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Items: 19

1.

RNA at 92 °C: the non-coding transcriptome of the hyperthermophilic archaeon Pyrococcus abyssi.

(Submitter supplied) The non-coding transcriptome of the hyperthermophilic archaeon Pyrococcus abyssi is investigated using the RNA-seq technology. A dedicated computational pipeline analyzes RNA-seq reads and prior genome annotation to identify small RNAs, untranslated regions of mRNAs, and cis-encoded antisense transcripts. Unlike other archaea, such as Sulfolobus and Halobacteriales, P. abyssi produces few leaderless mRNA transcripts. more...
Organism:
Pyrococcus abyssi
Type:
Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing
Platform:
GPL18745
1 Sample
Download data: GFF
Series
Accession:
GSE58131
ID:
200058131
2.

Origin of replication for Pyrococcus abyssi by ChIP-chip

(Submitter supplied) Recent advances in the study of archaeal DNA replication have uncovered defined replication origins (oriC) and demonstrated specific binding of the Cdc6/Orc1 protein and Mcm helicase to oriC in vivo and/or in vitro. The oriC of the hyperthermophilic archaeon Pyrococcus abyssi is characterized by 13 bp repeats, AT-rich regions and an inverted repeat whose precise roles remain unclear. We report here that the 13 bp repeats are widespread in the three Pyrococcus genomes. more...
Organism:
Pyrococcus abyssi
Type:
Genome binding/occupancy profiling by genome tiling array
Platform:
GPL3384
14 Samples
Download data: PDF, TXT
Series
Accession:
GSE4104
ID:
200004104
3.

Illumina Genome Analyzer IIx (Pyrococcus abyssi)

Organism:
Pyrococcus abyssi
1 Series
1 Sample
Download data
Platform
Accession:
GPL18745
ID:
100018745
4.

Origin of replication for Pyrococcus abyssi by ChIP-chip

(Submitter supplied) Chromatin immunopreciptation coupled with hybridization on a whole genome microarray (ChIP-chip) of P. abyssi. Protocol: Microarray preparation. The P. abyssi genome was divided into 353 regions, each of which is 5 kb in length. Each of the 353 DNA regions has a specific ID number. For example, the microarray region 25 corresponds to the genome coordinates 120 000 to 125 000. For the microarrays, each of the 353 regions was amplified with the BD Advantage 2 PCR kit (BD biosciences clontech). more...
Organism:
Pyrococcus abyssi
1 Series
14 Samples
Download data
Platform
Accession:
GPL3384
ID:
100003384
5.

Pabyssi_RNAseq

Organism:
Pyrococcus abyssi
Source name:
cells
Platform:
GPL18745
Series:
GSE58131
Download data: GFF
Sample
Accession:
GSM1401488
ID:
301401488
6.

Mcm/ exponential phase/ replica 4

Organism:
Pyrococcus abyssi
Source name:
ChIP co-immunoprecipitated DNA using Mcm antibody and archaebacteria in exponential phase, fourth replica (channel 1) DNA from mock-treated cell extract and archaebacteria treated in exponential phase, fourth replica (channel 2)
Platform:
GPL3384
Series:
GSE4104
Download data
Sample
Accession:
GSM93628
ID:
300093628
7.

Mcm/ exponential phase/ replica 2

Organism:
Pyrococcus abyssi
Source name:
ChIP co-immunoprecipitated DNA using Mcm antibody and archaebacteria in exponential phase, second replica (channel 1) DNA from mock-treated cell extract and archaebacteria treated in exponential phase, second replica (channel 2)
Platform:
GPL3384
Series:
GSE4104
Download data
Sample
Accession:
GSM93627
ID:
300093627
8.

Mcm/ exponential phase/ replica 1

Organism:
Pyrococcus abyssi
Source name:
ChIP co-immunoprecipitated DNA using Mcm antibody and archaebacteria in exponential phase, first replica (channel 1) DNA from mock-treated cell extract and archaebacteria treated in exponential phase, first replica (channel 2)
Platform:
GPL3384
Series:
GSE4104
Download data
Sample
Accession:
GSM93626
ID:
300093626
9.

Mcm/ stationary phase/ replica 4

Organism:
Pyrococcus abyssi
Source name:
ChIP co-immunoprecipitated DNA using Mcm antibody and archaebacteria in stationary phase, fourth replica (channel 1) DNA from mock-treated cell extract and archaebacteria treated in stationary phase, fourth replica (channel 2)
Platform:
GPL3384
Series:
GSE4104
Download data
Sample
Accession:
GSM93625
ID:
300093625
10.

