GEO DataSets

(Submitter supplied) Microbial deconstruction of plant polysaccharides is important for environmental nutrient cycling, and bacteria proficient at this process have extensive suites of polysaccharide-specific enzymes. In the Gram-negative saprophyte Cellvibrio japonicus, genome annotation suggests that 17 genes are predicted to encode Carbohydrate-Active enZymes (CAZymes) with roles in cellulose degradation, however previous work suggested that only a subset of these genes is essential. more...

Organism:

Cellvibrio japonicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22758

6 Samples

Download data: XLSX

(Submitter supplied) Bacteria are major drivers of organic matter decomposition and play crucial roles in global nutrient cycling. Although the degradation of dead fungal biomass (necromass) is increasingly recognized as an important contributor to soil carbon (C) and nitrogen (N) cycling, the genes and metabolic pathways involved in necromass degradation are under characterized. In particular, how bacteria degrade necromass containing different quantities of melanin, which largely control rates of necromass decomposition in situ, is largely unknown. more...

Organism:

Chitinophaga pinensis; Cellvibrio japonicus; Serratia marcescens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL34520 GPL34518 GPL34519

48 Samples

Download data: CSV, XLSX

(Submitter supplied) Recent interest in rare α-diglucosides such as kojibiose (α-1,2), nigerose (α-1,3), and isomaltose (α-1,6) reflects developments in pharmaceutical, biotechnological, and culinary industries that value these sugars as prebiotics, carrier molecules, and low glycemic index sweeteners. There have been only a few enzymes capable of degrading these substrates characterized, largely due in part to difficulties in identifying potential targets based solely on bioinformatic predictions. more...

Organism:

Cellvibrio japonicus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL28467 GPL22758

12 Samples

Download data: XLSX

(Submitter supplied) Plant mannans are a component of lignocellulose that may possess diverse compositions changing both in terms of its backbone and side-chain substitutions. Consequently, the degradation of mannan substrates requires a cadre of enzymes for complete reduction to substituent monosaccharides, specifically mannose, galactose, and/or glucose. One bacterium that possesses this suite of enzymes is the Gram-negative saprophyte Cellvibrio japonicus, which has ten predicted mannanases from the Glycoside Hydrolase (GH) families 5, 26, and 27. more...

Organism:

Cellvibrio japonicus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL28467 GPL22758

12 Samples

Download data: XLSX

(Submitter supplied) Chitin utilization by microbes plays a significant role in cycling of carbon and nitrogen in the biosphere, and the study of the microbial approaches used to degrade chitin will facilitate our understanding of bacterial strategies to degrade this recalcitrant polysaccharide. The early stages of chitin depolymerization by the bacterium Cellvibrio japonicus have been characterized and are dependent on one chitin-specific lytic polysaccharide monooxygenase and non-redundant glycoside hydrolases from the family GH18 to generate chito-oligosaccharides for entry into metabolism. more...

Organism:

Cellvibrio japonicus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL22758 GPL28467

24 Samples

Download data: XLS

(Submitter supplied) Background: Xyloglucan (XyG) is a ubiquitous and fundamental polysaccharide of plant cell walls. Due to its structural complexity, XyG requires a combination of backbone-cleaving and sidechain-debranching enzymes for complete deconstruction into its component monosaccharides. The soil saprophyte Cellvibrio japonicus has emerged as a genetically tractable model system to study biomass saccharification, in part due to an innate capacity to utilize a wide range of plant polysaccharides for growth. more...

