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Niemann-Pick type C fibroblast analysis

(Submitter supplied) Primary skin fibroblasts from four Niemann-Pick type C patients homozygous for the I1061T mutation and four control individuals were cultured under identical conditions in DMEM containing 10% fetal bovine serum. Cells were harvested at 50-70% confluency. mRNA was isolated with the FastTrack 2.0 mRNA isolation kit according to the manufacturer's instructions (Invitrogen, Carlsbad, CA). A reference RNA comprised of 10 cell lines was used as the control for each hybridized sample (Stratagene, La Jolla, CA). more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL5742
8 Samples
Download data
Series
Accession:
GSE8770
ID:
200008770

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