Release Notes For GenBank Release 120
GBREL.TXT Genetic Sequence Data Bank
October 15 2000
NCBI-GenBank Flat File Release 120.0
Distribution Release Notes
9102634 loci, 10335692655 bases, from 9102634 reported sequences
This document describes the format and content of the flat files that
comprise releases of the GenBank database. If you have any questions or
comments about GenBank or this document, please contact NCBI via email
at [email protected] or:
GenBank
National Center for Biotechnology Information
National Library of Medicine, 38A, 8N805
8600 Rockville Pike
Bethesda, MD 20894
USA
Phone: (301) 496-2475
Fax: (301) 480-9241
==========================================================================
TABLE OF CONTENTS
==========================================================================
1. INTRODUCTION
1.1 Release 120.0
1.2 Cutoff Date
1.3 Important Changes in Release 120.0
1.4 Upcoming Changes
1.5 Request for Direct Submission of Sequence Data
1.6 Organization of This Document
2. ORGANIZATION OF DATA FILES
2.1 Overview
2.2 Files
2.2.1 File Descriptions
2.2.5 File Sizes
2.2.6 Per-Division Statistics
2.2.7 Selected Per-Organism Statistics
2.2.8 Growth of GenBank
3. FILE FORMATS
3.1 File Header Information
3.2 Directory Files
3.2.1 Short Directory File
3.3 Index Files
3.3.1 Accession Number Index File
3.3.2 Keyword Phrase Index File
3.3.3 Author Name Index File
3.3.4 Journal Citation Index File
3.3.5 Gene Name Index
3.4 Sequence Entry Files
3.4.1 File Organization
3.4.2 Entry Organization
3.4.3 Sample Sequence Data File
3.4.4 LOCUS Format
3.4.5 DEFINITION Format
3.4.5.1 DEFINITION Format for NLM Entries
3.4.6 ACCESSION Format
3.4.7 VERSION Format
3.4.8 KEYWORDS Format
3.4.9 SEGMENT Format
3.4.10 SOURCE Format
3.4.11 REFERENCE Format
3.4.12 FEATURES Format
3.4.12.1 Feature Key Names
3.4.12.2 Feature Location
3.4.12.3 Feature Qualifiers
3.4.12.4 Cross-Reference Information
3.4.12.5 Feature Table Examples
3.4.13 ORIGIN Format
3.4.14 SEQUENCE Format
4. ALTERNATE RELEASES
5. KNOWN PROBLEMS OF THE GENBANK DATABASE
5.1 Incorrect Gene Symbols in Entries and Index
6. GENBANK ADMINISTRATION
6.1 Registered Trademark Notice
6.2 Citing GenBank
6.3 GenBank Distribution Formats and Media
6.4 Other Methods of Accessing GenBank Data
6.5 Request for Corrections and Comments
6.6 Credits and Acknowledgments
6.7 Disclaimer
==========================================================================
1. INTRODUCTION
1.1 Release 120.0
The National Center for Biotechnology Information (NCBI) at the National
Library of Medicine (NLM), National Institutes of Health (NIH) is responsible
for producing and distributing the GenBank Sequence Database. NCBI handles
all GenBank direct submissions and authors are advised to use the address
below. Submitters are encouraged to use the free Sequin software package
for sending sequence data, or the newly developed World Wide Web submission
form. See Section 1.5 below for details.
*****************************************************************************
The address for direct submissions to GenBank is:
GenBank Submissions
National Center for Biotechnology Information
Bldg 38A, Rm. 8N-803
8600 Rockville Pike
Bethesda, MD 20894
E-MAIL: [email protected]
Updates and changes to existing GenBank records:
E-MAIL: [email protected]
URL for the new GenBank submission tool - BankIt - on the World Wide Web:
http://www.ncbi.nlm.nih.gov/
(see Section 1.5 for additional details about submitting data to GenBank.)
*****************************************************************************
GenBank Release 120.0 is a release of sequence data by NCBI in the GenBank
flat file format. GenBank is a component of a tri-partite, international
collaboration of sequence databases in the U.S., Europe, and Japan. The
collaborating databases in Europe are the European Molecular Biology Laboratory
(EMBL) at Hinxton Hall, UK, and the DNA Database of Japan (DDBJ) in Mishima,
Japan. Sequence data is also incorporated from the Genome Sequence Data Base
(GSDB), Santa Fe, NM. Patent sequences are incorporated through arrangements
with the U.S. Patent and Trademark Office, and via the collaborating
international databases from other international patent offices. The database
is converted to various output formats, including the Flat File and Abstract
Syntax Notation 1 (ASN.1) versions. The ASN.1 and Flat File forms of the data
are available at NCBI's anonymous FTP server: ncbi.nlm.nih.gov .
1.2 Cutoff Date
This full release, 120.0, incorporates data available to the databases as of
October 11, 2000. For more recent data, users are advised to:
o Download the GenBank Update files by anonymous FTP to 'ncbi.nlm.nih.gov':
ftp://ncbi.nlm.nih.gov/ncbi-asn1 (ASN.1 format)
ftp://ncbi.nlm.nih.gov/genbank (flatfile format)
o Use the Network-Entrez or Web-Entrez applications to interactively query
the Entrez: Nucleotides database (see Section 6.4 of this document).
o Use the NCBI 'query' email server to search the GenBank Updates. Instructions
regarding the use of the e-mail server can be obtained by sending an email
message with the word 'help' in it to: [email protected]
1.3 Important Changes in Release 120.0
1.3.1 Organizational changes
Due to database growth, the EST division is now being split into eighty-seven
pieces.
Due to database growth, the GSS division is now being split into twenty-eight
pieces.
Due to database growth, the HTG division is now being split into twenty-four
pieces.
Due to database growth, the PRI division is now being split into eight pieces.
Due to database growth, the PAT division is now being split into two pieces:
gbpat1.seq and gbpat2.seq .
1.3.2 Order of entries in the Short Directory file has changed
The gbsdr.txt file which accompanies GenBank releases contains the
DEFINITION line and number of bases for every sequence. For historical
reasons, a specific division ordering was used for the sections of this
file. This order began with:
PRIMATE
RODENT
OTHER MAMMALIAN
OTHER VERTEBRATE
INVERTEBRATE
....
and ended with:
SEQUENCE TAGGED SITE
GENOME SURVEY SEQUENCE
HIGH THROUGHPUT GENOMIC SEQUENCING
Starting with GenBank Release 120.0, the ordering of the sections
of this file is now determined solely by the sort-order of the
GenBank division codes. For example:
bct1
bct2
...
est1
...
gss1
...
htg1
...
vrl1
vrl2
vrt
1.3.3 The gbaut.idx file has been split into multiple pieces
The gbaut.idx file exceeded 3 GB in size for GenBank 119.0, so it has
been split into seven pieces of approximately 500 MB each:
gbaut1.idx
gbaut2.idx
gbaut3.idx
gbaut4.idx
gbaut5.idx
gbaut6.idx
gbaut7.idx
1.4 Upcoming Changes
1.4.1 NCBI's ftp address will be changed
At some point in the near future NCBI's ftp address will be changed.
The current address:
ncbi.nlm.nih.gov
will become:
ftp.ncbi.nih.gov
Additional details about this change will be made available via these
release notes and the GenBank newsgroup (bionet.molbio.genbank) as they
become available.
1.4.2 Selenocysteine representation
Selenocysteine residues within the protein translations of coding
region features have been represented in GenBank via the letter 'X'
and a /transl_except qualifier. At the May 1999 DDBJ/EMBL/GenBank
collaborative meeting, it was learned that IUPAC plans to adopt the
letter 'U' for selenocysteine.
DDBJ, EMBL, and GenBank will thus use this new amino acid abbreviation
for its /translation qualifiers. Although a timetable for its appearance
has not been finalized, we are mentioning this now because the introduction
of a new residue abbreviation is a fairly fundamental change.
Details about the use of 'U' will be made available via these release
notes and the GenBank newsgroup as they become available.
1.4.3 New REFERENCE type for on-line journals
Agreement was reached at the May 1999 collaborative DDBJ/EMBL/GenBank
meeting that an effort should be made to accomodate references which are
published only on-line. Until specifications for such references are
available from library organizations, GenBank will present them in a manner
like this:
REFERENCE 1 (bases 1 to 2858)
AUTHORS Smith, J.
TITLE Cloning and expression of a phospholipase gene
JOURNAL Online Publication
REMARK Online-Journal-name; Article Identifier; URL
This format is still tentative; additional information about this new
reference type will be made available via these release notes.
1.5 Request for Direct Submission of Sequence Data
A successful GenBank requires that the data enter the database as soon
as possible after publication, that the annotations be as complete as
possible, and that the sequence and annotation data be accurate. All
three of these requirements are best met if authors of sequence data
submit their data directly to GenBank in a usable form. It is especially
important that these submissions be in computer-readable form.
GenBank must rely on direct author submission of data to ensure that
it achieves its goals of completeness, accuracy, and timeliness. To
assist researchers in entering their own sequence data, GenBank
provides a WWW submission tool called BankIt, as well as a stand-alone
software package called Sequin. BankIt and Sequin are both easy-to-use
programs that enable authors to enter a sequence, annotate it, and
submit it to GenBank. Through the international collaboration of DNA
sequence databases, GenBank submissions are forwarded daily for inclusion
in the EMBL and DDBJ databases.
SEQUIN. Sequin is an interactive, graphically-oriented program based
on screen forms and controlled vocabularies that guides you through the
process of entering your sequence and providing biological and
bibliographic annotation. Sequin is designed to simplify the sequence submission
process, and to provide increased data handling capabilities to accomodate
very long sequences, complex annotations, and robust error checking. E-mail
the completed submission file to : [email protected]
Sequin is provided for Macintosh, PC/Windows, UNIX and VMS computers.
It is available by annonymous ftp from ncbi.nlm.nih.gov; login as
anonymous and use your e-mail address as the password. It is located in
the sequin directory. Or direct your web browser to this URL:
ftp://ncbi.nlm.nih.gov/sequin
BANKIT. BankIt provides a simple forms approach for submitting your
sequence and descriptive information to GenBank. Your submission will
be submitted directly to GenBank via the World Wide Web, and
immediately forwarded for inclusion in the EMBL and DDBJ databases.
BankIt may be used with Netscape, Internet Explorer, and other common
WWW clients. You can access BankIt from GenBank's home page:
http://www.ncbi.nlm.nih.gov/
AUTHORIN. Authorin sequence submissions are no longer accepted by
GenBank, and the Authorin application is no longer distributed by NCBI.
If you have questions about GenBank submissions or any of the data
submission tools, contact NCBI at: [email protected] or 301-496-2475.
1.6 Organization of This Document
The second section describes the contents of GenBank releases. The third
section illustrates the formats of the flat files. The fourth section
describes other versions of the data, the fifth section identifies known prob-
lems, and the sixth contains administrative details.
2. ORGANIZATION OF DATA FILES
2.1 Overview
GenBank releases consist of a set of ASCII text files, most of which
contain sequence data. A few supplemental "index" files are also supplied,
containing comprehensive lists of author names, journal citations,
gene names, and keywords, along with the accession numbers of the records
in which they can be found (see Section 3.3). The line-lengths of
these files is variable.
2.2 Files
This GenBank flat file release consists of 182 files. The list
that follows describes each of the files included in the distribution.
Their sizes and base pair content are also summarized.
2.2.1 File Descriptions
1. gbrel.txt - Release notes (this document).
2. gbsdr.txt - Short directory of the data bank.
3. gbacc.idx - Index of the entries according to accession number.
4. gbkey.idx - Index of the entries according to keyword phrase.
5. gbaut1.idx - Index of the entries according to author, part 1.
