GEO DataSets

(Submitter supplied) Cyanobacteria frequently constitute integral components of microbial communities known as phototrophic biofilms, which are widespread in various environments and hold significant industrial relevance. Previous studies of the model cyanobacterium Synechococcus elongatus PCC 7942 revealed that its planktonic growth habit results from a biofilm-suppression mechanism that depends on an extracellular inhibitor, an observation that opens the door to investigating cyanobacterial intercellular communication. more...

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Expression profiling by high throughput sequencing

Platform:

GPL34136

32 Samples

Download data: XLSX

(Submitter supplied) Photoperiodic Time Measurement (PPTM) is the ability of plants and animals to measure differences in day/night-length (photoperiod, PP) and use that information to anticipate seasonal changes in key environmental factors such as annual changes in average temperature. This timekeeping phenomenon, which is well documented for higher organisms, enables processes such as gonadal growth/regression, flowering, hibernation, and diapause to optimally adapt to annual transformations of the environment. more...

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32996

36 Samples

Download data: CSV

(Submitter supplied) Cyanobacteria are attractive hosts for producing pharmaceuticals, renewable fuels, and chemicals due to their ability to use sunlight as their energy source. Despite the application of traditional genetic tools such as the identification of strong promoters to enhance the expression of heterologous genes, however, cyanobacteria have lagged behind other microorganisms such as E.coli and yeast as economically efficient bioreactors. more...

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32996

12 Samples

Download data: CSV

(Submitter supplied) Regulation of CO2 fixation in cyanobacteria is important both for the organism and the global carbon balance. Here we show that phosphoketolase in Synechococcus elongatus PCC7942 (SeXPK) possesses a distinct ATP sensing mechanism, which upon ATP drops, allows SeXPK to divert precursors of the RuBisCO substrate away from the Calvin-Benson-Bassham (CBB) cycle. Deleting the SeXPK gene increased CO2 fixation particularly during light-dark transitions. more...

Organism:

Synechococcus elongatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33256

24 Samples

Download data: TXT

(Submitter supplied) Here, we treated the model cyanobacterium Synechococcus elongatus PCC7942 with streptomycin, which selectively binds to bacterial ribosomes, at a concentration of 50 µg/mL and performed transcriptome analysis using RNA sequencing. In addition to antibiotic stress, we treated salt stress (0.4 M NaCl) and performed RNA sequencing analysis.

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32996

10 Samples

Download data: XLSX

(Submitter supplied) In order to explore the effect of introducing the ectoine synthesis module in Synechococcus elongatus PCC 7942 on the global regulation of the strain under high-salt environment. The ectoine synthesis module was introduced at the Synpcc7942_0808 locus to obtain the Syn7942/Δsps-ect strain that lacked sucrose synthesis and could synthesize ectoine. Comparative transcriptomic analysis was performed on WT and Syn7942/Δsps-ect under 0 mM and 300 mM NaCl conditions.

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32996

12 Samples

Download data: XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL32315

46 Samples

Download data: TXT

(Submitter supplied) Biofilm formation by photosynthetic organisms is a complex behavior that serves multiple functions in the environment. Biofilm formation in the unicellular cyanobacterium Synechococcus elongatus PCC 7942 is regulated in part by a set of small secreted proteins that promotes biofilm formation and a self-suppression mechanism that prevents their expression. Little is known about the regulatory and structural components of the biofilms in PCC 7942, or response to the suppressor signal(s). more...

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32315

21 Samples

Download data: TXT

(Submitter supplied) Biofilm formation by photosynthetic organisms is a complex behavior that serves multiple functions in the environment. Biofilm formation in the unicellular cyanobacterium Synechococcus elongatus PCC 7942 is regulated in part by a set of small secreted proteins that promotes biofilm formation and a self-suppression mechanism that prevents their expression. Little is known about the regulatory and structural components of the biofilms in PCC 7942, or response to the suppressor signal(s). more...

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Other

Platform:

GPL32315

25 Samples

Download data: TXT

(Submitter supplied) An excess of reactive oxygen species (ROS) can cause severe oxidative damage to cellular components in photosynthetic cells. Antioxidant systems, such as the ascorbate-glutathione cycle, regulate redox status in cells to guard against such damage. Dehydroascorbate reductase (DHAR, EC 1.8.5.1) catalyzes the glutathione-dependent reduction of oxidized ascorbate (dehydroascorbate) and contains a redox active site and glutathione binding-site. more...

