GEO DataSets

Analysis of differentiated C3H10T1/2 fibroblasts treated with 10 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and/or 10 nM EGF. Prior to treatment, cells induced to differentiate into adipocytes with IDMB hormone. EGF increases effectiveness of TCDD in suppressing hormone-induced adipogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 8 agent sets

Platform:

GPL81

Series:

GSE1123

18 Samples

Download data: CEL

(Submitter supplied) Confluent C3H10T1/2 cells stimulated to differentiate using IDMB (1uM BRL49653, 1uM Dexamethasone, 0.5uM IBMX, 10ug/mL Insulin). Expression was examined 24 hours after IDMB treatment. Additionally, the effects of addition of TCDD 48 hours prior to IDMB treatment, and EGF addition concurrent with IDMB treatment were examined. Keywords = Adipogenesis Keywords = TCDD Keywords = EGF Keywords: repeat sample

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS951

Platform:

GPL81

18 Samples

Download data: CEL

(Submitter supplied) Conventional biochemical and molecular techniques identified previously several genes whose expression is regulated by the aryl hydrocarbon receptor (AHR). We sought to map the complete spectrum of AHR-dependent genes in male adult liver using expression arrays to contrast mRNA profiles in Ahr-null mice (Ahr–/–) with those in mice with wild-type AHR (Ahr+/+). Transcript profiles were determined both in untreated mice and in mice treated 19 h earlier with 1000 µg/kg 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

17 Samples

Download data: CEL, DAT, EXP

(Submitter supplied) Profiling of mRNA abundances with high-throughput platforms such as microarrays and RNA-Seq has become an important tool in both basic and biomedical research. However these platforms remain prone to systematic errors, and have challenges in clinical and industrial application. As a result it is standard practice to validate a subset of key results using alternate technologies. Similarly, clinical and industrial applications typically involve transitions from high-throughput discovery platform to medium-throughput validation ones. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array; Expression profiling by RT-PCR

Platforms:

GPL16146 GPL16444 GPL16145

458 Samples

Download data: TXT

(Submitter supplied) Pregnant C57Bl6N mice were treated with 0 (corn oil), 1.5, 3.0, or 6.0 ug/kg TCDD on gd14.5. Fetal hearts were collected on gd17.5. Hearts from each litter were pooled onto one chip. 4 replicates of each condition were run on affymetrix MG_U74Av2 chips, using standard affymetrix protocols and controls. Keywords: dose-response, 3 doses plus corn oil control, 4 replicates

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1302

Platform:

GPL81

20 Samples

Download data: CEL

Analysis of gestational day (GD) 17.5 fetal hearts isolated from pregnant C57BL/6Ns treated at GD 14.5 with up to 6.0 ug/kg 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). TCDD, a cardiovascular teratogen, inhibits cardiomyocyte proliferation, a process that peaks between GD 14.5 to 17.5.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 4 dose sets

Platform:

GPL81

Series:

GSE2812

20 Samples

Download data: CEL

(Submitter supplied) Over-activation of the aryl hydrocarbon receptor by TCDD in mice leads among other phenotypes to a severe thymic atrophy accompanied by immunosuppression. TCDD causes a block in thymocyte maturation and a preferential emigration of immature CD4-CD8- DN thymocytes (recent thymic emigrants) into the periphery. As part of this study gene expression profiles from DN thymocytes and thymic emigrants were generated from TCDD and solvent control mice Keywords: Affymetrix, TCDD, CD4-CD8- thymocytes, Thymus involution, thymic emigration, Ahr

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL339

8 Samples

Download data: CEL

(Submitter supplied) In many mammals, halogenated aromatic hydrocarbon (HAH) exposure causes wasting syndrome, defined as lethal weight loss as a result of severe and persistent hypophagia. The most potent HAH in causing wasting is 2,3,7,8-tetrachlorodibenzo-ρ-dioxin (TCDD), which exerts its toxic effects through the aryl hydrocarbon receptor (AHR) – a transcription factor. Because TCDD toxicity is thought to predominantly arise from dysregulation of AHR-transcribed genes, we hypothesized that wasting syndrome is due to TCDD-induced dysregulation of genes involved in regulation of food-intake. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL19144

16 Samples

Download data: CEL, TXT

(Submitter supplied) We profiled hepatic transcriptional responses of 6 strains of rats with varying sensitivity to a dioxin, TCDD, at 19 hours following exposure. The resistant rats exhibited significantly reduced transcriptional responses in comparison to the sensitive strains. We hypothesize that genes which show differential changes between the resistant and sensitive rats may potentially explain sensitivity.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platforms:

GPL14123 GPL14122

56 Samples

Download data: CEL

(Submitter supplied) Over activation of the aryl hydrocarbon receptor (AhR) by TCDD results ampng other phenotypes in severe thymic atrophy accompanied by immunosuppression. The link between thymic atrophy, skewed thymocyte differntiation and immunosuppression is still not fully resolved. This study investigates the TCDD elicted exprssion changes in the ET, cortical thymus epithelial cell line. Keywords: TCDD, AhR, thymic epithelial cells, thymic involution, thymus atrophy

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL8321

12 Samples

Download data: CEL

(Submitter supplied) TCDD increased expression of numerous differentiation specific genes and decreased expression of numerous genes involved in mitochondrial health and redox homeostasis

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

8 Samples

Download data: CEL

(Submitter supplied) 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) has a large number of biological effects, including skin, cardiovascular, neurologic disease, diabetes, infertility and cancer. We analysed the in vitro TCDD effects on human CD34+ cells and tested the gene expression modulation by means of microarray analyses before and after TCDD exposure. We identified 253 differentially modulated probe sets, identifying 217 well-characterized genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3889

Platform:

GPL96

8 Samples

Download data: CEL

Analysis of hematopoietic progenitor CD34+ cells from 5 healthy donors (3 paired samples) before and after in vitro 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure. Results provide insight into the molecular pathways of TCDD-induced hematopoietic progenitor cell impairment in bone marrow.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 5 individual sets

Platform:

GPL96

Series:

GSE24193

8 Samples

Download data: CEL

(Submitter supplied) Genome-wide DNA methylation profiling was performed in human mesenchymal stem cells (hMSCs) differentiated into adipocytes (day 10) while being continuously exposed to either one of three different persistent organic pollutants (POPs), namely TCDD, PFOS, and TBT. The Illumina Infinium 450K Human DNA methylation Beadchip v1.2 was used to measure DNA methylation of unexposed differentiated adipocytes and compared to POP-exposed differentiated adipocytes. more...

Organism:

Homo sapiens

Type:

Methylation profiling by genome tiling array

Platform:

GPL13534

15 Samples

Download data: IDAT

(Submitter supplied) Adult zebrafish can completely regenerate their caudal fin following amputation. This complex process is initiated by the formation of an epithelial would cap over the amputation site by 12 hours post amputation (hpa). Once the cap is formed, mesenchymal cells proliferate and migrate from sites distal to the wound plane and accumulate under the epithelial cap forming the blastemal structure within 48 hpa. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Dataset:

GDS1797

Platform:

GPL1319

18 Samples

Download data: CEL

Analysis of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposed caudal fins at various time points up to 5 days post amputation. TCDD prevents caudal fin regeneration. Results provide insight into the pathways required for fin regeneration and the mechanisms of TCDD toxicity.

Organism:

Danio rerio

Type:

Expression profiling by array, count, 2 agent, 3 time sets

Platform:

GPL1319

Series:

GSE3667

18 Samples

Download data: CEL