6. gbaut2.idx - Index of the entries according to author, part 2.
7. gbaut3.idx - Index of the entries according to author, part 3.
8. gbaut4.idx - Index of the entries according to author, part 4.
9. gbaut5.idx - Index of the entries according to author, part 5.
10. gbaut6.idx - Index of the entries according to author, part 6.
11. gbaut7.idx - Index of the entries according to author, part 7.
12. gbjou.idx - Index of the entries according to journal citation.
13. gbgen.idx - Index of the entries according to gene names.
14. gbsec.idx - Index of the entries according to secondary accession number.
15. gbpri1.seq - Primate sequence entries, part 1.
16. gbpri2.seq - Primate sequence entries, part 2.
17. gbpri3.seq - Primate sequence entries, part 3.
18. gbpri4.seq - Primate sequence entries, part 4.
19. gbpri5.seq - Primate sequence entries, part 5.
20. gbpri6.seq - Primate sequence entries, part 6.
21. gbpri7.seq - Primate sequence entries, part 7.
22. gbpri8.seq - Primate sequence entries, part 8.
23. gbrod.seq - Rodent sequence entries.
24. gbmam.seq - Other mammalian sequence entries.
25. gbvrt.seq - Other vertebrate sequence entries.
26. gbinv1.seq - Invertebrate sequence entries, part 1.
27. gbinv2.seq - Invertebrate sequence entries, part 2.
28. gbinv3.seq - Invertebrate sequence entries, part 3.
29. gbpln1.seq - Plant sequence entries (including fungi and algae), part 1.
30. gbpln2.seq - Plant sequence entries (including fungi and algae), part 2.
31. gbpln3.seq - Plant sequence entries (including fungi and algae), part 3.
32. gbbct1.seq - Bacterial sequence entries, part 1.
33. gbbct2.seq - Bacterial sequence entries, part 2.
34. gbbct3.seq - Bacterial sequence entries, part 3.
35. gbvrl1.seq - Viral sequence entries, part 1.
36. gbvrl2.seq - Viral sequence entries, part 2.
37. gbphg.seq - Phage sequence entries.
38. gbsyn.seq - Synthetic and chimeric sequence entries.
39. gbuna.seq - Unannotated sequence entries.
40. gbest1.seq - EST (expressed sequence tag) sequence entries, part 1.
41. gbest2.seq - EST (expressed sequence tag) sequence entries, part 2.
42. gbest3.seq - EST (expressed sequence tag) sequence entries, part 3.
43. gbest4.seq - EST (expressed sequence tag) sequence entries, part 4.
44. gbest5.seq - EST (expressed sequence tag) sequence entries, part 5.
45. gbest6.seq - EST (expressed sequence tag) sequence entries, part 6.
46. gbest7.seq - EST (expressed sequence tag) sequence entries, part 7.
47. gbest8.seq - EST (expressed sequence tag) sequence entries, part 8.
48. gbest9.seq - EST (expressed sequence tag) sequence entries, part 9.
49. gbest10.seq - EST (expressed sequence tag) sequence entries, part 10.
50. gbest11.seq - EST (expressed sequence tag) sequence entries, part 11.
51. gbest12.seq - EST (expressed sequence tag) sequence entries, part 12.
52. gbest13.seq - EST (expressed sequence tag) sequence entries, part 13.
53. gbest14.seq - EST (expressed sequence tag) sequence entries, part 14.
54. gbest15.seq - EST (expressed sequence tag) sequence entries, part 15.
55. gbest16.seq - EST (expressed sequence tag) sequence entries, part 16.
56. gbest17.seq - EST (expressed sequence tag) sequence entries, part 17.
57. gbest18.seq - EST (expressed sequence tag) sequence entries, part 18.
58. gbest19.seq - EST (expressed sequence tag) sequence entries, part 19.
59. gbest20.seq - EST (expressed sequence tag) sequence entries, part 20.
60. gbest21.seq - EST (expressed sequence tag) sequence entries, part 21.
61. gbest22.seq - EST (expressed sequence tag) sequence entries, part 22.
62. gbest23.seq - EST (expressed sequence tag) sequence entries, part 23.
63. gbest24.seq - EST (expressed sequence tag) sequence entries, part 24.
64. gbest25.seq - EST (expressed sequence tag) sequence entries, part 25.
65. gbest26.seq - EST (expressed sequence tag) sequence entries, part 26.
66. gbest27.seq - EST (expressed sequence tag) sequence entries, part 27.
67. gbest28.seq - EST (expressed sequence tag) sequence entries, part 28.
68. gbest29.seq - EST (expressed sequence tag) sequence entries, part 29.
69. gbest30.seq - EST (expressed sequence tag) sequence entries, part 30.
70. gbest31.seq - EST (expressed sequence tag) sequence entries, part 31.
71. gbest32.seq - EST (expressed sequence tag) sequence entries, part 32.
72. gbest33.seq - EST (expressed sequence tag) sequence entries, part 33.
73. gbest34.seq - EST (expressed sequence tag) sequence entries, part 34.
74. gbest35.seq - EST (expressed sequence tag) sequence entries, part 35.
75. gbest36.seq - EST (expressed sequence tag) sequence entries, part 36.
76. gbest37.seq - EST (expressed sequence tag) sequence entries, part 37.
77. gbest38.seq - EST (expressed sequence tag) sequence entries, part 38.
78. gbest39.seq - EST (expressed sequence tag) sequence entries, part 39.
79. gbest40.seq - EST (expressed sequence tag) sequence entries, part 40.
80. gbest41.seq - EST (expressed sequence tag) sequence entries, part 41.
81. gbest42.seq - EST (expressed sequence tag) sequence entries, part 42.
82. gbest43.seq - EST (expressed sequence tag) sequence entries, part 43.
83. gbest44.seq - EST (expressed sequence tag) sequence entries, part 44.
84. gbest45.seq - EST (expressed sequence tag) sequence entries, part 45.
85. gbest46.seq - EST (expressed sequence tag) sequence entries, part 46.
86. gbest47.seq - EST (expressed sequence tag) sequence entries, part 47.
87. gbest48.seq - EST (expressed sequence tag) sequence entries, part 48.
88. gbest49.seq - EST (expressed sequence tag) sequence entries, part 49.
89. gbest50.seq - EST (expressed sequence tag) sequence entries, part 50.
90. gbest51.seq - EST (expressed sequence tag) sequence entries, part 51.
91. gbest52.seq - EST (expressed sequence tag) sequence entries, part 52.
92. gbest53.seq - EST (expressed sequence tag) sequence entries, part 53.
93. gbest54.seq - EST (expressed sequence tag) sequence entries, part 54.
94. gbest55.seq - EST (expressed sequence tag) sequence entries, part 55.
95. gbest56.seq - EST (expressed sequence tag) sequence entries, part 56.
96. gbest57.seq - EST (expressed sequence tag) sequence entries, part 57.
97. gbest58.seq - EST (expressed sequence tag) sequence entries, part 58.
98. gbest59.seq - EST (expressed sequence tag) sequence entries, part 59.
99. gbest60.seq - EST (expressed sequence tag) sequence entries, part 60.
100. gbest61.seq - EST (expressed sequence tag) sequence entries, part 61.
101. gbest62.seq - EST (expressed sequence tag) sequence entries, part 62.
102. gbest63.seq - EST (expressed sequence tag) sequence entries, part 63.
103. gbest64.seq - EST (expressed sequence tag) sequence entries, part 64.
104. gbest65.seq - EST (expressed sequence tag) sequence entries, part 65.
105. gbest66.seq - EST (expressed sequence tag) sequence entries, part 66.
106. gbest67.seq - EST (expressed sequence tag) sequence entries, part 67.
107. gbest68.seq - EST (expressed sequence tag) sequence entries, part 68.
108. gbest69.seq - EST (expressed sequence tag) sequence entries, part 69.
109. gbest70.seq - EST (expressed sequence tag) sequence entries, part 70.
110. gbest71.seq - EST (expressed sequence tag) sequence entries, part 71.
111. gbest72.seq - EST (expressed sequence tag) sequence entries, part 72.
112. gbest73.seq - EST (expressed sequence tag) sequence entries, part 73.
113. gbest74.seq - EST (expressed sequence tag) sequence entries, part 74.
114. gbest75.seq - EST (expressed sequence tag) sequence entries, part 75.
115. gbest76.seq - EST (expressed sequence tag) sequence entries, part 76.
116. gbest77.seq - EST (expressed sequence tag) sequence entries, part 77.
117. gbest78.seq - EST (expressed sequence tag) sequence entries, part 78.
118. gbest79.seq - EST (expressed sequence tag) sequence entries, part 79.
119. gbest80.seq - EST (expressed sequence tag) sequence entries, part 80.
120. gbest81.seq - EST (expressed sequence tag) sequence entries, part 81.
121. gbest82.seq - EST (expressed sequence tag) sequence entries, part 82.
122. gbest83.seq - EST (expressed sequence tag) sequence entries, part 83.
123. gbest84.seq - EST (expressed sequence tag) sequence entries, part 84.
124. gbest85.seq - EST (expressed sequence tag) sequence entries, part 85.
125. gbest86.seq - EST (expressed sequence tag) sequence entries, part 86.
126. gbest87.seq - EST (expressed sequence tag) sequence entries, part 87.
127. gbpat1.seq - Patent sequence entries, part 1.
128. gbpat2.seq - Patent sequence entries, part 2.
129. gbsts1.seq - STS (sequence tagged site) sequence entries, part 1.
130. gbsts2.seq - STS (sequence tagged site) sequence entries, part 2.
131. gbgss1.seq - GSS (genome survey sequence) sequence entries, part 1.
132. gbgss2.seq - GSS (genome survey sequence) sequence entries, part 2.
133. gbgss3.seq - GSS (genome survey sequence) sequence entries, part 3.
134. gbgss4.seq - GSS (genome survey sequence) sequence entries, part 4.
135. gbgss5.seq - GSS (genome survey sequence) sequence entries, part 5.
136. gbgss6.seq - GSS (genome survey sequence) sequence entries, part 6.
137. gbgss7.seq - GSS (genome survey sequence) sequence entries, part 7.
138. gbgss8.seq - GSS (genome survey sequence) sequence entries, part 8.
139. gbgss9.seq - GSS (genome survey sequence) sequence entries, part 9.
140. gbgss10.seq - GSS (genome survey sequence) sequence entries, part 10.
141. gbgss11.seq - GSS (genome survey sequence) sequence entries, part 11.
142. gbgss12.seq - GSS (genome survey sequence) sequence entries, part 12.
143. gbgss13.seq - GSS (genome survey sequence) sequence entries, part 13.
144. gbgss14.seq - GSS (genome survey sequence) sequence entries, part 14.
145. gbgss15.seq - GSS (genome survey sequence) sequence entries, part 15.
146. gbgss16.seq - GSS (genome survey sequence) sequence entries, part 16.
147. gbgss17.seq - GSS (genome survey sequence) sequence entries, part 17.
148. gbgss18.seq - GSS (genome survey sequence) sequence entries, part 18.
149. gbgss19.seq - GSS (genome survey sequence) sequence entries, part 19.
150. gbgss20.seq - GSS (genome survey sequence) sequence entries, part 20.
151. gbgss21.seq - GSS (genome survey sequence) sequence entries, part 21.
152. gbgss22.seq - GSS (genome survey sequence) sequence entries, part 22.
153. gbgss23.seq - GSS (genome survey sequence) sequence entries, part 23.
154. gbgss24.seq - GSS (genome survey sequence) sequence entries, part 24.
155. gbgss25.seq - GSS (genome survey sequence) sequence entries, part 25.
