Defa4 defensin, alpha, 4 [Mus musculus (house mouse)] - Gene

Summary

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Official Symbol: Defa4provided by MGI
Official Full Name: defensin, alpha, 4provided by MGI
Primary source: MGI:MGI:99584
See related: AllianceGenome:MGI:99584
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Crp4; Defa28; Defcr4
Summary: Enables phospholipid binding activity. Involved in killing of cells of another organism. Acts upstream of or within antibacterial humoral response and disruption of plasma membrane integrity in another organism. Located in extracellular space. Is expressed in duodenum and ileum. Orthologous to several human genes including DEFA6 (defensin alpha 6). [provided by Alliance of Genome Resources, Nov 2024]
Annotation information: not in current annotation release
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Genomic context

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See Defa4 in Genome Data Viewer

Location:: 8; 8 A2

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE(-/-) Mouse Model.
Title: Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE(-/-) Mouse Model.
Depleting CRP4 is incompatible with a proper cardiac development and function. CRP4 is a novel antiremodeling factor regulated, at least to some extent, by cardiac cGMP.
Title: Amplified pathogenic actions of angiotensin II in cysteine-rich LIM-only protein 4-negative mouse hearts.
Backbone cyclization provides a means for engineeringalpha-defensins to improve their biological activity while providing excellent stability to serum degradation and highlighting the potential of peptide scaffolds for development of novel compounds.
Title: Biosynthesis and antimicrobial evaluation of backbone-cyclized α-defensins.
bactericidal activity predominantly affects noncommensal bacteria
Title: Bactericidal activity of mouse α-defensin cryptdin-4 predominantly affects noncommensal bacteria.
Lys-for-Arg replacements in cryptdin 4 attenuated bactericidal activity
Title: Electropositive charge in alpha-defensin bactericidal activity: functional effects of Lys-for-Arg substitutions vary with the peptide primary structure.
Beta-sheet-containing Crp4 induces membrane permeabilization more effectively than disulfide-null Crp4 by translocating and forming transient membrane defects.
Title: Mechanisms of alpha-defensin bactericidal action: comparative membrane disruption by Cryptdin-4 and its disulfide-null analogue.
CRP4 mediates cGMP/PKG stimulation of SM-specific gene expression, and PKG plays an important role in regulating the phenotype of VSMCs
Title: A cysteine-rich LIM-only protein mediates regulation of smooth muscle-specific gene expression by cGMP-dependent protein kinase.
Kinetics of Crp4 translocation coupled with peptide-induced vesicle leakage are reported.
Title: Kinetics of cryptdin-4 translocation coupled with peptide-induced vesicle leakage.
function of the conserved salt bridge in Crp4 is not linked to bactericidal activity or proteolytic stability of the mature peptide.
Title: Structural and functional characterization of the conserved salt bridge in mammalian paneth cell alpha-defensins: solution structures of mouse CRYPTDIN-4 and (E15D)-CRYPTDIN-4.
the prosegment of Crp4 inhibits Crp4 bactericidal activity by blocking peptide-mediated perturbation of target cell membranes
Title: Interactions of mouse Paneth cell alpha-defensins and alpha-defensin precursors with membranes. Prosegment inhibition of peptide association with biomimetic membranes.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1) 1 citation

General gene information

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Markers

Defa4 (e-PCR), detects polymorphism
- UniSTS:526855

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables phospholipid binding	IDA Inferred from Direct Assay more info	PubMed
enables pore-forming activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in antibacterial humoral response	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within antibacterial humoral response	IDA Inferred from Direct Assay more info	PubMed
involved_in antimicrobial humoral immune response mediated by antimicrobial peptide	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to lipopolysaccharide	IBA Inferred from Biological aspect of Ancestor more info
involved_in defense response to Gram-negative bacterium	IBA Inferred from Biological aspect of Ancestor more info
involved_in defense response to Gram-positive bacterium	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within defense response to bacterium	IDA Inferred from Direct Assay more info	PubMed
involved_in defense response to bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in disruption of plasma membrane integrity in another organism	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within disruption of plasma membrane integrity in another organism	IDA Inferred from Direct Assay more info	PubMed
involved_in innate immune response in mucosa	IBA Inferred from Biological aspect of Ancestor more info
involved_in killing of cells of another organism	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in azurophil granule	ISO Inferred from Sequence Orthology more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in transport vesicle	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: defensin alpha 4

Names: alpha-defensin 4; alpha-defensin, 28; defensin related cryptdin 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010039.2 → NP_034169.2 defensin alpha 4 precursor

See identical proteins and their annotated locations for NP_034169.2

Status: VALIDATED

Source sequence(s)

BC134360, BX634116

UniProtKB/Swiss-Prot

A3KPC1, P28311, Q9QZL4

Conserved Domains (2) summary

pfam00323
Location:64 → 89

Defensin_1; Mammalian defensin

pfam00879
Location:15 → 51

Defensin_propep; Defensin propeptide