GEO DataSets

(Submitter supplied) To solve the problem of low FK520 production by Streptomyces hygroscopicus var. ascomyceticus FS35, PHB synthesis gene phaC and PHB decomposition gene fkbU were co-overexpressed in parent strain FS35 to construct recombinant strain OphaCfkbU. Surprisingly, recombinant strain OphaCfkbU accumulated more biomass than parent strain FS35 in whole fermentation. Therefore, to explore the effect of co-overexpression on the strain growth, comparative transcriptome analysis were carried out between parent strain FS35 and recombinant strain OphaCfkbU. more...

Organism:

Streptomyces hygroscopicus subsp. ascomyceticus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28015

2 Samples

Download data: FA, TXT

(Submitter supplied) For systematically improving geldanamycin yield, we investigated the gene expression profile on the fourth day during geldanamycin fermentation (geldanamycin began to largely accumulated on this day). According to the RNA-seq data, we deduced that the PKS genes gdmAI-AIII are rate-limiting genes for geldanamycin biosynthesis. We also found a strong endogeneous promoter according to the RNA-seq data and the reporter gene. more...

Organism:

Streptomyces hygroscopicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22787

1 Sample

Download data: TXT

(Submitter supplied) The RNA sequencing approach was utilized to carry out a comparative transcriptome analysis between Streptomyces hygroscopicus 5008 wild-type and a genetically engineered strain. The A-factor-like cascade play an important role in the regulation of validamycin biosynthesis by Streptomyces hygroscopicus 5008, and the pleiotropic regulator AdpA-H may positively regulate the transcription of gene cluster for the biosynthesis. more...

Organism:

Streptomyces hygroscopicus subsp. jinggangensis 5008

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18744

2 Samples

Download data: TXT

(Submitter supplied) To explore the molecular basis of validamycin overproduction at the transcriptional level, the transcriptomes of strain 5008 and TL01 cultivated in Yeast extract-Malt extract-Glucose (YMG) and rice-peanut cake based industrial (IND) fermentation medium were compared by microarray analysis.

Organism:

Streptomyces hygroscopicus subsp. jinggangensis TL01; Streptomyces hygroscopicus subsp. jinggangensis; Streptomyces hygroscopicus subsp. jinggangensis 5008

Type:

Expression profiling by array

Platform:

GPL15794

16 Samples

Download data: TXT

(Submitter supplied) To explore the molecular mechanism of the positive thermoregulation on Jinggangmycin A biosynthesis, the transcriptomes of S. hygroscopicus 5008 cultivated at 30℃ or 37℃ in liquid medium were compared by microarray analysis.

Organism:

Streptomyces hygroscopicus subsp. jinggangensis; Streptomyces hygroscopicus subsp. jinggangensis 5008

Type:

Expression profiling by array

Platform:

GPL15170

8 Samples

Download data: TXT

(Submitter supplied) Arrays of this design have barcodes that begin with 16025874 or 2525874. Protocol: see manufacturer's web site at http://www.agilent.com/

Organism:

Streptomyces hygroscopicus subsp. jinggangensis

1 Series

1 Related Platform

16 Samples

Download data

(Submitter supplied) Arrays of this design have barcodes that begin with 16025874 or 2525874. Protocol: see manufacturer's web site at http://www.agilent.com/

Organism:

Streptomyces hygroscopicus subsp. jinggangensis

1 Series

1 Related Platform

8 Samples

Download data: TXT