156. gbgss26.seq - GSS (genome survey sequence) sequence entries, part 26.
157. gbgss27.seq - GSS (genome survey sequence) sequence entries, part 27.
158. gbgss28.seq - GSS (genome survey sequence) sequence entries, part 28.
159. gbhtg1.seq - HTGS (high throughput genomic sequencing) sequence entries, part 1.
160. gbhtg2.seq - HTGS (high throughput genomic sequencing) sequence entries, part 2.
161. gbhtg3.seq - HTGS (high throughput genomic sequencing) sequence entries, part 3.
162. gbhtg4.seq - HTGS (high throughput genomic sequencing) sequence entries, part 4.
163. gbhtg5.seq - HTGS (high throughput genomic sequencing) sequence entries, part 5.
164. gbhtg6.seq - HTGS (high throughput genomic sequencing) sequence entries, part 6.
165. gbhtg7.seq - HTGS (high throughput genomic sequencing) sequence entries, part 7.
166. gbhtg8.seq - HTGS (high throughput genomic sequencing) sequence entries, part 8.
167. gbhtg9.seq - HTGS (high throughput genomic sequencing) sequence entries, part 9.
168. gbhtg10.seq - HTGS (high throughput genomic sequencing) sequence entries, part 10.
169. gbhtg11.seq - HTGS (high throughput genomic sequencing) sequence entries, part 11.
170. gbhtg12.seq - HTGS (high throughput genomic sequencing) sequence entries, part 12.
171. gbhtg13.seq - HTGS (high throughput genomic sequencing) sequence entries, part 13.
172. gbhtg14.seq - HTGS (high throughput genomic sequencing) sequence entries, part 14.
173. gbhtg15.seq - HTGS (high throughput genomic sequencing) sequence entries, part 15.
174. gbhtg16.seq - HTGS (high throughput genomic sequencing) sequence entries, part 16.
175. gbhtg17.seq - HTGS (high throughput genomic sequencing) sequence entries, part 17.
176. gbhtg18.seq - HTGS (high throughput genomic sequencing) sequence entries, part 18.
177. gbhtg19.seq - HTGS (high throughput genomic sequencing) sequence entries, part 19.
178. gbhtg20.seq - HTGS (high throughput genomic sequencing) sequence entries, part 20.
179. gbhtg21.seq - HTGS (high throughput genomic sequencing) sequence entries, part 21.
180. gbhtg22.seq - HTGS (high throughput genomic sequencing) sequence entries, part 22.
181. gbhtg23.seq - HTGS (high throughput genomic sequencing) sequence entries, part 23.
182. gbhtg24.seq - HTGS (high throughput genomic sequencing) sequence entries, part 24.
Three supplemental files provide the accession numbers of GenBank entries
that are new, updated, or deleted since the previous release:
a. gbchg.txt - Entries updated since the previous release.
b. gbdel.txt - Entries deleted since the previous release.
c. gbnew.txt - Entries new since the previous release.
An experimental file called gbcon.seq provides an alternative representation
for complex sequences, such as "segmented sets" and complete-genomes that have
been split into pieces. The GenBank README describes the experimental CON
division of GenBank in more detail:
ftp://ncbi.nlm.nih.gov/genbank/README.genbank
2.2.5 File Sizes
Uncompressed, the Release 120.0 flatfiles require roughly 36464 MB
(sequence files only) or 40709 MB (including the 'index' files). The
following table contains the approximate sizes of the individual files
in this release. Since minor changes to some of the files may occur
after these release notes have been written, these sizes should not be
used to determine file integrity; they are provided as an aid to planning
only.
File Size File Name
302809838 gbacc.idx
500060031 gbaut1.idx
512040546 gbaut2.idx
507371058 gbaut3.idx
505902850 gbaut4.idx
506329820 gbaut5.idx
504061205 gbaut6.idx
407643506 gbaut7.idx
250001306 gbbct1.seq
250003054 gbbct2.seq
97946544 gbbct3.seq
229693685 gbest1.seq
230689104 gbest10.seq
230690347 gbest11.seq
230688828 gbest12.seq
230688475 gbest13.seq
230687486 gbest14.seq
230690596 gbest15.seq
230688048 gbest16.seq
230689120 gbest17.seq
230689147 gbest18.seq
230689606 gbest19.seq
226682748 gbest2.seq
230689453 gbest20.seq
230689818 gbest21.seq
230689986 gbest22.seq
230690262 gbest23.seq
230689166 gbest24.seq
230687911 gbest25.seq
230689955 gbest26.seq
230690323 gbest27.seq
230689120 gbest28.seq
224953370 gbest29.seq
227464614 gbest3.seq
187533163 gbest30.seq
186999426 gbest31.seq
212996616 gbest32.seq
212486899 gbest33.seq
212685247 gbest34.seq
213868398 gbest35.seq
230689290 gbest36.seq
230688055 gbest37.seq
230012811 gbest38.seq
230688123 gbest39.seq
230688477 gbest4.seq
230689085 gbest40.seq
230687783 gbest41.seq
230691528 gbest42.seq
230687911 gbest43.seq
230689431 gbest44.seq
230689453 gbest45.seq
230688555 gbest46.seq
230690653 gbest47.seq
230689480 gbest48.seq
230689517 gbest49.seq
161964754 gbest5.seq
230687789 gbest50.seq
230690048 gbest51.seq
205535161 gbest52.seq
205848588 gbest53.seq
204977430 gbest54.seq
204633577 gbest55.seq
204712291 gbest56.seq
204800955 gbest57.seq
204834146 gbest58.seq
204801183 gbest59.seq
174674092 gbest6.seq
205914946 gbest60.seq
203182801 gbest61.seq
201578626 gbest62.seq
202420142 gbest63.seq
203198104 gbest64.seq
212795060 gbest65.seq
230690506 gbest66.seq
230689856 gbest67.seq
230687625 gbest68.seq
230690322 gbest69.seq
230689129 gbest7.seq
230690149 gbest70.seq
230688589 gbest71.seq
230689512 gbest72.seq
230689071 gbest73.seq
230688390 gbest74.seq
230687917 gbest75.seq
230688891 gbest76.seq
230688160 gbest77.seq
230689621 gbest78.seq
230688142 gbest79.seq
230688526 gbest8.seq
230688418 gbest80.seq
230690177 gbest81.seq
230687445 gbest82.seq
230689805 gbest83.seq
230687659 gbest84.seq
217104967 gbest85.seq
230687472 gbest86.seq
217192366 gbest87.seq
230690351 gbest9.seq
12765244 gbgen.idx
209717876 gbgss1.seq
209716703 gbgss10.seq
209716632 gbgss11.seq
209718868 gbgss12.seq
209716874 gbgss13.seq
209717364 gbgss14.seq
209717778 gbgss15.seq
209717958 gbgss16.seq
209717544 gbgss17.seq
209716304 gbgss18.seq
209718899 gbgss19.seq
209717013 gbgss2.seq
209719634 gbgss20.seq
209718756 gbgss21.seq
209717299 gbgss22.seq
209716578 gbgss23.seq
78822871 gbgss24.seq
250001796 gbgss25.seq
250001069 gbgss26.seq
250001720 gbgss27.seq
243403787 gbgss28.seq
209716850 gbgss3.seq
209718993 gbgss4.seq
209718292 gbgss5.seq
209718246 gbgss6.seq
209716617 gbgss7.seq
209717106 gbgss8.seq
209716352 gbgss9.seq
250144208 gbhtg1.seq
250189634 gbhtg10.seq
250136355 gbhtg11.seq
250019672 gbhtg12.seq
250070787 gbhtg13.seq
250002657 gbhtg14.seq
250080558 gbhtg15.seq
250052745 gbhtg16.seq
250073724 gbhtg17.seq
250118879 gbhtg18.seq
250113302 gbhtg19.seq
250040766 gbhtg2.seq
250149161 gbhtg20.seq
250206642 gbhtg21.seq
250196962 gbhtg22.seq
250321904 gbhtg23.seq
153644030 gbhtg24.seq
250183759 gbhtg3.seq
250206087 gbhtg4.seq
250071170 gbhtg5.seq
250050719 gbhtg6.seq
250039616 gbhtg7.seq
250062268 gbhtg8.seq
250030218 gbhtg9.seq
250008067 gbinv1.seq
250129848 gbinv2.seq
145940993 gbinv3.seq
267158869 gbjou.idx
218184126 gbkey.idx
80307593 gbmam.seq
250000462 gbpat1.seq
15767525 gbpat2.seq
12021437 gbphg.seq
250003949 gbpln1.seq
250082953 gbpln2.seq
231770545 gbpln3.seq
250027256 gbpri1.seq
250217776 gbpri2.seq
250000601 gbpri3.seq
250117184 gbpri4.seq
250038912 gbpri5.seq
250065054 gbpri6.seq
250013623 gbpri7.seq
100586624 gbpri8.seq
100394 gbrel.txt
248832960 gbrod.seq
728221856 gbsdr.txt
1034738 gbsec.idx
250002824 gbsts1.seq
64690608 gbsts2.seq
30823019 gbsyn.seq
6275752 gbuna.seq
250001244 gbvrl1.seq
99797536 gbvrl2.seq
141373518 gbvrt.seq
2.2.6 Per-Division Statistics
The following table provides a per-division breakdown of the number of
sequence entries and the total number of bases of DNA/RNA in each sequence
data file:
Division Entries Bases
BCT1 37811 97590863
BCT2 39092 97005331
BCT3 15246 35460324
EST1 68608 26482832
EST2 74341 28517557
EST3 73498 29811990
EST4 74676 28424158
EST5 48011 15238321
EST6 54631 17300903
EST7 75036 29535540
EST8 76115 30680585
EST9 77959 30168916
EST10 77220 30025846
EST11 75484 28912701
EST12 77932 30899604
EST13 77024 29207724
EST14 78344 31678583
EST15 74264 31349288
EST16 75707 33196023
EST17 83242 34236793
EST18 80438 32154905
EST19 78692 32167382
EST20 74347 30192383
EST21 74117 34343678
EST22 76065 30525688
EST23 77351 32360851
EST24 76196 34105195
EST25 72273 30671435
EST26 76164 31636538
EST27 77122 33426886
EST28 87581 40168497
EST29 95702 37805128
EST30 68857 18058909
EST31 68840 17922520
EST32 62490 18363452
EST33 43655 10663875
EST34 43225 10984365
EST35 43070 10515918
EST36 81549 32889207
EST37 99998 45430748
EST38 92610 46150471
EST39 78713 34204369
EST40 66038 28913530
EST41 72974 32262724
EST42 71558 30000443
EST43 83452 33644926
EST44 74582 29594702
EST45 70272 28607154
EST46 67254 30640048
EST47 78652 37339350
EST48 76154 36754345
EST49 74428 28619007
EST50 79341 29186668
EST51 82224 38767085
EST52 40160 10332033
EST53 40103 10083665
EST54 40138 11686600
EST55 40481 11398176
EST56 40544 10768420
EST57 40439 11417739
EST58 40310 11720417
EST59 40360 11026039
EST60 40130 10952579
EST61 40897 11456481
EST62 41327 10887425
EST63 41128 11353548
EST64 40925 11599340
EST65 51196 16688945
EST66 77326 32549239
EST67 75627 29372151
EST68 78412 39083387
EST69 76307 47991144
EST70 78216 43180936
EST71 78905 38714742
EST72 77515 43987167
EST73 74521 39389884
EST74 75063 30826368
EST75 74940 48380121
EST76 71425 30806481
EST77 76099 46873160
EST78 67488 36257253
EST79 98346 41009644
EST80 110486 38867391
EST81 73340 25979414
EST82 73177 27316828
EST83 74280 26401262
EST84 73031 26500906
EST85 77304 25833084
EST86 72600 29375661
EST87 71206 26888392
GSS1 84145 35510885
GSS2 83050 36609898
GSS3 84232 38962706
GSS4 74750 38576445
GSS5 72764 38877160
GSS6 70003 34846275
GSS7 73241 36845529
GSS8 71494 36624499
GSS9 69202 34307831
GSS10 66576 37383701
GSS11 71348 38145879
GSS12 62993 33497679
GSS13 67810 35234904
GSS14 68806 34514220
GSS15 66437 29703298
GSS16 65588 32918106
GSS17 71326 42888278
GSS18 65937 30827049
GSS19 56441 26272732
GSS20 52439 27237895
GSS21 53138 25419648
GSS22 54151 22811318
GSS23 79140 38225424
GSS24 31360 13575431
GSS25 87358 65490986
GSS26 72782 61182851
GSS27 72490 61778297
GSS28 87901 62848964
HTG1 1295 190326505
HTG2 1265 189946095
HTG3 1292 190179019
HTG4 1673 189643063
HTG5 2641 187280012
HTG6 2226 189657301
HTG7 2103 190798664
HTG8 1234 189310198
HTG9 1255 188836369
HTG10 1286 188484316
HTG11 1219 189253710
HTG12 1241 189054459
HTG13 17249 162999027
HTG14 19645 159779634
HTG15 4352 183303312
HTG16 4548 184271510
HTG17 5394 184276922
HTG18 4846 182016369
HTG19 1345 190284515
HTG20 1183 192097360
HTG21 1218 191580184
HTG22 1285 191261251
HTG23 1193 191846328
HTG24 695 116010628
INV1 8345 161196488
INV2 35907 118792009
INV3 28948 60464126
MAM 26751 23546914
PAT1 209865 68060088
PAT2 16306 3962186
PHG 1658 4494057
PLN1 41551 118513203
PLN2 36443 117437003
PLN3 44753 93917743
PRI1 6956 167156358
PRI2 1388 176950303
PRI3 15264 160559699
PRI4 19560 149912856
PRI5 12922 168940320
PRI6 24135 142005996
PRI7 36312 127816739
PRI8 28652 33950152
ROD 59362 97487781
STS1 89490 41017415
STS2 27338 10209930
SYN 12166 11248297
UNA 4142 1876985
VRL1 78328 62186033
VRL2 28024 30142204
VRT 45506 41846005
2.2.7 Selected Per-Organism Statistics
The following table provides the number of entries and bases of DNA/RNA for
the twenty most sequenced organisms in Release 120.0 (chloroplast and mitochon-
drial sequences not included):
Entries Bases Species
3607850 6382405671 Homo sapiens
2193379 1121171154 Mus musculus
144907 474560810 Drosophila melanogaster
180872 240287289 Arabidopsis thaliana
106504 199979196 Caenorhabditis elegans
188992 165536545 Tetraodon nigroviridis
158616 120327916 Oryza sativa
144058 73394214 Rattus norvegicus
140724 63054687 Bos taurus
126956 56233371 Glycine max
77526 47580979 Strongylocentrotus purpuratus
84471 46295326 Medicago truncatula
90258 46225904 Lycopersicon esculentum
52162 45752167 Giardia intestinalis
81008 39061208 Danio rerio
77260 36652378 Zea mays
18357 32780994 Saccharomyces cerevisiae
48130 30893247 Trypanosoma brucei
67299 29290692 Rattus sp.
