GEO DataSets

(Submitter supplied) Understanding how novel complex traits originate is a foundational challenge in evolutionary biology. Yet how descent with modification in developmental evolution may lead to morphological innovation remains poorly understood. We investigated the origin of thoracic horns in scarabaeine beetles, one of the most dramatic classes of secondary sexual traits in the animal kingdom. We show that thoracic horns derive from bilateral source tissues, that diverse wing genes are functionally required for instructing this process, and that in the absence of Hox-input thoracic horn primordia transform to contribute to ectopic wings. more...

Organism:

Onthophagus taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22541

60 Samples

Download data: TSV

(Submitter supplied) The general morphology of the insect head has remained relatively unchanged through more than 400 million years of evolution. Yet throughout this period this same region has also become a hotspot for evolutionary novelty, yielding structures such as the eyestalks of stalk-eyed flies or the cephalic horns of dung beetles. How novelty can be integrated within ancient complex traits without disrupting the function and formation of that trait is a foundational, yet largely unresolved question in developmental and evolutionary biology. more...

Organism:

Onthophagus taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22541

66 Samples

Download data: TSV

(Submitter supplied) Purpose: Sexual dimorphisms fuel significant intraspecific variation and evolutionary diversification. Yet the developmental-genetic mechanisms underlying sex-specific development remain poorly understood. We focused on the conserved sex-determination gene doublesex (dsx) and the mechanisms by which it mediates sex-specific development in a species of horned beetle by combining systemic dsx knockdown, high-throughput sequencing of diverse tissues, and a genome-wide analysis of Dsx binding sites. more...

Organism:

Onthophagus taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22541

96 Samples

Download data: TSV

(Submitter supplied) This is a study on genome-wide transcriptional responses to nutritional variation and their dependency on trait and sex in the beetle Onthophagus taurus.

Organism:

Onthophagus taurus

Type:

Expression profiling by array

Platform:

GPL18805

48 Samples

Download data: PAIR, TXT

(Submitter supplied) Developmental mechanisms play an important role in determining the costs, limits, and evolutionary consequences of phenotypic plasticity. One issue central to these claims is the metaphor of developmental “decoupling,” where alternate morphs result from evolutionarily independent developmental pathways. We test this assumption through a microarray study that explores differences in gene expression between alternate morphs relative to differences between sexes, a classic example of developmental decoupling. more...

Organism:

Onthophagus nigriventris; Onthophagus taurus

Type:

Expression profiling by array

Platform:

GPL7555

71 Samples

Download data: GPR

(Submitter supplied) This series examines gene expression patterns in the head horns, thoracic horns, and legs of the horned beetles Onthophagus taurus. Expression in each of these tissues was compared to that in common non-appendage reference - abdominal epithelium.

Organism:

Onthophagus taurus

Type:

Expression profiling by array

Platform:

GPL7555

15 Samples

Download data: GPR

(Submitter supplied) This is a horned beetle (Onthophagus taurus) whole-genome expression array in HD2 12x135K format manufactured by Roche NimbleGen. 090921_OT_JC_EXP.noColon.ndf 090921_OT_JC_EXP.noColon.txt Protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. See manufacturer's website at http://www.nimblegen.com/.

Organism:

Onthophagus taurus

1 Series

48 Samples

Download data: NDF, TXT

(Submitter supplied) We mainly followed the protocol of Indiana University Drosophila Genomics Resource Center (Andrews et al. 2006, http://cgb.indiana.edu/publications/alias/dgrc1_prod_protocol/version/current) to print microarrays with a minor revision of post-print wash. Cleaned clones were dried completely, re-dissolved with DGRC spotting solution (1.5M Betaine in 3xSSC), and spotted on GAPSII MIcroarray Slides (Corning). more...

Organism:

Onthophagus taurus

2 Series

86 Samples

Download data