GEO DataSets

(Submitter supplied) Lyme disease spirochetes must induce RpoS-dependent genes during tick feeding to prepare for host infection. Previous work in our lab identified bbd18 as a negative regulator of RpoS, but inactivation of bbd18 in wild-type spirochetes was never achieved. In the current study we generated an inducible bbd18 gene at the endogenous plasmid locus and demonstrated the essential nature of BBD18 for viability of wild-type spirochetes in vitro and at a unique point in vivo. more...

Organism:

Borreliella burgdorferi B31

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32406

24 Samples

Download data: TXT

(Submitter supplied) Bacterial sepsis is a major killer in hospitalized patients. Coagulase-negative staphylococci (CNS) with the leading species Staphylococcus epidermidis are the most frequent causes of nosocomial sepsis, with most infectious isolates being methicillin resistant. However, which bacterial factors underlie the pathogenesis of CNS sepsis is unknown. While it has been commonly believed that invariant structures on the surface of CNS trigger sepsis by causing an over-reaction of the immune system, we show here that sepsis caused my methicillin-resistant S. more...

Organism:

Staphylococcus epidermidis; Staphylococcus aureus subsp. aureus MW2; Granulibacter bethesdensis; Chlamydia pneumoniae AR39; Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Chlamydia caviae GPIC; Staphylococcus haemolyticus JCSC1435; Coxiella burnetii; Rickettsia rickettsii; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Staphylococcus epidermidis ATCC 12228; Chlamydia trachomatis D/UW-3/CX

Type:

Expression profiling by array

Platform:

GPL4692

15 Samples

Download data: CEL, CHP

(Submitter supplied) Virulence of many bacterial pathogens, including the important human pathogen Staphylococcus aureus, depends on the secretion of frequently high amounts of toxins. Toxin production involves the need for the bacteria to make physiological adjustments for energy conservation. While toxins are primarily known to be targets of gene regulation, such changes may be accomplished by regulatory functions of the toxins themselves. more...

Organism:

Chlamydia pneumoniae AR39; Coxiella burnetii RSA 493; Granulibacter bethesdensis; Staphylococcus epidermidis ATCC 12228; Staphylococcus aureus subsp. aureus MW2; Borreliella burgdorferi B31; Chlamydia trachomatis D/UW-3/CX; Staphylococcus haemolyticus JCSC1435; Coxiella burnetii; Rickettsia rickettsii; Staphylococcus aureus; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Chlamydia caviae GPIC

Type:

Expression profiling by array

Platform:

GPL4692

12 Samples

Download data: CEL, CHP

(Submitter supplied) A hallmark of Coxiella burnetii, the bacterial cause of human Q fever, is a biphasic developmental cycle that generates biologically, ultrastructurally, and compositionally distinct large cell variant (LCV) and small cell variant (SCV) forms. LCVs are replicating, exponential phase forms while SCVs are non-replicating, stationary phase forms. The SCV has several properties, such as a condensed nucleoid and an unusual cell envelope, suspected of conferring enhanced environmental stability. more...

Organism:

Rickettsia rickettsii; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Chlamydia caviae GPIC; Staphylococcus haemolyticus JCSC1435; Staphylococcus epidermidis ATCC 12228; Staphylococcus aureus subsp. aureus MW2; Granulibacter bethesdensis; Coxiella burnetii; Chlamydia trachomatis D/UW-3/CX; Chlamydia pneumoniae AR39; Borreliella burgdorferi B31; Coxiella burnetii RSA 493

Type:

Expression profiling by array

Platform:

GPL4692

20 Samples

Download data: CEL, CHP

(Submitter supplied) Polymorphonuclear leukocytes (PMN) from patients with chronic granulomatous disease (CGD) fail to produce microbicidal concentrations of reactive oxygen species due to mutations in NOX2. Patients with CGD suffer from severe, life-threatening infections and inflammatory complications. Granulibacter bethesdensis is an emerging Gram-negative pathogen in CGD that resists killing by CGD PMN and inhibits PMN apoptosis through unknown mechanisms. more...

Organism:

Chlamydia pneumoniae AR39; Granulibacter bethesdensis; Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Chlamydia trachomatis D/UW-3/CX; Staphylococcus haemolyticus JCSC1435; Homo sapiens; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Staphylococcus aureus subsp. aureus MW2; Coxiella burnetii; Rickettsia rickettsii; Staphylococcus epidermidis ATCC 12228; Chlamydia caviae GPIC

Type:

Expression profiling by array

Platforms:

GPL4692 GPL570

123 Samples

Download data: CEL, CHP

(Submitter supplied) Transcriptional profiling of gene expression between parental strain B31 and rrp1 mutant. Cyclic-di-GMP is a bacterial second messenger that modulates many biological processes. Although its role in bacterial pathogenesis during mammalian infection has been widely recognized, the role of c-di-GMP in pathogen's life cycle in vector hosts is less understood. The enzootic cycle of the Lyme disease pathogen Borrelia burgdorferi involves both a mammalian host and an Ixodes tick vector. more...