56258 26686546 Chlamydomonas reinhardtii
2.2.8 Growth of GenBank
The following table lists the number of bases and the number of sequence
records in each release of GenBank, beginning with Release 3 in 1982.
Over the period 1982 to the present, the number of bases in GenBank
has doubled approximately every 14 months.
Release Date Base Pairs Entries
3 Dec 1982 680338 606
14 Nov 1983 2274029 2427
20 May 1984 3002088 3665
24 Sep 1984 3323270 4135
25 Oct 1984 3368765 4175
26 Nov 1984 3689752 4393
32 May 1985 4211931 4954
36 Sep 1985 5204420 5700
40 Feb 1986 5925429 6642
42 May 1986 6765476 7416
44 Aug 1986 8442357 8823
46 Nov 1986 9615371 9978
48 Feb 1987 10961380 10913
50 May 1987 13048473 12534
52 Aug 1987 14855145 14020
53 Sep 1987 15514776 14584
54 Dec 1987 16752872 15465
55 Mar 1988 19156002 17047
56 Jun 1988 20795279 18226
57 Sep 1988 22019698 19044
57.1 Oct 1988 23800000 20579
58 Dec 1988 24690876 21248
59 Mar 1989 26382491 22479
60 Jun 1989 31808784 26317
61 Sep 1989 34762585 28791
62 Dec 1989 37183950 31229
63 Mar 1990 40127752 33377
64 Jun 1990 42495893 35100
65 Sep 1990 49179285 39533
66 Dec 1990 51306092 41057
67 Mar 1991 55169276 43903
68 Jun 1991 65868799 51418
69 Sep 1991 71947426 55627
70 Dec 1991 77337678 58952
71 Mar 1992 83894652 65100
72 Jun 1992 92160761 71280
73 Sep 1992 101008486 78608
74 Dec 1992 120242234 97084
75 Feb 1993 126212259 106684
76 Apr 1993 129968355 111911
77 Jun 1993 138904393 120134
78 Aug 1993 147215633 131328
79 Oct 1993 157152442 143492
80 Dec 1993 163802597 150744
81 Feb 1994 173261500 162946
82 Apr 1994 180589455 169896
83 Jun 1994 191393939 182753
84 Aug 1994 201815802 196703
85 Oct 1994 217102462 215273
86 Dec 1994 230485928 237775
87 Feb 1995 248499214 269478
88 Apr 1995 286094556 352414
89 Jun 1995 318624568 425211
90 Aug 1995 353713490 492483
91 Oct 1995 384939485 555694
92 Dec 1995 425860958 620765
93 Feb 1996 463758833 685693
94 Apr 1996 499127741 744295
95 Jun 1996 551750920 835487
96 Aug 1996 602072354 920588
97 Oct 1996 651972984 1021211
98 Dec 1996 730552938 1114581
99 Feb 1997 786898138 1192505
100 Apr 1997 842864309 1274747
101 Jun 1997 966993087 1491069
102 Aug 1997 1053474516 1610848
103 Oct 1997 1160300687 1765847
104 Dec 1997 1258290513 1891953
105 Feb 1998 1372368913 2042325
106 Apr 1998 1502542306 2209232
107 Jun 1998 1622041465 2355928
108 Aug 1998 1797137713 2532359
109 Oct 1998 2008761784 2837897
110 Dec 1998 2162067871 3043729
111 Apr 1999 2569578208 3525418
112 Jun 1999 2974791993 4028171
113 Aug 1999 3400237391 4610118
114 Oct 1999 3841163011 4864570
115 Dec 1999 4653932745 5354511
116 Feb 2000 5805414935 5691170
117 Apr 2000 7376080723 6215002
118 Jun 2000 8604221980 7077491
119 Aug 2000 9545724824 8214339
120 Oct 2000 10335692655 9102634
3. FILE FORMATS
The flat file examples included in this section, while not always from the
current release, are usually fairly recent. Any differences compared to the
actual records are the result of updates to the entries involved.
3.1 File Header Information
With the exception of the index files, each of the 182 files of a
GenBank release begins with the same header, except for the first line,
which contains the file name, and the sixth line, which contains the
title of the file. The first line of the file contains the file name
in character positions 1 to 9 and the full database name (Genetic Sequence
Data Bank) starting in column 22. The brief names of the files in this
release are listed in section 2.2.
The second line contains the date of the current release in the form
`day month year', beginning in position 27. The fourth line contains
the current GenBank release number. The release number appears in
positions 48 to 52 and consists of three numbers separated by a decimal
point. The number to the left of the decimal is the major release
number. The digit to the right of the decimal indicates the version of
the major release; it is zero for the first version. The sixth line
contains a title for the file. The eighth line lists the number of
entries (loci), number of bases (or base pairs), and number of reports
of sequences (equal to number of entries in this case). These numbers are
right-justified at fixed positions. The number of entries appears in
positions 1 to 8, the number of bases in positions 16 to 26, and the
number of reports in positions 40 to 47. The third, fifth, seventh, and
ninth lines are blank.
1 10 20 30 40 50 60 70 79
---------+---------+---------+---------+---------+---------+---------+---------
GBBCT1.SEQ Genetic Sequence Data Bank
15 October 2000
NCBI-GenBank Flat File Release 120.0
Bacterial Sequences (Part 1)
37811 loci, 97585608 bases, from 37811 reported sequences
---------+---------+---------+---------+---------+---------+---------+---------
1 10 20 30 40 50 60 70 79
Example 1. Sample File Header
3.2 Directory Files
3.2.1 Short Directory File
The short directory file contains brief descriptions of all of the
sequence entries contained in this release. These descriptions are in
fifteen groups, one group for each of the fifteen sequence entry
data files. The first record at the beginning of a group of entries
contains the name of the group in uppercase characters, beginning in
position 21. The organism groups are PRIMATE, RODENT, OTHER MAMMAL,
OTHER VERTEBRATE, INVERTEBRATE, PLANT, BACTERIAL, STRUCTURAL RNA, VIRAL,
PHAGE, SYNTHETIC, UNANNOTATED, EXPRESSED SEQUENCE TAG, PATENT, or
SEQUENCE TAGGED SITE. The second record is blank.
Each record in the short directory contains the sequence entry name
(LOCUS) in the first 12 positions, followed by a brief definition of
the sequence beginning in column 13. The definition is truncated (at
the end of a word) to leave room at the right margin for at least one
space, the sequence length, and the letters `bp'. The length of the
sequence is printed right-justified to column 77, followed by the
letters `bp' in columns 78 and 79. The next-to-last record for a group
has `ZZZZZZZZZZ' in its first ten positions (where the entry name
would normally appear). The last record is a blank line. An example of
the short directory file format, showing the descriptions of the last
entries in the Other Vertebrate sequence data file and the first
entries of the Invertebrate sequence data file, is reproduced below:
1 10 20 30 40 50 60 70 79
---------+---------+---------+---------+---------+---------+---------+---------
ZEFWNT1G3 B.rerio wnt-1 gene (exon 3) for wnt-1 protein. 266bp
ZEFWNT1G4 B.rerio wnt-1 gene (exon 4) for wnt-1 protein. 647bp
ZEFZF54 Zebrafish homeotic gene ZF-54. 246bp
ZEFZFEN Zebrafish engrailed-like homeobox sequence. 327bp
ZZZZZZZZZZ
INVERTEBRATE
AAHAV33A Acanthocheilonema viteae pepsin-inhibitor-like-protein 1048bp
ACAAC01 Acanthamoeba castelani gene encoding actin I. 1571bp
ACAACTPH Acanthamoeba castellanii actophorin mRNA, complete cds. 671bp
ACAMHCA A.castellanii non-muscle myosin heavy chain gene, partial 5894bp
---------+---------+---------+---------+---------+---------+---------+---------
1 10 20 30 40 50 60 70 79
Example 2. Short Directory File
3.3 Index Files
There are six files containing indices to the entries in this release:
Accession number index file (Accession and Version)
Secondary accession number index file
Keyword phrase index file
Author name index file
Journal citation index file
Gene name index file
The index keys (accession numbers, keywords, authors, journals, and
gene symbols.) of an index are sorted alphabetically. All index keys
appear in uppercase characters even though they appear in mixed case
in the sequence entries. Beneath each index key, the identifiers of the
sequence entries containing that index key are listed (LOCUS name,
division abbreviation, and primary accession number). The following
codes are used to designate the data file divisions:
1. PRI - primate sequences
2. ROD - rodent sequences
3. MAM - other mammalian sequences
4. VRT - other vertebrate sequences
5. INV - invertebrate sequences
6. PLN - plant, fungal, and algal sequences
7. BCT - bacterial sequences
8. VRL - viral sequences
9. PHG - bacteriophage sequences
10. SYN - synthetic sequences
11. UNA - unannotated sequences
12. EST - EST sequences (expressed sequence tags)
13. PAT - patent sequences
14. STS - STS sequences (sequence tagged sites)
15. GSS - GSS sequences (genome survey sequences)
16. HTG - HTGS sequences (high throughput genomic sequences)
A line-oriented, TAB-delimited format is utilized for the gbaut.idx,
gbgen.idx, gbjou.idx, gbkey.idx, and gbsec.idx indexes. Each index
term is presented on its own line, and is followed by a
LOCUS/Division/Accession triplet for every record containing the term:
Indexed-Term
LOCUS-name1 Div-code1 Accession1
LOCUS-name2 Div-code2 Accession2
LOCUS-name3 Div-code3 Accession3
....
