GEO DataSets

(Submitter supplied) Transcriptional profiling of Staphylococcus aureus comparing MW2 strain with SAN2 strain which was nisin A high resistant strain.

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus MW2

Type:

Expression profiling by high throughput sequencing; Expression profiling by array

Platform:

GPL21747

3 Samples

Download data: TXT

(Submitter supplied) Bacterial sepsis is a major killer in hospitalized patients. Coagulase-negative staphylococci (CNS) with the leading species Staphylococcus epidermidis are the most frequent causes of nosocomial sepsis, with most infectious isolates being methicillin resistant. However, which bacterial factors underlie the pathogenesis of CNS sepsis is unknown. While it has been commonly believed that invariant structures on the surface of CNS trigger sepsis by causing an over-reaction of the immune system, we show here that sepsis caused my methicillin-resistant S. more...

Organism:

Staphylococcus epidermidis; Staphylococcus aureus subsp. aureus MW2; Granulibacter bethesdensis; Staphylococcus epidermidis ATCC 12228; Chlamydia trachomatis D/UW-3/CX; Coxiella burnetii; Rickettsia rickettsii; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Chlamydia pneumoniae AR39; Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Chlamydia caviae GPIC; Staphylococcus haemolyticus JCSC1435

Type:

Expression profiling by array

Platform:

GPL4692

15 Samples

Download data: CEL, CHP

(Submitter supplied) To clarify the mechanism for the antibacterial activity of GR-SU, we performed the DNA microarray analysis.

Organism:

Staphylococcus aureus subsp. aureus MW2

Type:

Expression profiling by array

Platform:

GPL21747

4 Samples

Download data: TXT

(Submitter supplied) Virulence of many bacterial pathogens, including the important human pathogen Staphylococcus aureus, depends on the secretion of frequently high amounts of toxins. Toxin production involves the need for the bacteria to make physiological adjustments for energy conservation. While toxins are primarily known to be targets of gene regulation, such changes may be accomplished by regulatory functions of the toxins themselves. more...

Organism:

Chlamydia pneumoniae AR39; Coxiella burnetii RSA 493; Granulibacter bethesdensis; Borreliella burgdorferi B31; Chlamydia trachomatis D/UW-3/CX; Staphylococcus haemolyticus JCSC1435; Coxiella burnetii; Rickettsia rickettsii; Staphylococcus aureus; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Chlamydia caviae GPIC; Staphylococcus epidermidis ATCC 12228; Staphylococcus aureus subsp. aureus MW2

Type:

Expression profiling by array

Platform:

GPL4692

12 Samples

Download data: CEL, CHP

(Submitter supplied) The translation inhibitor Linezolid is an important antibiotic of last resort against multiresistant gram-positive pathogens including MRSA. Linezolid is reported to specifically inhibit extracellular virulence factors, but the molecular cause is unknown. To elucidate the physiological response of S. aureus to Linezolid in general and the possible inhibition of virulence factors specifically we performed a holistic study. more...

Organism:

Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus USA300

Type:

Expression profiling by array

Platform:

GPL7137

15 Samples

Download data: TXT

(Submitter supplied) A hallmark of Coxiella burnetii, the bacterial cause of human Q fever, is a biphasic developmental cycle that generates biologically, ultrastructurally, and compositionally distinct large cell variant (LCV) and small cell variant (SCV) forms. LCVs are replicating, exponential phase forms while SCVs are non-replicating, stationary phase forms. The SCV has several properties, such as a condensed nucleoid and an unusual cell envelope, suspected of conferring enhanced environmental stability. more...

Organism:

Rickettsia rickettsii; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Chlamydia caviae GPIC; Staphylococcus haemolyticus JCSC1435; Coxiella burnetii; Chlamydia trachomatis D/UW-3/CX; Chlamydia pneumoniae AR39; Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Staphylococcus epidermidis ATCC 12228; Staphylococcus aureus subsp. aureus MW2; Granulibacter bethesdensis

Type:

Expression profiling by array

Platform:

GPL4692

20 Samples

Download data: CEL, CHP

(Submitter supplied) Mutants from SG511 and NCTC8325 (wild type isolates) resistant to daptomycin were obtained following serial passage experiments in vitro. Transcriptomic experiments showed alteration in the expression of genes involved in various pathways including general metabolism, cell wall regulon and lipid metabolism.

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus USA300; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus COL

Type:

Expression profiling by array

Platform:

GPL7137

4 Samples

Download data: TXT

(Submitter supplied) Polymorphonuclear leukocytes (PMN) from patients with chronic granulomatous disease (CGD) fail to produce microbicidal concentrations of reactive oxygen species due to mutations in NOX2. Patients with CGD suffer from severe, life-threatening infections and inflammatory complications. Granulibacter bethesdensis is an emerging Gram-negative pathogen in CGD that resists killing by CGD PMN and inhibits PMN apoptosis through unknown mechanisms. more...