Organism:

Borreliella burgdorferi; Borreliella burgdorferi B31

Type:

Expression profiling by array

Platform:

GPL6778

6 Samples

Download data: GPR

(Submitter supplied) Coxiella burnetii undergoes a biphasic developmental cycle within its host cell that generates morphologically and physiologically distinct large cell variants (LCV) and small cell variants (SCV). During the lag phase of the C. burnetii growth cycle, non-replicating SCV differentiate into replicating LCV that in turn differentiate back into SCV during stationary phase. Nearly homogeneous SCV are observed in infected Vero cells after extended incubation (21 to 28 days). more...

Organism:

Chlamydia pneumoniae AR39; Coxiella burnetii RSA 493; Granulibacter bethesdensis; Borreliella burgdorferi B31; Chlamydia trachomatis D/UW-3/CX; Staphylococcus haemolyticus JCSC1435; Staphylococcus epidermidis ATCC 12228; Staphylococcus aureus subsp. aureus MW2; Coxiella burnetii; Rickettsia rickettsii; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Chlamydia caviae GPIC

Type:

Expression profiling by array

Platform:

GPL4692

8 Samples

Download data: CEL, CHP

(Submitter supplied) Chlamydia trachomatis causes chronic inflammatory diseases of the eye and genital tract of global medical importance. The chlamydial plasmid plays an important role in the pathophysiology of these diseases as plasmid-deficient organisms are highly attenuated. The plasmid encodes both noncoding RNAs and eight conserved ORFs of undefined function. To understand plasmid gene function we generated plasmid shuttle vectors with deletions in each of the eight ORFs. more...

Organism:

Chlamydia muridarum; Staphylococcus epidermidis RP62A; Staphylococcus aureus subsp. aureus MW2; Staphylococcus haemolyticus JCSC1435; Chlamydia pneumoniae AR39; Staphylococcus epidermidis ATCC 12228; Chlamydia caviae GPIC; Granulibacter bethesdensis; Coxiella burnetii; Rickettsia rickettsii; Chlamydia trachomatis; Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Chlamydia trachomatis D/UW-3/CX

Type:

Expression profiling by array

Platform:

GPL4692

36 Samples

Download data: CEL, CHP

(Submitter supplied) Our study is the first to demonstrate BadR as a repressor of rpoS and thus facilitates spirochete’s transition back into ticks. BadR binds upstream and represses rpoS in unfed ticks. GLcNA-6P, an abundant nutrient of blood and released by subsequent remodeling of the tick peritrophic membrane, relieves repression of BadR on rpoS facilitating vertebrate host adaptation. BadR regulates chitobiose utilization genes and activates genes critical for spirochetes residence of ticks (lp28-4 genes) badR-deficient strain (Gene BB0693) compared to B31-A3 parental wild-type B. more...

Organism:

Borreliella burgdorferi B31

Type:

Expression profiling by array

Platform:

GPL15709

8 Samples

Download data: CALLS, PAIR

(Submitter supplied) Chlamydia trachomatis is a significant human pathogen yet their obligate intracellular nature severe restrictions upon research. Chlamydiae undergo a complex developmental cycle characterized by an infectious cell type known as the elementary body (EB) and an intracellular active replicative form called the reticulate body (RB). EBs have historically been described as metabolically dormant. A cell-free (axenic) culture system was developed which showed high levels of metabolic and biosynthetic activity from both EBs and RBs. more...

Organism:

Coxiella burnetii; Rickettsia rickettsii; Staphylococcus epidermidis ATCC 12228; Chlamydia trachomatis; Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Chlamydia caviae GPIC; Chlamydia trachomatis D/UW-3/CX; Staphylococcus haemolyticus JCSC1435; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Chlamydia pneumoniae AR39; Staphylococcus aureus subsp. aureus MW2; Granulibacter bethesdensis

Type:

Expression profiling by array

Platform:

GPL4692

20 Samples

Download data: CEL, CHP

(Submitter supplied) Methicillin-resistant Staphylococcus aureus (MRSA) is problematic both in hospitals and the community. Currently, we have limited understanding of mechanisms of innate immune evasion used by S. aureus. To that end, we created an isogenic deletion mutant in strain MW2 (USA400) of the saeR/S two-component gene regulatory system and studied its role in mouse models of pathogenesis and during human neutrophil interaction. more...