Here is an example of the format, in which TAB characters are displayed
as ^I, and carriage-returns/newlines as $ :
(H+,K+)-ATPASE BETA-SUBUNIT$
^IRATHKATPB^IROD^IM55655$
^IMUSATP4B1^IROD^IM64685$
^IMUSATP4B2^IROD^IM64686$
^IMUSATP4B3^IROD^IM64687$
^IMUSATP4B4^IROD^IM64688$
^IDOGATPASEB^IMAM^IM76486$
When viewed by a file browser such as 'less' or 'more' :
(H+,K+)-ATPASE BETA-SUBUNIT
RATHKATPB ROD M55655
MUSATP4B1 ROD M64685
MUSATP4B2 ROD M64686
MUSATP4B3 ROD M64687
MUSATP4B4 ROD M64688
DOGATPASEB MAM M76486
Note that the index terms can be distinguished from LOCUS/DIV/ACCESSION
by the fact that they do not start with a TAB character. So one can
extract just the terms via simple text-processing:
perl -ne 'print unless /^\s+/' < gbkey.idx > terms.gbkey
The format of the primary accession number index file is slightly
different, with each indexed term (Accession.Version) present on
the same line as the LOCUS/Division/Accession triplet:
Accession1.Version1 Locus-name1 Div-code1 Accession1
Accession2.Version2 Locus-name2 Div-code2 Accession2
....
Here is an example of the format, in which TAB characters are displayed
as ^I, and carriage-returns/newlines as $ :
AC000102.1^IAC000102^IPRI^IAC000102$
AC000103.1^IAC000103^IPLN^IAC000103$
AC000104.1^IF19P19^IPLN^IAC000104$
AC000105.40^IAC000105^IPRI^IAC000105$
AC000106.1^IF7G19^IPLN^IAC000106$
AC000107.1^IAC000107^IPLN^IAC000107$
AC000108.1^IAC000108^IBCT^IAC000108$
AC000109.1^IHSAC000109^IPRI^IAC000109$
AC000110.1^IHSAC000110^IPRI^IAC000110$
When viewed by a file browser such as 'less' or 'more' :
AC000102.1 AC000102 PRI AC000102
AC000103.1 AC000103 PLN AC000103
AC000104.1 F19P19 PLN AC000104
AC000105.40 AC000105 PRI AC000105
AC000106.1 F7G19 PLN AC000106
AC000107.1 AC000107 PLN AC000107
AC000108.1 AC000108 BCT AC000108
AC000109.1 HSAC000109 PRI AC000109
AC000110.1 HSAC000110 PRI AC000110
3.3.1 Accession Number Index File - gbacc.idx
Accession numbers are unique six character or eight-character alphanumeric
identifiers of GenBank database entries. The six-character accession
number format consists of a single uppercase letter, followed by 5 digits.
The eight-character accession number format consists of two uppercase
letters, followed by 6 digits. Accessions provide an unchanging identifier
for the data with which they are associated, and we encourage you to cite
accession numbers whenever you refer to data from GenBank.
GenBank entries can have both 'primary' and 'secondary' accessions
associated with them (see Section 3.5.6). Only primary accessions are present
in the gbacc.idx index.
3.3.2 Keyword Phrase Index File - gbkey.idx
Keyword phrases consist of names for gene products and other
characteristics of sequence entries.
3.3.3 Author Name Index File - gbaut*.idx
The author name index files list all of the author names that appear
in the references within sequence records.
3.3.4 Journal Citation Index File - gbjou.idx
The journal citation index file lists all of the citations that appear
in the references within sequence records.. All citations are truncated
to 80 characters.
3.3.5 Gene Name Index - gbgen.idx
The /gene qualifiers of many GenBank entries contain values other than
official gene symbols, such as the product or the standard name of the gene.
Hence, NCBI has chosen to build an index (gbgen.idx) more like a keyword index
for this field, using both the GenBank /gene qualifier and the 'Gene.locus'
fields from the NCBI internal database as keys.
3.4 Sequence Entry Files
GenBank releases contain one or more sequence entry data files, one
for each "division" of GenBank.
3.4.1 File Organization
Each of these files has the same format and consists of two parts:
header information (described in section 3.1) and sequence entries for
that division (described in the following sections).
3.4.2 Entry Organization
In the second portion of a sequence entry file (containing the
sequence entries for that division), each record (line) consists of
two parts. The first part is found in positions 1 to 10 and may
contain:
1. A keyword, beginning in column 1 of the record (e.g., REFERENCE is
a keyword).
2. A subkeyword beginning in column 3, with columns 1 and 2 blank
(e.g., AUTHORS is a subkeyword of REFERENCE).
3. Blank characters, indicating that this record is a continuation of
the information under the keyword or subkeyword above it.
4. A code, beginning in column 6, indicating the nature of an entry
(feature key) in the FEATURES table; these codes are described in
Section 3.4.12.1 below.
5. A number, ending in column 9 of the record. This number occurs in
the portion of the entry describing the actual nucleotide sequence and
designates the numbering of sequence positions.
6. Two slashes (//) in positions 1 and 2, marking the end of an entry.
The second part of each sequence entry record contains the information
appropriate to its keyword, in positions 13 to 80 for keywords and
positions 11 to 80 for the sequence.
The following is a brief description of each entry field. Detailed
information about each field may be found in Sections 3.4.4 to 3.4.14.
LOCUS - A short mnemonic name for the entry, chosen to suggest the
sequence's definition. Mandatory keyword/exactly one record.
DEFINITION - A concise description of the sequence. Mandatory
keyword/one or more records.
ACCESSION - The primary accession number is a unique, unchanging
code assigned to each entry. (Please use this code when citing
information from GenBank.) Mandatory keyword/one or more records.
VERSION - A compound identifier consisting of the primary
accession number and a numeric version number associated with the
current version of the sequence data in the record. This is followed
by an integer key (a "GI") assigned to the sequence by NCBI.
Mandatory keyword/exactly one record.
NID - An alternative method of presenting the NCBI GI
identifier (described above). The NID is obsolete and was removed
from the GenBank flatfile format in December 1999.
KEYWORDS - Short phrases describing gene products and other
information about an entry. Mandatory keyword in all annotated
entries/one or more records.
SEGMENT - Information on the order in which this entry appears in a
series of discontinuous sequences from the same molecule. Optional
keyword (only in segmented entries)/exactly one record.
SOURCE - Common name of the organism or the name most frequently used
in the literature. Mandatory keyword in all annotated entries/one or
more records/includes one subkeyword.
ORGANISM - Formal scientific name of the organism (first line)
and taxonomic classification levels (second and subsequent lines).
Mandatory subkeyword in all annotated entries/two or more records.
REFERENCE - Citations for all articles containing data reported
in this entry. Includes four subkeywords and may repeat. Mandatory
keyword/one or more records.
AUTHORS - Lists the authors of the citation. Mandatory
subkeyword/one or more records.
TITLE - Full title of citation. Optional subkeyword (present
in all but unpublished citations)/one or more records.
JOURNAL - Lists the journal name, volume, year, and page
numbers of the citation. Mandatory subkeyword/one or more records.
MEDLINE - Provides the Medline unique identifier for a
citation. Optional subkeyword/one record.
PUBMED - Provides the PubMed unique identifier for a
citation. Optional subkeyword/one record.
REMARK - Specifies the relevance of a citation to an
entry. Optional subkeyword/one or more records.
COMMENT - Cross-references to other sequence entries, comparisons to
other collections, notes of changes in LOCUS names, and other remarks.
Optional keyword/one or more records/may include blank records.
FEATURES - Table containing information on portions of the
sequence that code for proteins and RNA molecules and information on
experimentally determined sites of biological significance. Optional
keyword/one or more records.
BASE COUNT - Summary of the number of occurrences of each base
code in the sequence. Mandatory keyword/exactly one record.
ORIGIN - Specification of how the first base of the reported sequence
is operationally located within the genome. Where possible, this
includes its location within a larger genetic map. Mandatory
keyword/exactly one record.
- The ORIGIN line is followed by sequence data (multiple records).
// - Entry termination symbol. Mandatory at the end of an
entry/exactly one record.
3.4.3 Sample Sequence Data File
An example of a complete sequence entry file follows. (This example
has only two entries.) Note that in this example, as throughout the
data bank, numbers in square brackets indicate items in the REFERENCE
list. For example, in ACARR58S, [1] refers to the paper by Mackay, et
al.
1 10 20 30 40 50 60 70 79
---------+---------+---------+---------+---------+---------+---------+---------
GBSMP.SEQ Genetic Sequence Data Bank
15 December 1992
GenBank Flat File Release 74.0
Structural RNA Sequences
2 loci, 236 bases, from 2 reported sequences
LOCUS AAURRA 118 bp ss-rRNA RNA 16-JUN-1986
DEFINITION A.auricula-judae (mushroom) 5S ribosomal RNA.
ACCESSION K03160
VERSION K03160.1 GI:173593
KEYWORDS 5S ribosomal RNA; ribosomal RNA.
SOURCE A.auricula-judae (mushroom) ribosomal RNA.
ORGANISM Auricularia auricula-judae
Eukaryota; Fungi; Eumycota; Basidiomycotina; Phragmobasidiomycetes;
Heterobasidiomycetidae; Auriculariales; Auriculariaceae.
REFERENCE 1 (bases 1 to 118)
AUTHORS Huysmans,E., Dams,E., Vandenberghe,A. and De Wachter,R.
TITLE The nucleotide sequences of the 5S rRNAs of four mushrooms and
their use in studying the phylogenetic position of basidiomycetes
among the eukaryotes
JOURNAL Nucleic Acids Res. 11, 2871-2880 (1983)
FEATURES Location/Qualifiers
rRNA 1..118
/note="5S ribosomal RNA"
BASE COUNT 27 a 34 c 34 g 23 t
ORIGIN 5' end of mature rRNA.
1 atccacggcc ataggactct gaaagcactg catcccgtcc gatctgcaaa gttaaccaga
61 gtaccgccca gttagtacca cggtggggga ccacgcggga atcctgggtg ctgtggtt
//
LOCUS ABCRRAA 118 bp ss-rRNA RNA 15-SEP-1990
DEFINITION Acetobacter sp. (strain MB 58) 5S ribosomal RNA, complete sequence.
ACCESSION M34766
VERSION M34766.1 GI:173603
KEYWORDS 5S ribosomal RNA.
SOURCE Acetobacter sp. (strain MB 58) rRNA.
ORGANISM Acetobacter sp.
Prokaryotae; Gracilicutes; Scotobacteria; Aerobic rods and cocci;
Azotobacteraceae.
