U.S. flag

An official website of the United States government

Format
Sort by

Send to:

Choose Destination

Links from SRA

Items: 2

1.

dCas9+3A RRBS

Organism:
Homo sapiens
Source name:
HEK 293T
Platform:
GPL11154
Series:
GSE97813 GSE97816
Download data: TXT
Sample
Accession:
GSM2664250
ID:
302664250
2.

DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A [RRBS]

(Submitter supplied) We demonstrate that dCas9-SunTag-DNMT3A dramatically increased CpG methylation at the HOXA5 locus in human embryonic kidney 293T cells (HEK293T). Furthermore, using a single sgRNA, dCas9-SunTag-DNMT3A was able to methylate a 4.5 kb genomic region and repress HOXA5 gene expression. Reduced representation bisulfite sequencing (RRBS) and RNA-seq showed that dCas9-SunTag-DNMT3A methylated regions of interest with minimal impact on the global DNA methylome and transcriptome.
Organism:
Homo sapiens
Type:
Methylation profiling by high throughput sequencing
Platform:
GPL11154
4 Samples
Download data: TXT
Series
Accession:
GSE97813
ID:
200097813

Supplemental Content

db=gds|term=|query=1|qty=2|blobid=MCID_675ef3adedcc291f246174f4|ismultiple=true|min_list=5|max_list=20|def_tree=20|def_list=|def_view=|url=/Taxonomy/backend/subset.cgi?|trace_url=/stat?
   Taxonomic Groups  [List]
Tree placeholder
    Top Organisms  [Tree]

Find related data

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Support Center