GEO DataSet Links for SRA (Select 4160654) - GEO DataSets

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Items: 2

Select item 3026642521.

dCas9+3A+HOXA5 RRBS

Organism:: Homo sapiens

Source name:: HEK 293T

Platform:: GPL11154
Series:: GSE97813 GSE97816

Download data: TXT

Sample

Accession:: GSM2664252

ID:: 302664252

SRA Run Selector

Select item 2000978132.

DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A [RRBS]

(Submitter supplied) We demonstrate that dCas9-SunTag-DNMT3A dramatically increased CpG methylation at the HOXA5 locus in human embryonic kidney 293T cells (HEK293T). Furthermore, using a single sgRNA, dCas9-SunTag-DNMT3A was able to methylate a 4.5 kb genomic region and repress HOXA5 gene expression. Reduced representation bisulfite sequencing (RRBS) and RNA-seq showed that dCas9-SunTag-DNMT3A methylated regions of interest with minimal impact on the global DNA methylome and transcriptome.