REFERENCE 1 (bases 1 to 118)
AUTHORS Bulygina,E.S., Galchenko,V.F., Govorukhina,N.I., Netrusov,A.I.,
Nikitin,D.I., Trotsenko,Y.A. and Chumakov,K.M.
TITLE Taxonomic studies of methylotrophic bacteria by 5S ribosomal RNA
sequencing
JOURNAL J. Gen. Microbiol. 136, 441-446 (1990)
FEATURES Location/Qualifiers
rRNA 1..118
/note="5S ribosomal RNA"
BASE COUNT 27 a 40 c 32 g 17 t 2 others
ORIGIN
1 gatctggtgg ccatggcggg agcaaatcag ccgatcccat cccgaactcg gccgtcaaat
61 gccccagcgc ccatgatact ctgcctcaag gcacggaaaa gtcggtcgcc gccagayy
//
---------+---------+---------+---------+---------+---------+---------+---------
1 10 20 30 40 50 60 70 79
Example 9. Sample Sequence Data File
3.4.4 LOCUS Format
The pieces of information contained in the LOCUS record are always
found in fixed positions. The locus name (or entry name), which is
always ten characters or less, begins in position 13. The locus name
is designed to help group entries with similar sequences: the first
three characters usually designate the organism; the fourth and fifth
characters can be used to show other group designations, such as gene
product; for segmented entries the last character is one of a series
of sequential integers.
The number of bases or base pairs in the sequence ends in position 29.
The letters `bp' are in positions 31 to 32. Positions 34 to 36 give
the number of strands of the sequence. Positions 37 to 40 give the
topology of molecule sequenced. If the sequence is of a special type,
a notation (such as `circular') is included in positions 43 to 52.
GenBank sequence entries are divided among sixteen different
divisions. Each entry's division is specified by a three-letter code
in positions 53 to 55. See Section 3.3 for an explanation of division
codes.
Positions 63 to 73 of the record contain the date the entry was
entered or underwent any substantial revisions, such as the addition
of newly published data, in the form dd-MMM-yyyy.
The detailed format for the LOCUS record is as follows:
Positions Contents
1-12 LOCUS
13-22 Locus name
23-29 Length of sequence, right-justified
31-32 bp
34-36 Blank, ss- (single-stranded), ds- (double-stranded), or
ms- (mixed-stranded)
37-40 Blank, DNA, RNA, tRNA (transfer RNA), rRNA (ribosomal RNA),
mRNA (messenger RNA), or uRNA (small nuclear RNA)
43-52 Blank (implies linear) or circular
53-55 The division code (see Section 3.3)
63-73 Date, in the form dd-MMM-yyyy (e.g., 15-MAR-1991)
3.4.5 DEFINITION Format
The DEFINITION record gives a brief description of the sequence,
proceeding from general to specific. It starts with the common name of
the source organism, then gives the criteria by which this sequence is
distinguished from the remainder of the source genome, such as the
gene name and what it codes for, or the protein name and mRNA, or some
description of the sequence's function (if the sequence is
non-coding). If the sequence has a coding region, the description may
be followed by a completeness qualifier, such as cds (complete coding
sequence). There is no limit on the number of lines that may be part
of the DEFINITION. The last line must end with a period.
3.4.5.1 DEFINITION Format for NLM Entries
The DEFINITION line for entries derived from journal-scanning at the NLM is
an automatically generated descriptive summary that accompanies each DNA and
protein sequence. It contains information derived from fields in a database
that summarize the most important attributes of the sequence. The DEFINITION
lines are designed to supplement the accession number and the sequence itself
as a means of uniquely and completely specifying DNA and protein sequences. The
following are examples of NLM DEFINITION lines:
NADP-specific isocitrate dehydrogenase [swine, mRNA, 1 gene, 1585 nt]
94 kda fiber cell beaded-filament structural protein [rats, lens, mRNA
Partial, 1 gene, 1873 nt]
inhibin alpha {promoter and exons} [mice, Genomic, 1 gene, 1102 nt, segment
1 of 2]
cefEF, cefG=acetyl coenzyme A:deacetylcephalosporin C o-acetyltransferase
[Acremonium chrysogenum, Genomic, 2 genes, 2639 nt]
myogenic factor 3, qmf3=helix-loop-helix protein [Japanese quails,
embryo, Peptide Partial, 246 aa]
The first part of the definition line contains information describing
the genes and proteins represented by the molecular sequences. This can
be gene locus names, protein names and descriptions that replace or augment
actual names. Gene and gene product are linked by "=". Any special
identifying terms are presented within brackets, such as: {promoter},
{N-terminal}, {EC 2.13.2.4}, {alternatively spliced}, or {3' region}.
The second part of the definition line is delimited by square brackets, '[]',
and provides details about the molecule type and length. The biological
source, i.e., genus and species or common name as cited by the author.
Developmental stage, tissue type and strain are included if available.
The molecule types include: Genomic, mRNA, Peptide. and Other Genomic
Material. Genomic molecules are assumed to be partial sequence unless
"Complete" is specified, whereas mRNA and peptide molecules are assumed
to be complete unless "Partial" is noted.
3.4.6 ACCESSION Format
This field contains a series of six-character and/or eight-character
identifiers called 'accession numbers'. The six-character accession
number format consists of a single uppercase letter, followed by 5 digits.
The eight-character accession number format consists of two uppercase
letters, followed by 6 digits. The 'primary', or first, of the accession
numbers occupies positions 13 to 18 (6-character format) or positions
13 to 20 (8-character format). Subsequent 'secondary' accession numbers
(if present) are separated from the primary, and from each other, by a
single space. In some cases, multiple lines of secondary accession
numbers might be present, starting at position 13.
The primary accession number of a GenBank entry provides a stable identifier
for the biological object that the entry represents. Accessions do not change
when the underlying sequence data or associated features change.
Secondary accession numbers arise for a number of reasons. For example, a
single accession number may initially be assigned to a sequence described in
a publication. If it is later discovered that the sequence must be entered
into the database as multiple entries, each entry would receive a new primary
accession number, and the original accession number would appear as a secondary
accession number on each of the new entries.
3.4.7 VERSION Format
This line contains two types of identifiers for a GenBank database entry:
a compound accession number and an NCBI GI identifier.
LOCUS AF181452 1294 bp DNA PLN 12-OCT-1999
DEFINITION Hordeum vulgare dehydrin (Dhn2) gene, complete cds.
ACCESSION AF181452
VERSION AF181452.1 GI:6017929
^^^^^^^^^^ ^^^^^^^^^^
Compound NCBI GI
Accession Identifier
Number
A compound accession number consists of two parts: a stable, unchanging
primary-accession number portion (see Section 3.4.6 for a description of
accession numbers), and a sequentially increasing numeric version number.
The accession and version numbers are separated by a period. The initial
version number assigned to a new sequence is one. Compound accessions are
often referred to as "Accession.Version" .
An accession number allows one to retrieve the same biological object in the
database, regardless of any changes that are made to the entry over time. But
those changes can include changes to the sequence data itself, which is of
fundamental importance to many database users. So a numeric version number is
associated with the sequence data in every database entry. If an entry (for
example, AF181452) undergoes two sequence changes, its compound accession
number on the VERSION line would start as AF181452.1 . After the first sequence
change this would become: AF181452.2 . And after the second change: AF181452.3 .
The NCBI GI identifier of the VERSION line also serves as a method for
identifying the sequence data that has existed for a database entry over
time. GI identifiers are numeric values of one or more digits. Since they
are integer keys, they are less human-friendly than the Accession.Version
system described above. Returning to our example for AF181452, it was
initially assigned GI 6017929. If the sequence changes, a new integer GI will
be assigned, perhaps 7345003 . And after the second sequence change, perhaps
the GI would become 10456892 .
Why are both these methods for identifying the version of the sequence
associated with a database entry in use? For two reasons:
- Some data sources processed by NCBI for incorporation into its Entrez
sequence retrieval system do not version their own sequences.
- GIs provide a uniform, integer identifier system for every sequence
NCBI has processed. Some products and systems derived from (or reliant
upon) NCBI products and services prefer to use these integer identifiers
because they can all be processed the same way.
GenBank Releases contain only the most recent versions of all sequences
in the database. However, older versions can be obtained via GI-based or
Accession.Version-based queries with NCBI's web-Entrez and network-Entrez
application. A sequence revision history web page is also available:
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/girevhist
NOTE: All the version numbers for the compound Accession.Version identifier
system were initialized to a value of one in February 1999, when that
system was introduced.
3.4.8 KEYWORDS Format
The KEYWORDS field does not appear in unannotated entries, but is
required in all annotated entries. Keywords are separated by
semicolons; a "keyword" may be a single word or a phrase consisting of
several words. Each line in the keywords field ends in a semicolon;
the last line ends with a period. If no keywords are included in the
entry, the KEYWORDS record contains only a period.
3.4.9 SEGMENT Format
The SEGMENT keyword is used when two (or more) entries of known
relative orientation are separated by a short (<10 kb) stretch of DNA.
It is limited to one line of the form `n of m', where `n' is the
segment number of the current entry and `m' is the total number of
segments.
3.4.10 SOURCE Format
The SOURCE field consists of two parts. The first part is found after
the SOURCE keyword and contains free-format information including an
abbreviated form of the organism name followed by a molecule type;
multiple lines are allowed, but the last line must end with a period.
The second part consists of information found after the ORGANISM
subkeyword. The formal scientific name for the source organism (genus
and species, where appropriate) is found on the same line as ORGANISM.
The records following the ORGANISM line list the taxonomic
classification levels, separated by semicolons and ending with a
period.
3.4.11 REFERENCE Format
The REFERENCE field consists of five parts: the keyword REFERENCE, and
the subkeywords AUTHORS, TITLE (optional), JOURNAL, MEDLINE (optional),
PUBMED (optional), and REMARK (optional).
The REFERENCE line contains the number of the particular reference and
(in parentheses) the range of bases in the sequence entry reported in
this citation. Additional prose notes may also be found within the
parentheses. The numbering of the references does not reflect
publication dates or priorities.
The AUTHORS line lists the authors in the order in which they appear
in the cited article. Last names are separated from initials by a
comma (no space); there is no comma before the final `and'. The list
of authors ends with a period. The TITLE line is an optional field,
although it appears in the majority of entries. It does not appear in
unpublished sequence data entries that have been deposited directly
into the GenBank data bank, the EMBL Nucleotide Sequence Data Library,
or the DNA Data Bank of Japan. The TITLE field does not end with a
period.
The JOURNAL line gives the appropriate literature citation for the
sequence in the entry. The word `Unpublished' will appear after the
JOURNAL subkeyword if the data did not appear in the scientific
literature, but was directly deposited into the data bank. For
published sequences the JOURNAL line gives the Thesis, Journal, or
Book citation, including the year of publication, the specific
citation, or In press.
The MEDLINE line provides the National Library of Medicine's Medline
unique identifier for a citation (if known). Medline UIs are 8 digit
numbers.
The PUBMED line provides the PubMed unique identifier for a citation
(if known). PUBMED ids are numeric, and are record identifiers for article
abstracts in the PubMed database :
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
Citations in PubMed that do not fall within Medline's scope will have only
a PUBMED identifier. Similarly, citations that *are* in Medline's scope but
which have not yet been assigned Medline UIs will have only a PUBMED identifier.
If a citation is present in both the PubMed and Medline databases, both a
MEDLINE and a PUBMED line will be present.
The REMARK line is a textual comment that specifies the relevance
of the citation to the entry.