Mcm/ stationary phase/ replica 3

Organism:
Pyrococcus abyssi
Source name:
ChIP co-immunoprecipitated DNA using Mcm antibody and archaebacteria in stationary phase, third replica (channel 1) DNA from mock-treated cell extract and archaebacteria treated in exponential phase, third replica (channel 2)
Platform:
GPL3384
Series:
GSE4104
Download data
Sample
Accession:
GSM93624
ID:
300093624
11.

Mcm/ stationary phase/ replica 2

Organism:
Pyrococcus abyssi
Source name:
ChIP co-immunoprecipitated DNA using Mcm antibody and archaebacteria in stationary phase, second replica (channel 1) DNA from mock-treated cell extract and archaebacteria treated in stationary phase, second replica (channel 2)
Platform:
GPL3384
Series:
GSE4104
Download data
Sample
Accession:
GSM93623
ID:
300093623
12.

Mcm/ stationary phase/ replica 1

Organism:
Pyrococcus abyssi
Source name:
ChIP co-immunoprecipitated DNA using Mcm antibody and archaebacteria in stationary phase, first replica (channel 1) DNA from mock-treated cell extract and archaebacteria treated in stationary phase, first replica (channel 2)
Platform:
GPL3384
Series:
GSE4104
Download data
Sample
Accession:
GSM93622
ID:
300093622
13.

Cdc 6/exponential phase replica 4

Organism:
Pyrococcus abyssi
Source name:
ChIP co-immunoprecipitated DNA using Cdc 6 antibody and archaebacteria in exponential phase, fourth replica (channel 1) DNA from mock-treated cell extract and archaebacteria treated in exponential phase, fourth replica (channel 2)
Platform:
GPL3384
Series:
GSE4104
Download data
Sample
Accession:
GSM93621
ID:
300093621
14.

Cdc 6/exponential phase replica 2

Organism:
Pyrococcus abyssi
Source name:
ChIP co-immunoprecipitated DNA using Cdc 6 antibody and archaebacteria in exponential phase, second replica (channel 1) DNA from mock-treated cell extract and archaebacteria treated in exponential phase, second replica (channel 2)
Platform:
GPL3384
Series:
GSE4104
Download data
Sample
Accession:
GSM93620
ID:
300093620
15.

Cdc 6/exponential phase replica 1

Organism:
Pyrococcus abyssi
Source name:
ChIP co-immunoprecipitated DNA using Cdc 6 antibody and archaebacteria in exponential phase, first replica (channel 1) DNA from mock-treated cell extract and archaebacteria treated in exponential phase, first replica (channel 2)
Platform:
GPL3384
Series:
GSE4104
Download data
Sample
Accession:
GSM93619
ID:
300093619
16.

Cdc 6/stationary phase replica 4

Organism:
Pyrococcus abyssi
Source name:
ChIP co-immunoprecipitated DNA using Cdc 6 antibody and archaebacteria in stationary phase, fourth replica (channel 1) DNA from mock-treated cell extract and archaebacteria treated in stationary phase, fourth replica (channel 2)
Platform:
GPL3384
Series:
GSE4104
Download data
Sample
Accession:
GSM93618
ID:
300093618
17.

Cdc 6/stationary phase replica 2

Organism:
Pyrococcus abyssi
Source name:
ChIP co-immunoprecipitated DNA using Cdc 6 antibody and archaebacteria in stationary phase, second replica (channel 1) DNA from mock-treated cell extract and archaebacteria treated in stationary phase, second replica (channel 2)
Platform:
GPL3384
Series:
GSE4104
Download data
Sample
Accession:
GSM93617
ID:
300093617
18.

Cdc 6/stationary phase

Organism:
Pyrococcus abyssi
Source name:
ChIP co-immunoprecipitated DNA using Cdc 6 antibody and archaebacteria in stationary phase, first replica (channel 1) DNA from mock-treated cell extract and archaebacteria treated in stationary phase, first replica (channel 2)
Platform:
GPL3384
Series:
GSE4104
Download data
Sample
Accession:
GSM93616
ID:
300093616
19.

Cdc 6/puromycin

Organism:
Pyrococcus abyssi
Source name:
ChIP co-immunoprecipitated DNA using Cdc 6 antibody and archaebacteria treated with puromycin, fourth replica (channel 1) DNA from mock-treated cell extract and archaebacteria treated with puromycin, fourth replica (channel 2)
Platform:
GPL3384
Series:
GSE4104
Download data
Sample
Accession:
GSM93565
ID:
300093565
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