Organism:

Cellvibrio japonicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22758

6 Samples

Download data: TXT

(Submitter supplied) Understanding the strategies used by bacteria to degrade polysaccharides constitutes an invaluable tool for biotechnological applications Bacteria are major mediators of polysaccharide degradation in nature, however the complex mechanisms used to detect, degrade, and consume these substrates are not well understood, especially for recalcitrant polysaccharides such as chitin It has been previously shown that the model bacterial saprophyte Cellvibrio japonicus is able to catabolize chitin, but little is known about the enzymatic machinery underlying this capability Previous analyses of the C japonicus genome and proteome indicated the presence of four family 18 Glycoside Hydrolase (GH18) enzymes, and studies of the proteome indicated that all are involved in chitin utilization Using a combination of in vitro and in vivo approaches, we have studied the roles of these four chitinases in chitin bioconversion Genetic analyses showed that only the chi18D gene product is essential for the degradation of chitin substrates Biochemical characterization of the four enzymes showed functional differences and synergistic effects during chitin degradation, indicating non-redundant roles in the cell Transcriptomic studies revealed complex regulation of the chitin degradation machinery of C japonicus and confirmed the importance of CjChi18D and CjLPMO10A, a previously characterized chitin-active enzyme With this systems biology approach, we deciphered the physiological relevance of the GH18 enzymes for chitin degradation in C japonicus, and the combination of in vitro and in vivo approaches provided a comprehensive understanding of the initial stages of chitin degradation by this bacterium

Organism:

Cellvibrio japonicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22758

6 Samples

Download data: TXT

(Submitter supplied) Lignocellulose degradation by microbes plays a central role in global carbon cycling, human gut metabolism, and renewable energy technologies While considerable effort has been put into understanding the biochemical aspects of lignocellulose degradation, much less work has been done to understand how these enzymes work in an in vivo context Here, we report a systems level study of xylan degradation in the saprophytic bacterium Cellvibrio japonicus Transcriptome analysis indicated seven genes that encode carbohydrate active enzymes were up-regulated during growth with xylan containing media In-frame deletion analysis of these genes found that only gly43F is critical for utilization of xylo-oligosaccharides, xylan, and arabinoxylan Heterologous expression of gly43F was sufficient for the utilization of xylo-oligosaccharides in Escherichia coli Additional analysis found that the xyn11A, xyn11B, abf43L, abf43K, and abf51A gene products were critical for utilization of arabinoxylan Furthermore, a predicted transporter (CJA_1315) was required for effective utilization of xylan substrates, and we propose this unannotated gene be called xntA (xylan transporter A) Our major findings are 1) C japonicus employs both secreted and surface associated enzymes for xylan degradation, which differs from the strategy used for cellulose degradation, and 2) a single cytoplasmic β-xylosidase is essential for the utilization of xylo-oligosaccharides

Organism:

Cellvibrio japonicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22758

6 Samples

Download data: TXT

(Submitter supplied) Degradation of polysaccharides forms an essential arc in the carbon cycle, provides a percentage of our daily caloric intake, and is a major driver in the renewable chemical industry. Microorganisms proficient at degrading insoluble polysaccharides possess large numbers of carbohydrate active enzymes, many of which have been categorized as functionally redundant. Here we present data that suggests that carbohydrate active enzymes that have overlapping enzymatic activities can have unique, non-overlapping biological functions in the cell. more...

Organism:

Cellvibrio japonicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22758

12 Samples

Download data: TXT

(Submitter supplied) Investigation of whole genome gene expression level changes in Cellvibrio japonicus wild-type, comparing glucose vs cellulose. Study was purposed with determining changes in polysaccharide degradation pathways during utilization of insoluble cellulose.

Organism:

Cellvibrio japonicus; Cellvibrio japonicus Ueda107

Type:

Expression profiling by array

Platform:

GPL19267

8 Samples

Download data: PAIR

(Submitter supplied) NimbleGen design name: Cellvibrio_japonicus_custom_NimbleGen_Array. An expression design for 3710 genes from Cellvibrio japonicus with 24-mer probe pairs (PM/MM) per gene. Each probe is replicated 3 times. The design includes random GC and other control probes. The Platform data table reflects a condensed representation of the expression array's replicate (SEQ_ID) features. The .ndf and .ngd files are linked below as supplementary files: Cellvibrio_japonicus_Array.ndf Cellvibrio_japonicus_Array.ngd Protocol: High-density DNA array with custom probes for Cellvibrio japonicus

Organism:

Cellvibrio japonicus

1 Series

8 Samples

Download data: NDF, NGD