Organism:

Chlamydia pneumoniae AR39; Granulibacter bethesdensis; Homo sapiens; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Staphylococcus aureus subsp. aureus MW2; Coxiella burnetii; Rickettsia rickettsii; Staphylococcus epidermidis ATCC 12228; Chlamydia caviae GPIC; Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Chlamydia trachomatis D/UW-3/CX; Staphylococcus haemolyticus JCSC1435

Type:

Expression profiling by array

Platforms:

GPL4692 GPL570

123 Samples

Download data: CEL, CHP

(Submitter supplied) With a copy number of about 25,000 molecules per cell, Asp23 is one of the most abundant proteins in Staphylococcus aureus. Asp23 has been characterized as a protein with an apparent molecular mass of 23 kDa that, following an alkaline shock, accumulates in the soluble protein fraction. Moreover, it was shown that transcription of the asp23 gene is exclusively regulated by the alternative sigma factor B. more...

Organism:

Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus USA300

Type:

Expression profiling by array

Platform:

GPL7137

12 Samples

Download data: TXT

(Submitter supplied) A mutant of SG511 (wild type isolate), resistant to the presence of chitosan (polycationic molecule) was obtained following serial passage experiments resistance to chitosan exposition. Transcriptomic experiments showed alteration in the expression of genes involved in the cell wall regulon resulting in the modification of susceptibility phenotype against cell wall active antibiotics

Organism:

Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus USA300; Staphylococcus aureus subsp. aureus MW2

Type:

Expression profiling by array

Platform:

GPL7137

3 Samples

Download data: TXT

(Submitter supplied) Coxiella burnetii undergoes a biphasic developmental cycle within its host cell that generates morphologically and physiologically distinct large cell variants (LCV) and small cell variants (SCV). During the lag phase of the C. burnetii growth cycle, non-replicating SCV differentiate into replicating LCV that in turn differentiate back into SCV during stationary phase. Nearly homogeneous SCV are observed in infected Vero cells after extended incubation (21 to 28 days). more...

Organism:

Chlamydia pneumoniae AR39; Coxiella burnetii RSA 493; Granulibacter bethesdensis; Staphylococcus epidermidis ATCC 12228; Staphylococcus aureus subsp. aureus MW2; Coxiella burnetii; Rickettsia rickettsii; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Chlamydia caviae GPIC; Borreliella burgdorferi B31; Chlamydia trachomatis D/UW-3/CX; Staphylococcus haemolyticus JCSC1435

Type:

Expression profiling by array

Platform:

GPL4692

8 Samples

Download data: CEL, CHP

(Submitter supplied) Chlamydia trachomatis causes chronic inflammatory diseases of the eye and genital tract of global medical importance. The chlamydial plasmid plays an important role in the pathophysiology of these diseases as plasmid-deficient organisms are highly attenuated. The plasmid encodes both noncoding RNAs and eight conserved ORFs of undefined function. To understand plasmid gene function we generated plasmid shuttle vectors with deletions in each of the eight ORFs. more...

Organism:

Chlamydia muridarum; Staphylococcus epidermidis RP62A; Staphylococcus aureus subsp. aureus MW2; Staphylococcus haemolyticus JCSC1435; Coxiella burnetii; Rickettsia rickettsii; Chlamydia trachomatis; Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Chlamydia trachomatis D/UW-3/CX; Chlamydia pneumoniae AR39; Staphylococcus epidermidis ATCC 12228; Chlamydia caviae GPIC; Granulibacter bethesdensis

Type:

Expression profiling by array

Platform:

GPL4692

36 Samples

Download data: CEL, CHP

(Submitter supplied) Chlamydia trachomatis is a significant human pathogen yet their obligate intracellular nature severe restrictions upon research. Chlamydiae undergo a complex developmental cycle characterized by an infectious cell type known as the elementary body (EB) and an intracellular active replicative form called the reticulate body (RB). EBs have historically been described as metabolically dormant. A cell-free (axenic) culture system was developed which showed high levels of metabolic and biosynthetic activity from both EBs and RBs. more...