3.4.12 FEATURES Format
GenBank releases use a feature table format designed jointly by
GenBank, the EMBL Nucleotide Sequence Data Library, and the DNA Data
Bank of Japan. This format is in use by all three databases. The
most complete and accurate Feature Table documentation can be found
on the Web at:
http://www.ncbi.nlm.nih.gov/collab/FT/index.html
Any discrepancy between the abbreviated feature table description
of these release notes and the complete documentation on the Web
should be resolved in favor of the version at the above URL.
The Feature Table specification is also available as a printed
document: `The DDBJ/EMBL/GenBank Feature Table: Definition'. Contact
GenBank at the address shown on the first page of these Release Notes
if you would like a copy.
The feature table contains information about genes and gene products,
as well as regions of biological significance reported in the
sequence. The feature table contains information on regions of the
sequence that code for proteins and RNA molecules. It also enumerates
differences between different reports of the same sequence, and
provides cross-references to other data collections, as described in
more detail below.
The first line of the feature table is a header that includes the
keyword `FEATURES' and the column header `Location/Qualifier.' Each
feature consists of a descriptor line containing a feature key and a
location (see sections below for details). If the location does not
fit on this line, a continuation line may follow. If further
information about the feature is required, one or more lines
containing feature qualifiers may follow the descriptor line.
The feature key begins in column 6 and may be no more than 15
characters in length. The location begins in column 22. Feature
qualifiers begin on subsequent lines at column 22. Location,
qualifier, and continuation lines may extend from column 22 to 80.
Feature tables are required, due to the mandatory presence of the
source feature. The sections below provide a brief introduction to
the feature table format.
3.4.12.1 Feature Key Names
The first column of the feature descriptor line contains the feature
key. It starts at column 6 and can continue to column 20. The list of
valid feature keys is shown below.
Remember, the most definitive documentation for the feature table can
be found at:
http://www.ncbi.nlm.nih.gov/collab/FT/index.html
allele Obsolete; see variation feature key
attenuator Sequence related to transcription termination
C_region Span of the C immunological feature
CAAT_signal `CAAT box' in eukaryotic promoters
CDS Sequence coding for amino acids in protein (includes
stop codon)
conflict Independent sequence determinations differ
D-loop Displacement loop
D_segment Span of the D immunological feature
enhancer Cis-acting enhancer of promoter function
exon Region that codes for part of spliced mRNA
gene Region that defines a functional gene, possibly
including upstream (promotor, enhancer, etc)
and downstream control elements, and for which
a name has been assigned.
GC_signal `GC box' in eukaryotic promoters
iDNA Intervening DNA eliminated by recombination
intron Transcribed region excised by mRNA splicing
J_region Span of the J immunological feature
LTR Long terminal repeat
mat_peptide Mature peptide coding region (does not include stop codon)
misc_binding Miscellaneous binding site
misc_difference Miscellaneous difference feature
misc_feature Region of biological significance that cannot be described
by any other feature
misc_recomb Miscellaneous recombination feature
misc_RNA Miscellaneous transcript feature not defined by other RNA keys
misc_signal Miscellaneous signal
misc_structure Miscellaneous DNA or RNA structure
modified_base The indicated base is a modified nucleotide
mRNA Messenger RNA
mutation Obsolete: see variation feature key
N_region Span of the N immunological feature
old_sequence Presented sequence revises a previous version
polyA_signal Signal for cleavage & polyadenylation
polyA_site Site at which polyadenine is added to mRNA
precursor_RNA Any RNA species that is not yet the mature RNA product
prim_transcript Primary (unprocessed) transcript
primer Primer binding region used with PCR
primer_bind Non-covalent primer binding site
promoter A region involved in transcription initiation
protein_bind Non-covalent protein binding site on DNA or RNA
RBS Ribosome binding site
rep_origin Replication origin for duplex DNA
repeat_region Sequence containing repeated subsequences
repeat_unit One repeated unit of a repeat_region
rRNA Ribosomal RNA
S_region Span of the S immunological feature
satellite Satellite repeated sequence
scRNA Small cytoplasmic RNA
sig_peptide Signal peptide coding region
snRNA Small nuclear RNA
source Biological source of the sequence data represented by
a GenBank record. Mandatory feature, one or more per record.
For organisms that have been incorporated within the
NCBI taxonomy database, an associated /db_xref="taxon:NNNN"
qualifier will be present (where NNNNN is the numeric
identifier assigned to the organism within the NCBI taxonomy
database).
stem_loop Hair-pin loop structure in DNA or RNA
STS Sequence Tagged Site; operationally unique sequence that
identifies the combination of primer spans used in a PCR assay
TATA_signal `TATA box' in eukaryotic promoters
terminator Sequence causing transcription termination
transit_peptide Transit peptide coding region
transposon Transposable element (TN)
tRNA Transfer RNA
unsure Authors are unsure about the sequence in this region
V_region Span of the V immunological feature
variation A related population contains stable mutation
- (hyphen) Placeholder
-10_signal `Pribnow box' in prokaryotic promoters
-35_signal `-35 box' in prokaryotic promoters
3'clip 3'-most region of a precursor transcript removed in processing
3'UTR 3' untranslated region (trailer)
5'clip 5'-most region of a precursor transcript removed in processing
5'UTR 5' untranslated region (leader)
3.4.12.2 Feature Location
The second column of the feature descriptor line designates the
location of the feature in the sequence. The location descriptor
begins at position 22. Several conventions are used to indicate
sequence location.
Base numbers in location descriptors refer to numbering in the entry,
which is not necessarily the same as the numbering scheme used in the
published report. The first base in the presented sequence is numbered
base 1. Sequences are presented in the 5 to 3 direction.
Location descriptors can be one of the following:
1. A single base;
2. A contiguous span of bases;
3. A site between two bases;
4. A single base chosen from a range of bases;
5. A single base chosen from among two or more specified bases;
6. A joining of sequence spans;
7. A reference to an entry other than the one to which the feature
belongs (i.e., a remote entry), followed by a location descriptor
referring to the remote sequence;
8. A literal sequence (a string of bases enclosed in quotation marks).
A site between two residues, such as an endonuclease cleavage site, is
indicated by listing the two bases separated by a carat (e.g., 23^24).
A single residue chosen from a range of residues is indicated by the
number of the first and last bases in the range separated by a single
period (e.g., 23.79). The symbols < and > indicate that the end point
of the range is beyond the specified base number.
A contiguous span of bases is indicated by the number of the first and
last bases in the range separated by two periods (e.g., 23..79). The
symbols < and > indicate that the end point of the range is beyond the
specified base number. Starting and ending positions can be indicated
by base number or by one of the operators described below.
Operators are prefixes that specify what must be done to the indicated
sequence to locate the feature. The following are the operators
available, along with their most common format and a description.
complement (location): The feature is complementary to the location
indicated. Complementary strands are read 5 to 3.
join (location, location, .. location): The indicated elements should
be placed end to end to form one contiguous sequence.
order (location, location, .. location): The elements are found in the
specified order in the 5 to 3 direction, but nothing is implied about
the rationality of joining them.
group (location, location, .. location): The elements are related and
should be grouped together, but no order is implied.
one-of (location, location, .. location): The element can be any one,
but only one, of the items listed.
3.4.12.3 Feature Qualifiers
Qualifiers provide additional information about features. They take
the form of a slash (/) followed by a qualifier name and, if
applicable, an equal sign (=) and a qualifier value. Feature
qualifiers begin at column 22.
Qualifiers convey many types of information. Their values can,
therefore, take several forms:
1. Free text;
2. Controlled vocabulary or enumerated values;
3. Citations or reference numbers;
4. Sequences;
5. Feature labels.
Text qualifier values must be enclosed in double quotation marks. The
text can consist of any printable characters (ASCII values 32-126
decimal). If the text string includes double quotation marks, each set
must be `escaped' by placing a double quotation mark in front of it
(e.g., /note="This is an example of ""escaped"" quotation marks").
Some qualifiers require values selected from a limited set of choices.
For example, the `/direction' qualifier has only three values `left,'
`right,' or `both.' These are called controlled vocabulary qualifier
values. Controlled qualifier values are not case sensitive; they can
be entered in any combination of upper- and lowercase without changing
their meaning.
Citation or published reference numbers for the entry should be
enclosed in square brackets ([]) to distinguish them from other
numbers.
A literal sequence of bases (e.g., "atgcatt") should be enclosed in
quotation marks. Literal sequences are distinguished from free text by
context. Qualifiers that take free text as their values do not take
literal sequences, and vice versa.
The `/label=' qualifier takes a feature label as its qualifier.
Although feature labels are optional, they allow unambiguous
references to the feature. The feature label identifies a feature
within an entry; when combined with the accession number and the name
of the data bank from which it came, it is a unique tag for that
feature. Feature labels must be unique within an entry, but can be the
same as a feature label in another entry. Feature labels are not case
sensitive; they can be entered in any combination of upper-and
lowercase without changing their meaning.
The following is a partial list of feature qualifiers.
/anticodon Location of the anticodon of tRNA and the amino acid
for which it codes
/bound_moiety Moiety bound
/citation Reference to a citation providing the claim of or
evidence for a feature
/codon Specifies a codon that is different from any found in the
reference genetic code
/codon_start Indicates the first base of the first complete codon
in a CDS (as 1 or 2 or 3)
/cons_splice Identifies intron splice sites that do not conform to
the 5'-GT... AG-3' splice site consensus
/db_xref A database cross-reference; pointer to related information
in another database. A description of all cross-references
can be found at:
http://www.ncbi.nlm.nih.gov/collab/db_xref.html
/direction Direction of DNA replication
/EC_number Enzyme Commission number for the enzyme product of the
sequence
/evidence Value indicating the nature of supporting evidence
/frequency Frequency of the occurrence of a feature
/function Function attributed to a sequence
/gene Symbol of the gene corresponding to a sequence region (usable
with all features)
/label A label used to permanently identify a feature
/map Map position of the feature in free-format text
/mod_base Abbreviation for a modified nucleotide base
/note Any comment or additional information
/number A number indicating the order of genetic elements
(e.g., exons or introns) in the 5 to 3 direction
/organism Name of the organism that is the source of the
sequence data in the record.
/partial Differentiates between complete regions and partial ones
/phenotype Phenotype conferred by the feature
/product Name of a product encoded by a coding region (CDS)
feature
/pseudo Indicates that this feature is a non-functional
version of the element named by the feature key
/rpt_family Type of repeated sequence; Alu or Kpn, for example
/rpt_type Organization of repeated sequence
/rpt_unit Identity of repeat unit that constitutes a repeat_region
/standard_name Accepted standard name for this feature
/transl_except Translational exception: single codon, the translation
of which does not conform to the reference genetic code
/translation Amino acid translation of a coding region
/type Name of a strain if different from that in the SOURCE field
/usedin Indicates that feature is used in a compound feature
in another entry
3.4.12.4 Cross-Reference Information
One type of information in the feature table lists cross-references to
the annual compilation of transfer RNA sequences in Nucleic Acids
Research, which has kindly been sent to us on CD-ROM by Dr. Sprinzl.
Each tRNA entry of the feature table contains a /note= qualifier that
includes a reference such as `(NAR: 1234)' to identify code 1234 in
the NAR compilation. When such a cross-reference appears in an entry
that contains a gene coding for a transfer RNA molecule, it refers to
the code in the tRNA gene compilation. Similar cross-references in
entries containing mature transfer RNA sequences refer to the
companion compilation of tRNA sequences published by D.H. Gauss and M.
Sprinzl in Nucleic Acids Research.
