GEO DataSets

Comparison of retinoblastoma-deficient (Rb-/-) and control wild type prostate epithelial cell lines maintained in RPMI-1640 medium supplemented with 5% fetal bovine serum, or without serum for 48 hours.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell line, 2 growth protocol sets

Platform:

GPL81

Series:

GSE934

9 Samples

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(Submitter supplied) Comparison of control wild-type and Rb-/- prostate epithelial cell lines under untreated and serum-free conditions Keywords = prostate Keywords = epithelial Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS575

Platform:

GPL81

9 Samples

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(Submitter supplied) The nuclear LIM-only protein LMO4 is upregulated in breast cancer, especially estrogen receptor negative tumors, and its overexpression in mice leads to hyperplasia and tumor formation. Here, we show that deletion of LMO4 in the mammary glands of mice leads to impaired lobuloalveolar development due to decreased epithelial cell proliferation. With the goal of discovering potential LMO4-target genes, we also developed a conditional expression system in MCF-7 cells for both LMO4 and a dominant negative (DN) form of its co-regulator, Co-factor of LIM domains (Clim/Ldb/Nli). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS2787 GDS2788 GDS2789

Platforms:

GPL96 GPL97 GPL570

18 Samples

Download data: CEL

Analysis of breast cancer MCF-7 cells after the induction of expression of a dominant negative form of the cofactor of LIM domains (CLIM), a co-regulator of the nuclear LIM only protein 4 (LMO4). LMO4 is upregulated in breast cancer. Results provide insight into the role of LMO4 in tumor formation.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL570

Series:

GSE7382

6 Samples

Download data: CEL

Analysis of breast cancer MCF-7 cells following the induction of expression of nuclear LIM-only protein 4 (LMO4). LMO4 is upregulated in breast cancer. Results provide insight into the role of LMO4 in promoting tumor formation.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL97

Series:

GSE7382

6 Samples

Download data: CEL

Analysis of breast cancer MCF-7 cells following the induction of expression of nuclear LIM-only protein 4 (LMO4). LMO4 is upregulated in breast cancer. Results provide insight into the role of LMO4 in promoting tumor formation.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL96

Series:

GSE7382

6 Samples

Download data: CEL

(Submitter supplied) Identification of E2F1-regulated genes that modulate the transition from quiescence into DNA synthesis, or have roles in apoptosis, signal transduction, membrane biology, and transcription repression. Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS46

Platform:

GPL75

4 Samples

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Identification of E2F1-regulated genes that modulate the transition from quiescence into DNA synthesis, or have roles in apoptosis, signal transduction, membrane biology, and transcription repression.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL75

Series:

GSE498

4 Samples

Download data

(Submitter supplied) previously published mouse data Pubmed IDS: 12754511, 12839964 Keywords: disease state analysis

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL75 GPL76

56 Samples

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(Submitter supplied) The specific ablation of Rb1 gene in epidermis (RbF/F;K14cre) promotes proliferation and altered differentiation but does not produce spontaneous tumour development. These phenotypic changes are associated with increased expression of E2F members and E2F-dependent transcriptional activity. Here, we have focused on the possible dependence on E2F1 gene function. We have generated mice that lack Rb1 in epidermis in an inducible manner (RbF/F;K14creERTM). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

20 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL17021 GPL1261

56 Samples

Download data: BED, CEL

(Submitter supplied) To explore how Myc and E2f3 might coordinate gene expression programs that drive normal cell cycles in wild type intestines and ectopic cell cycles in Rb-deficient small intestines, we compared Myc and E2f3 chromatin occupancy in these tissues.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17021

8 Samples

Download data: BED

(Submitter supplied) Loss of Myc corrects abrrant transcription in Rb KO villi, while these genetic manipulation does not lead to major gene expression changes in crypts. We used Affymetrix microarrays to profile the global gene expression changes caused by loss of Rb and Rb/Myc.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

30 Samples

Download data: CEL

(Submitter supplied) Combined ablation of Myc and E2f1-3 results in disruption of crypt-villus integrity in the small intestine due to a S-G2 cell cycle blockade. We used Affymetrix microarrays to profile the global gene expression changes caused by loss of Myc and/or E2f1-3.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

18 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL11154

9 Samples

Download data: TAB

(Submitter supplied) Zinc finger and BTB domain containing transcription repressor ZBTB7A has been recently reported as a tumor suppressor who plays important functions to prevent the progression of prostate cancer. However, the chromatin activity of ZBTB7A in prostate cancer cells remain unclear. In order to identify the cistrome and transcriptome of ZBTB7A, we performed ZBTB7A ChIP-seq in VCaP cells and RNA-seq in VCaP cells transfected with siRNA targeting ZBTB7A or non-targeting control, respectively (cells were grown in full serum). more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

4 Samples

Download data: TAB

(Submitter supplied) Zinc finger and BTB domain containing transcription repressor ZBTB7A has been recently reported as a tumor suppressor who plays important functions to prevent the progression of prostate cancer. However, the chromatin activity of ZBTB7A in prostate cancer cells remain unclear. In order to identify the cistrome and transcriptome of ZBTB7A, we performed ZBTB7A ChIP-seq in VCaP cells and RNA-seq in VCaP cells transfected with siRNA targeting ZBTB7A or non-targeting control, respectively (cells were grown in full serum). more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL11154

5 Samples

Download data: BW, NARROWPEAK

(Submitter supplied) The E2F family consists of transcriptional repressors and activators that control cell proliferation. In the classic paradigm of cell cycle regulation, the three activators, E2F1, E2F2 and E2F3, are invariably depicted as the final components of a CDK/Rb signaling cascade that executes the transcriptional program necessary to commit cells to enter S phase. Unexpectedly, we find through analysis of Affymetrix expression array data that mature lens epithelial cells deficient for E2F1-3 fail to repress cell cycle-regulated genes (and other targets of E2F) and that this corresponds with subsequent apoptosis and cellular collapse in the lens.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

20 Samples

Download data: CEL

(Submitter supplied) The retinoblastoma protein (RB) is preferentially lost in the progression to castrate resistant prostate cancer (CRPC). However, the alterations associated with such loss have been scantly described. Current findings have identified a novel E2F1 associated cistrome and transcriptome that is associated with RB loss in PCa. In order to determine the contribution of chromatin accessibility to alterations in E2F1 activity, ATAC-Seq was performed.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL18573

6 Samples

Download data: BED

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL18573

14 Samples

Download data: BED