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17750

4 Samples

Download data: TXT

(Submitter supplied) Zinc oxide (ZnO) nanoparticles are commonly used in sunscreens for their UV-filtering properties. Their growing use can lead to their release into ecosystems, raising question about their toxicity. Effects of these engineered nanomaterials (ENMs) on cyanobacteria, which are important primary producers involved in many biogeochemical cycles, are unknown. In this study, we investigated by several complementary approaches the toxicological effects of two marketed ZnO-ENMs (coated and uncoated) on the model cyanobacteria Synechococcus elongatus PCC 7942. more...

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Expression profiling by array

Platform:

GPL9534

16 Samples

Download data: TXT

(Submitter supplied) The circadian clock of the cyanobacterium Synechococcus elongatus PCC 7942 drives oscillations in global mRNA abundances with 24 hour periodicity under constant light conditions. The transcription factor RpaA, a circadian clock-regulated transcription factor, controls the timing of circadian gene expression, but the mechanisms underlying this control are not well understood. Here we show that four RpaA-dependent sigma factors – RpoD2, RpoD6, RpoD5, and SigF2 – are sequentially activated downstream of active RpaA and are required for proper expression of circadian mRNAs. more...

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL16957

78 Samples

Download data: XLSX

(Submitter supplied) The goals of this study are to compare hydrogen peroxide (H2O2) stress tolerance through gene expression of wild type (WT) and OsTPX-expressing Synechoccous elongatus PCC 7942 (OT) under the normal and stress condition.

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17750

4 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL17750

97 Samples

Download data

(Submitter supplied) We used RNA sequencing to measure genome-wide gene expression in the cyanobacterium Synechococcus elongatus PCC 7942 grown under dynamic light regimes that mimic the variation in light intensity seen on a Clear Day in nature, or the rapid changes in light intensity that accompany changes in shading We compare these gene expression dynamics to those of a culture grown under a Low Light condition that mimics the standard laboratory conditions used for study of cyanobacteria. more...

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17750

60 Samples

Download data: XLSX

(Submitter supplied) We used ChIP sequencing to measure genome-wide binding of transcription factors in the cyanobacterium Synechococcus elongatus PCC 7942 grown under dynamic light regimes that mimic the rapid changes in light intensity that accompany changes in shading. Our analysis reveals that rapid changes in light intensity modulate the binding of RNA polymerase (RNAP) upstream of genes in a way that correlates with changes in downstream gene expression, suggesting that changes in transcriptional regulation control light-responsive gene expression changes. more...

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17750

37 Samples

Download data: XLSX

(Submitter supplied) We investigated the role of ppGpp in Synechococcus elongatus PCC7942 in light/dark conditions. We performed ChIP-seq experiments using wild type and the rel- strain at dusk, during exposure to 12 h of darkness and at dawn. We show that the RNA polymerase pausing index is globally lower in the rel- strain.

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL16957

12 Samples

Download data: XLSX

(Submitter supplied) We investigated the role of basal ppGpp levels in Synechococcus elongatus PCC7942 in adaptation to darkness. We performed RNA-seq experiments using the rel- relA+ and the rel- relAE335Q strain at dusk and during exposure to 12 h of darkness. We show that basal ppGpp levels is not sufficient for restoration of appropriate transcriptional shutdown in response to darkness.

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16957

36 Samples

Download data: XLS

(Submitter supplied) We investigated the role of ppGpp in Synechococcus elongatus PCC7942 in light/dark conditions. We performed RNA-seq experiments using wild type and the rel- strain at dusk and during exposure to 12 h of darkness. We show that ppGpp is required for correct response to darkness.

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16957

36 Samples

Download data: XLS

(Submitter supplied) We investigated the role of ppGpp in Synechococcus elongatus PCC7942 in constant light. We performed RNA-seq experiments using wild type + relA+ and wild type + relAE335Q strains after induction with theophylline starting at subjective dusk. We show that ppGpp induction inhibits global transcription in light.

Organism:

Synechococcus elongatus PCC 7942 = FACHB-805

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16957

6 Samples

Download data: XLS