3.4.12.5 Feature Table Examples
In the first example a number of key names, feature locations, and
qualifiers are illustrated, taken from different sequences. The first
table entry is a coding region consisting of a simple span of bases
and including a /gene qualifier. In the second table entry, an NAR
cross-reference is given (see the previous section for a discussion of
these cross-references). The third and fourth table entries use the
symbols `<`and `>' to indicate that the beginning or end of the
feature is beyond the range of the presented sequence. In the fifth
table entry, the symbol `^' indicates that the feature is between
bases.
1 10 20 30 40 50 60 70 79
---------+---------+---------+---------+---------+---------+---------+---------
CDS 5..1261
/product="alpha-1-antitrypsin precursor"
/map="14q32.1"
/gene="PI"
tRNA 1..87
/note="Leu-tRNA-CAA (NAR: 1057)"
/anticodon=(pos:35..37,aa:Leu)
mRNA 1..>66
/note="alpha-1-acid glycoprotein mRNA"
transposon <1..267
/note="insertion element IS5"
misc_recomb 105^106
/note="B.subtilis DNA end/IS5 DNA start"
conflict 258
/replace="t"
/citation=[2]
---------+---------+---------+---------+---------+---------+---------+---------
1 10 20 30 40 50 60 70 79
Example 10. Feature Table Entries
The next example shows the representation for a CDS that spans more
than one entry.
1 10 20 30 40 50 60 70 79
---------+---------+---------+---------+---------+---------+---------+---------
LOCUS HUMPGAMM1 3688 bp ds-DNA PRI 15-OCT-1990
DEFINITION Human phosphoglycerate mutase (muscle specific isozyme) (PGAM-M)
gene, 5' end.
ACCESSION M55673 M25818 M27095
KEYWORDS phosphoglycerate mutase.
SEGMENT 1 of 2
.
.
.
FEATURES Location/Qualifiers
CAAT_signal 1751..1755
/gene="PGAM-M"
TATA_signal 1791..1799
/gene="PGAM-M"
exon 1820..2274
/number=1
/EC_number="5.4.2.1"
/gene="PGAM-M"
intron 2275..2377
/number=1
/gene="PGAM2"
exon 2378..2558
/number=2
/gene="PGAM-M"
.
.
.
//
LOCUS HUMPGAMM2 677 bp ds-DNA PRI 15-OCT-1990
DEFINITION Human phosphoglycerate mutase (muscle specific isozyme) (PGAM-M),
exon 3.
ACCESSION M55674 M25818 M27096
KEYWORDS phosphoglycerate mutase.
SEGMENT 2 of 2
.
.
.
FEATURES Location/Qualifiers
exon 255..457
/number=3
/gene="PGAM-M"
intron order(M55673:2559..>3688,<1..254)
/number=2
/gene="PGAM-M"
mRNA join(M55673:1820..2274,M55673:2378..2558,255..457)
/gene="PGAM-M"
CDS join(M55673:1861..2274,M55673:2378..2558,255..421)
/note="muscle-specific isozyme"
/gene="PGAM2"
/product="phosphoglycerate mutase"
/codon_start=1
/translation="MATHRLVMVRHGESTWNQENRFCGWFDAELSEKGTEEAKRGAKA
IKDAKMEFDICYTSVLKRAIRTLWAILDGTDQMWLPVVRTWRLNERHYGGLTGLNKAE
TAAKHGEEQVKIWRRSFDIPPPPMDEKHPYYNSISKERRYAGLKPGELPTCESLKDTI
ARALPFWNEEIVPQIKAGKRVLIAAHGNSLRGIVKHLEGMSDQAIMELNLPTGIPIVY
ELNKELKPTKPMQFLGDEETVRKAMEAVAAQGKAK"
.
.
.
//
---------+---------+---------+---------+---------+---------+---------+---------
1 10 20 30 40 50 60 70 79
Example 11. Joining Sequences
3.4.13 ORIGIN Format
The ORIGIN record may be left blank, may appear as `Unreported.' or
may give a local pointer to the sequence start, usually involving an
experimentally determined restriction cleavage site or the genetic
locus (if available). The ORIGIN record ends in a period if it
contains data, but does not include the period if the record is left
empty (in contrast to the KEYWORDS field which contains a period
rather than being left blank).
3.4.14 SEQUENCE Format
The nucleotide sequence for an entry is found in the records following
the ORIGIN record. The sequence is reported in the 5 to 3 direction.
There are sixty bases per record, listed in groups of ten bases
followed by a blank, starting at position 11 of each record. The
number of the first nucleotide in the record is given in columns 4 to
9 (right justified) of the record.
4. ALTERNATE RELEASES
NCBI is supplying sequence data in the GenBank flat file format to
maintain compatibility with existing software which require that
particular format. Although we have made every effort to ensure
that these data are presented in the traditional flat file format,
if you encounter any problems in using these data with software which
is based upon the flat file format, please contact us at:
[email protected]
The flat file is just one of many possible report formats that can be
generated from the richer representation supported by the ASN.1 form of the
data. Developers of new software tools should consider using the ASN.1 form
directly to take advantage of those features. Documentation and a Software
Developer's Toolkit for ASN.1 are available through NCBI. You may call NCBI
at (301)496-2475, or subscribe to a developers' electronic newsgroup by
sending your name, address, affiliation, and e-mail address to:
[email protected]
The Software Developer's Toolkit and PostScript documentation for UNIX,
VMS, Ultrix, AIX, MacOS, DOS, and Microsoft Windows systems is available
in a compressed UNIX tar file by anonymous ftp from 'ncbi.nlm.nih.gov',
in the toolbox/ncbi_tools directory. The file is 'ncbi.tar.Z'.
5. KNOWN PROBLEMS OF THE GENBANK DATABASE
5.1 Incorrect Gene Symbols in Entries and Index
The /gene qualifier for many GenBank entries contains values other than the
official gene symbol, such as the product or the standard name of the gene. The
gene symbol index (gbgen.idx) is created from the data in the /gene qualifier
and therefore may contain data other than official gene symbols.
6. GENBANK ADMINISTRATION
The National Center for Biotechnology Information (NCBI), National Library
of Medicine, National Institutes of Health, is responsible for the production
and distribution of the NIH GenBank Sequence Database. NCBI distributes
GenBank sequence data by anonymous FTP, e-mail servers and other
network services. For more information, you may contact NCBI at the
e-mail address: [email protected] or by phone: 301-496-2475.
6.1 Registered Trademark Notice
GenBank (R) is a registered trademark of the U.S. Department of Health
and Human Services for the Genetic Sequence Data Bank.
6.2 Citing GenBank
If you have used GenBank in your research, we would appreciate it if
you would include a reference to GenBank in all publications related
to that research.
When citing data in GenBank, it is appropriate to give the sequence
name, primary accession number, and the publication in which the
sequence first appeared. If the data are unpublished, we urge you to
contact the group which submitted the data to GenBank to see if there
is a recent publication or if they have determined any revisions or
extensions of the data.
It is also appropriate to list a reference for GenBank itself. The
following publication, which describes the GenBank database, should
be cited:
Benson D.A., Karsch-Mizrachi I., Lipman D.J., Ostell J., Rapp B.A.,
Wheeler D.L. GenBank. Nucl. Acids Res. 28(1):15-18 (2000)
The following statement is an example of how you may cite GenBank
data. It cites the sequence, its primary accession number, the group
who determined the sequence, and GenBank. The numbers in parentheses
refer to the GenBank citation above and to the REFERENCE in the
GenBank sequence entry.
`We scanned the GenBank (1) database for sequence similarities and
found one sequence (2), GenBank accession number J01016, which showed
significant similarity...'
(1) Benson, D.A. et al. Nucl. Acids Res. 28(1):15-18 (2000)
(2) Nellen, W. and Gallwitz, D. J. Mol. Biol. 159, 1-18 (1982)
6.3 GenBank Distribution Formats and Media
Complete flat file releases of the GenBank database are available only via
anonymous ftp from :
ftp://ncbi.nlm.nih.gov
Each release is cumulative, incorporating all previous GenBank data.
No retrieval software is provided. GenBank distribution via CD-ROM
ceased as of GenBank Release 106.0 (April, 1998).
6.4 Other Methods of Accessing GenBank Data
Entrez is a molecular biology database system that presents an integrated
view of DNA and protein sequence data, 3D structure data, complete genomes,
and associated MEDLINE entries. The system is produced by the National
Center for Biotechnology Information (NCBI), and is available only via
the Internet (using the Web-Entrez and Network-Entrez applications).
Accessing Entrez is easy: if you have a World Wide Web browser, such as
Netscape or Internet-Explorer, simply point your browser to:
http://www.ncbi.nlm.nih.gov/
The Web version of Entrez has all the capabilities of the network version,
but with the visual style of the World Wide Web. If you prefer the "look and
feel" of Network-Entrez, you may download Network-Entrez from the NCBI's
FTP server:
ftp://ncbi.nlm.nih.gov/
Versions are available for PC/Windows, Macintosh and several Unix variants.
For information about Network-Entrez, Web-Entrez or any other NCBI
services, you may contact NCBI by e-mail to [email protected] or by
phone at 301-496-2475.
6.5 Request for Corrections and Comments
We welcome your suggestions for improvements to GenBank. We are
especially interested to learn of errors or inconsistencies in the
data. BankIt or Sequin can be used to submit revisions to previous
submissions. In addition, suggestions and corrections can be sent by
electronic mail to: [email protected]. Please be certain to
indicate the GenBank release number (e.g., Release 120.0) and the
primary accession number of the entry to which your comments apply; it
is helpful if you also give the entry name and the current contents of
any data field for which you are recommending a change.
6.6 Credits and Acknowledgments
Credits -
GenBank Release Coordination
Mark Cavanaugh
GenBank Submission Coordination
Ilene Mizrachi
GenBank Annotation Staff
John Anderson, Maureen Beanan, Matthew Beyers, Medha Bhagwat,
Lori Black, Larry Chlumsky, Karen Clark, Irene Fang, Michael
Fetchko, Jeff Gilmour, Irene Kim, Pierre Ledoux, Richard
McVeigh, Leonie Misquitta, Michael Murphy, Cynthia
Rothblum-Oviatt, Quy Phung, Leigh Riley, Susan Schafer, Suh-suh
Wang, Jane Weisemann, Steven Wilhite, Sandhya Xirasagar,
Roxanne Yamashita and Linda Yankie
Data Management and Preparation
Vladimir Alekseyev, Serge Bazhin, Anton Butanaev, Mark Cavanaugh,
Hsiu-Chuan Chen, Jim Ostell, Joel Plotkin, Sergei Shavirin,
Karl Sirotkin, Vladimir Soussov, Tatiana Tatusov, Carolyn Tolstoshev,
Jane Weisemann
Database Administration
Slava Khotomliansky, Eugene Yaschenko
Production and User Support
Dennis Benson, Peter Cooper, Jim Fleshman, Susan Kimball,
Renata McCarthy, Scott McGinnis, Monica Romiti, Barbara Rapp,
Steven Rosenthal, Rose Marie Woodsmall, David Wheeler
Project Direction
David Lipman
Acknowledgments -
Contractor support for GenBank production and distribution
has been provided by Management Systems Designers, Inc.,
ComputerCraft Corporation, and The KEVRIC Company, Inc.
6.7 Disclaimer
The United States Government makes no representations or warranties
regarding the content or accuracy of the information. The United States
Government also makes no representations or warranties of merchantability
or fitness for a particular purpose or that the use of the sequences will
not infringe any patent, copyright, trademark, or other rights. The
United States Government accepts no responsibility for any consequence
of the receipt or use of the information.
For additional information about GenBank releases, please contact
NCBI by e-mail at [email protected], by phone at (301) 496-2475,
or by mail at:
GenBank
National Library of Medicine
Bldg. 38A Rm. 8N-809
8600 Rockville Pike
Bethesda, MD 20894
FAX: (301) 480-9241