Organism:

Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Chlamydia trachomatis D/UW-3/CX; Chlamydia pneumoniae AR39; Staphylococcus epidermidis ATCC 12228; Staphylococcus aureus subsp. aureus MW2; Granulibacter bethesdensis; Coxiella burnetii; Rickettsia rickettsii; Staphylococcus aureus; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Chlamydia caviae GPIC; Staphylococcus haemolyticus JCSC1435

Type:

Expression profiling by array

Platform:

GPL4692

12 Samples

Download data: CEL, CHP

(Submitter supplied) RNA was isolated from late-log pahse wild-type Borrelia burgdorferi B31 and the bb0647 mutant grown in BSKH media at 37 degree, 5% CO2. cDNA was synthesized, labeled and hybridized to the 70mer oligonucleotide B. burgdorferi array. Slides were scanned using axon scanner and image were analyzed using GenePix 6.0. Dta were further analysed using the professional software Acuity 4.o, based on a ratio-based normalization.

Organism:

Borreliella burgdorferi B31; Borreliella burgdorferi

Type:

Expression profiling by array

Platform:

GPL6778

5 Samples

Download data: GPR

(Submitter supplied) Rickettsiae are strict obligate intracellular pathogens that alternate between arthropod and mammalian hosts in a zoonotic cycle. Typically, pathogenic bacteria that cycle between environmental sources and mammalian hosts adapt to the respective environments by coordinately regulating gene expression such that genes essential for survival and virulence are expressed only upon infection of mammals. Temperature is a common environmental signal for upregulation of virulence gene expression although other factors may also play a role. more...

Organism:

Staphylococcus epidermidis ATCC 12228; Borreliella burgdorferi B31; Chlamydia trachomatis D/UW-3/CX; Chlamydia pneumoniae AR39; Coxiella burnetii RSA 493; Chlamydia caviae GPIC; Granulibacter bethesdensis; Coxiella burnetii; Rickettsia rickettsii; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Staphylococcus aureus subsp. aureus MW2; Staphylococcus haemolyticus JCSC1435

Type:

Expression profiling by array

Platform:

GPL4692

97 Samples

Download data: CEL, CHP

(Submitter supplied) C. trachomatis possess a cryptic 7.5 kb plasmid of unknown function. Here we describe a comprehensive molecular and biological characterization of the naturally occurring plasmidless human Chlamydia trachomatis strain L2 (25667R). We found that despite minimal chromosomal polymorphisms the LGV L2 (25667R) strain was indistinguishable from the L2 (434) plasmid positive strain in its in vitro infectivity characteristics such as growth kinetics, plaquing efficiency, and plaque size. more...

Organism:

Coxiella burnetii; Rickettsia rickettsii; Staphylococcus epidermidis ATCC 12228; Chlamydia caviae GPIC; Chlamydia trachomatis; Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Chlamydia trachomatis D/UW-3/CX; Staphylococcus haemolyticus JCSC1435; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Staphylococcus aureus subsp. aureus MW2; Chlamydia pneumoniae AR39; Granulibacter bethesdensis

Type:

Expression profiling by array

Platform:

GPL4692

18 Samples

Download data: CEL, CHP

(Submitter supplied) The Staphylococcus aureus accessory gene regulator (agr) is a prototype quorum-sensing system in Gram-positive bacteria and a paradigmatic example of gene regulation by a small regulatory RNA, RNAIII. Using genome-wide transcriptional profiling in the community-associated methicillin-resistant (CA-MRSA) strain MW2, we demonstrate here that in contrast to the current model of target gene regulation by agr, a large subset of agr-regulated genes is controlled independently of RNAIII. more...

Organism:

Coxiella burnetii; Rickettsia rickettsii; Staphylococcus aureus; Staphylococcus epidermidis ATCC 12228; Chlamydia caviae GPIC; Borreliella burgdorferi B31; Chlamydia trachomatis D/UW-3/CX; Staphylococcus haemolyticus JCSC1435; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Staphylococcus aureus subsp. aureus MW2; Chlamydia pneumoniae AR39; Coxiella burnetii RSA 493; Granulibacter bethesdensis

Type:

Expression profiling by array

Platform:

GPL4692

27 Samples

Download data: CEL, CHP

(Submitter supplied) Autoinducer 2 (AI-2), a widespread by-product of the LuxS-catalyzed S-ribosylhomocysteine cleavage reaction in the activated methyl cycle, has been suggested to serve as an intra- and interspecies signaling molecule, but in many bacteria AI-2 control of gene expression is not completely understood. Particularly, we have a lack of knowledge about AI-2 signaling in the important human pathogens Staphylococcus aureus and S. more...