Organism:

Coxiella burnetii; Rickettsia rickettsii; Staphylococcus epidermidis ATCC 12228; Chlamydia pneumoniae AR39; Staphylococcus aureus subsp. aureus MW2; Granulibacter bethesdensis; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Chlamydia trachomatis; Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Chlamydia caviae GPIC; Chlamydia trachomatis D/UW-3/CX; Staphylococcus haemolyticus JCSC1435

Type:

Expression profiling by array

Platform:

GPL4692

20 Samples

Download data: CEL, CHP

(Submitter supplied) Methicillin resistant Staphylococcus aureus (MRSA) infection is becoming refractory to existing antibiotic therapy owing to the inherent ability of S. aureus to develop rapid resistance and is considered a major threat to public health. We found that a natural isolate of Bacillus pumilus from the Columbia River Estuary produces a strong anti-MRSA compound, amicoumacin A. As amicoumacin A has been reported to exhibit anti-microbial, anti-inflammatory, and anti-ulcer activities, we sought to uncover its mechanism of action. more...

Organism:

Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus USA300

Type:

Expression profiling by array

Platform:

GPL7137

9 Samples

Download data: TXT

(Submitter supplied) Objectives: Development of daptomycin resistance (DAPR) in Staphylococcus aureus is associated with clinical treatment failures. Mechanism(s) of such resistance has not been clearly defined. Methods: We studied an isogenic daptomycin-susceptible (DAPS) and daptomycin-resistant (DAPR) S. aureus strain pair (616; 701) from a patient with relapsing endocarditis during daptomycin treatment, using comparative transcriptomic and proteomic techniques. more...

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus USA300

Type:

Expression profiling by array

Platform:

GPL7137

6 Samples

Download data: TXT

(Submitter supplied) The alternative sigma factor σB of Staphylococcus aureus is involved in the coordination of the general stress response, expression of virulence determinants and modulation of antibiotic resistance levels. It controls a large regulon, either directly by recognizing conserved σB promoter sequences, or indirectly via σB-dependent elements. The σB-controlled yabJ-spoVG operon encodes two such putative downstream elements. more...

Organism:

Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus subsp. aureus USA300; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL7137

9 Samples

Download data: TXT

(Submitter supplied) Screening of various bisquaternary bisnaphthalimides against a variety of human pathogens revealed one compound, designated MT02, with strong inhibitory effects against gram-positive bacteria. The minimal inhibitory concentrations ranged from 0.31 µg/ml against community-acquired methicillin resistant Staphylococcus aureus (MRSA) strain USA300 to 20 µg/ml against Streptococcus pneumonia. DNA-microarray studies generated a transcriptional signature characterized by a strong increase of genes involved in DNA-metabolism, DNA-replication, SOS-response and transport of positively charged compounds. more...

Organism:

Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus USA300

Type:

Expression profiling by array

Platform:

GPL7137

2 Samples

Download data: PDF, TXT

(Submitter supplied) Methicillin-resistant Staphylococcus aureus (MRSA) is problematic both in hospitals and the community. Currently, we have limited understanding of mechanisms of innate immune evasion used by S. aureus. To that end, we created an isogenic deletion mutant in strain MW2 (USA400) of the saeR/S two-component gene regulatory system and studied its role in mouse models of pathogenesis and during human neutrophil interaction. more...

Organism:

Staphylococcus aureus; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Chlamydia caviae GPIC; Staphylococcus haemolyticus JCSC1435; Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Chlamydia trachomatis D/UW-3/CX; Chlamydia pneumoniae AR39; Staphylococcus epidermidis ATCC 12228; Staphylococcus aureus subsp. aureus MW2; Granulibacter bethesdensis; Coxiella burnetii; Rickettsia rickettsii

Type:

Expression profiling by array

Platform:

GPL4692

12 Samples

Download data: CEL, CHP

(Submitter supplied) Analysis of the intergenic regions of Staphylococcus aureus by bioinformatics predicted multiple regulatory regions. From this analysis, we also characterized 11 novel noncoding RNAs (RsaA-K) that are expressed in several S. aureus strains under different experimental conditions. Many of them accumulate in the late-exponential phase of growth. All ncRNAs are stable and their expression is Hfq-independent. more...

Organism:

Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus USA300; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus MSSA476

Type:

Non-coding RNA profiling by array

Platform:

GPL7137

2 Samples

Download data: TXT

(Submitter supplied) The genome sequence is the “blue-print of life”, but proteomics is required to relate this blue-print of life to the actual physiology of living cells. Because of their low complexity – only about 2.000 proteins make a Staphylococcus aureus cell viable – bacteria are excellent model systems to identify the entire protein assembly of a living organism. Many of the studies on physiological proteomics of bacteria, however, still rely on 2D gel-based technologies that visualize only a minor part of the proteome. more...

Organism:

Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus USA300; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus subsp. aureus COL

Type:

Expression profiling by array

Platform:

GPL7137

4 Samples

Download data: TXT