Organism:

Staphylococcus epidermidis; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Chlamydia caviae GPIC; Staphylococcus haemolyticus JCSC1435; Coxiella burnetii; Rickettsia rickettsii; Staphylococcus epidermidis ATCC 12228; Staphylococcus aureus subsp. aureus MW2; Granulibacter bethesdensis; Chlamydia pneumoniae AR39; Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Chlamydia trachomatis D/UW-3/CX

Type:

Expression profiling by array

Platform:

GPL4692

9 Samples

Download data: CEL, CHP

(Submitter supplied) Panton-valentine leukocidin (PVL) has been linked to worldwide emergence of community-associated methicillin resistant Staphylococcus aureus (CA-MRSA) -- its role in virulence in unclear. Here we show that PVL had no effect on global gene expression of prominent CA-MRSA strains nor did it affect bacterial clearance from lungs, spleen and kidneys in a highly discriminatory rabbit bacteremia model. These findings negate a large body of epidemiological research that implicated PVL in CA-MRSA virulence. more...

Organism:

Coxiella burnetii; Rickettsia rickettsii; Staphylococcus aureus; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Chlamydia caviae GPIC; Chlamydia trachomatis; Borreliella burgdorferi B31; Chlamydia trachomatis D/UW-3/CX; Staphylococcus haemolyticus JCSC1435; Streptococcus pyogenes; Staphylococcus epidermidis ATCC 12228; Staphylococcus aureus subsp. aureus MW2; Borreliella burgdorferi; Yersinia pestis; Chlamydia pneumoniae AR39; Coxiella burnetii RSA 493; Granulibacter bethesdensis

Type:

Expression profiling by array

Platforms:

GPL2129 GPL4692

30 Samples

Download data: CEL, CHP

(Submitter supplied) The RpoN-RpoS alternative sigma factor pathway is essential for key adaptive responses by Borrelia burgdorferi, particularly those involved in the infection of a mammalian host. A putative response regulator, Rrp2, ostensibly is required for activation of the RpoN-dependent transcription of rpoS. However, questions remain regarding the extent to which the three major constituents of this pathway (Rrp2, RpoN, and RpoS) act interdependently. more...

Organism:

Borreliella burgdorferi B31; Borreliella burgdorferi

Type:

Expression profiling by array

Platform:

GPL6778

22 Samples

Download data: GPR

(Submitter supplied) Biofilm-associated infection by the leading nosocomial pathogen Staphylococcus epidermidis is a major problem for the public health system. Here we used an especially discriminatory, two-step screen to discover key biofilm factors. We identified the transcriptional regulator and SarA paralog SarZ as a novel important determinant of biofilm formation and biofilm-associated infection by S. epidermidis. more...

Organism:

Staphylococcus epidermidis; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Staphylococcus aureus subsp. aureus MW2; Staphylococcus haemolyticus JCSC1435; Coxiella burnetii; Rickettsia rickettsii; Chlamydia pneumoniae AR39; Staphylococcus epidermidis ATCC 12228; Coxiella burnetii RSA 493; Chlamydia caviae GPIC; Granulibacter bethesdensis; Borreliella burgdorferi B31; Chlamydia trachomatis D/UW-3/CX

Type:

Expression profiling by array

Dataset:

GDS3426

Platform:

GPL4692

6 Samples

Download data: CEL, CHP

Analysis of Staphylococcus epidermidis sarZ mutants deficient in biofilm formation. Biofilm-associated infections due to Staphylococcus epidermidis, a nosocomial pathogen, is a public health problem. Results provide insight into the role of SarZ in biofilm formation and virulence in S. epidermidis.

Organism:

Coxiella burnetii; Rickettsia rickettsii; Staphylococcus epidermidis; Chlamydia muridarum; Chlamydophila pneumoniae AR39; Staphylococcus epidermidis RP62A; Staphylococcus epidermidis ATCC 12228; Staphylococcus aureus subsp. aureus MW2; Borrelia burgdorferi B31; Coxiella burnetii RSA 493; Chlamydophila caviae GPIC; Chlamydia trachomatis D/UW-3/CX; Staphylococcus haemolyticus JCSC1435; Granulibacter bethesdensis

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL4692

Series:

GSE8523

6 Samples

Download data: CEL, CHP