Chek1 checkpoint kinase 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Chek1provided by RGD
Official Full Name: checkpoint kinase 1provided by RGD
Primary source: RGD:620545
See related: EnsemblRapid:ENSRNOG00000071217 AllianceGenome:RGD:620545
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables protein kinase activity. Involved in several processes, including cellular response to caffeine; negative regulation of DNA biosynthetic process; and negative regulation of G2/M transition of mitotic cell cycle. Predicted to be located in several cellular components, including centrosome; chromosome; and nucleoplasm. Predicted to be part of protein-containing complex. Predicted to be active in nucleus. Human ortholog(s) of this gene implicated in pancreatic cancer. Orthologous to human CHEK1 (checkpoint kinase 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Thymus (RPKM 60.4), Spleen (RPKM 33.5) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 8q22

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (44609417..44629867, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (36420565..36443477, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (39181162..39201588, complement)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Chaperone-mediated autophagy, in response to genotoxic stress, is regulated by degradation of activated Chk1.
Title: Regulated degradation of Chk1 by chaperone-mediated autophagy in response to DNA damage.
KIM-1 expression increased significantly in a manner that corresponded temporally and regionally with increased phosphorylation of checkpoint kinase 1 (Chk1) and STAT3 in acute kidney injury.
Title: A bioinformatics approach identifies signal transducer and activator of transcription-3 and checkpoint kinase 1 as upstream regulators of kidney injury molecule-1 after kidney injury.
Casein kinase 1 delta activity is modulated by Chk1-mediated phosphorylation.
Title: CK1δ kinase activity is modulated by Chk1-mediated phosphorylation.
Chk1 plays a role in regulating RALT protein stability by facilitating the interaction between 14-3-3 and RALT.
Title: Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3.
Chk1 is essential for neuronal survival, and perturbation of this pathway increases a cell's sensitivity to naturally accumulating DNA damage.
Title: Chk1 has an essential role in the survival of differentiated cortical neurons in the absence of DNA damage.
Akt prevents the translocation of BRCA1 to DNA damage foci and, thereby, inhibits the activation of Chk1 following DNA damage
Title: Akt activation emulates Chk1 inhibition and Bcl2 overexpression and abrogates G2 cell cycle checkpoint by inhibiting BRCA1 foci.
CHK1 is involved in affecting microtubule dynamics and could be inhibited for taxotere sensitization in CHK1 highly expressed tumor cells.
Title: CHK1 affects cell sensitivity to microtubule-targeted drugs.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH142972 (e-PCR)
- UniSTS:232219

RH136824 (e-PCR)
- UniSTS:218913

D8Got46 (e-PCR)
- UniSTS:113984

AI230581 (e-PCR)
- UniSTS:249949

D8Got212 (e-PCR)
- UniSTS:113609

AU028950 (e-PCR)
- UniSTS:111837

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables histone H3T11 kinase activity	ISO Inferred from Sequence Orthology more info
enables histone H3T11 kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA damage checkpoint signaling	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA damage checkpoint signaling	ISO Inferred from Sequence Orthology more info
involved_in DNA damage checkpoint signaling	ISO Inferred from Sequence Orthology more info
involved_in DNA damage checkpoint signaling	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within DNA damage response	ISO Inferred from Sequence Orthology more info
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
involved_in DNA damage response	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within DNA repair	ISO Inferred from Sequence Orthology more info
involved_in DNA repair	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within G2/M transition of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within apoptotic process involved in development	ISO Inferred from Sequence Orthology more info
involved_in cellular response to caffeine	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to mechanical stimulus	ISO Inferred from Sequence Orthology more info
involved_in chromatin remodeling	ISO Inferred from Sequence Orthology more info
involved_in chromatin remodeling	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within inner cell mass cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in mitotic G2 DNA damage checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic G2 DNA damage checkpoint signaling	ISO Inferred from Sequence Orthology more info
involved_in mitotic G2/M transition checkpoint	ISO Inferred from Sequence Orthology more info
involved_in mitotic G2/M transition checkpoint	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of DNA biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of G2/M transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of gene expression, epigenetic	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of gene expression, epigenetic	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of mitotic nuclear division	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of mitotic nuclear division	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within nucleus organization	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-threonine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-threonine phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of cell cycle	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in regulation of double-strand break repair via homologous recombination	ISO Inferred from Sequence Orthology more info
involved_in regulation of double-strand break repair via homologous recombination	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in regulation of mitotic centrosome separation	ISO Inferred from Sequence Orthology more info
involved_in regulation of mitotic centrosome separation	ISS Inferred from Sequence or Structural Similarity more info
involved_in signal transduction in response to DNA damage	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction in response to DNA damage	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in centrosome	ISS Inferred from Sequence or Structural Similarity more info
located_in chromatin	ISO Inferred from Sequence Orthology more info
located_in chromatin	ISS Inferred from Sequence or Structural Similarity more info
located_in chromosome, telomeric region	ISO Inferred from Sequence Orthology more info
located_in condensed nuclear chromosome	ISO Inferred from Sequence Orthology more info
located_in condensed nuclear chromosome	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
located_in replication fork	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: serine/threonine-protein kinase Chk1

Names: CHK1 checkpoint homolog; checkpoint kinase 1 homolog

NP_536325.1

EC 2.7.11.1

XP_008764264.1

EC 2.7.11.1

XP_008764265.1

EC 2.7.11.1

XP_038936653.1

EC 2.7.11.1

XP_038936654.1

EC 2.7.11.1

XP_063120868.1

EC 2.7.11.1

XP_063120869.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_080400.2 → NP_536325.1 serine/threonine-protein kinase Chk1

See identical proteins and their annotated locations for NP_536325.1

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000008

UniProtKB/Swiss-Prot

Q91ZN6, Q91ZN7

UniProtKB/TrEMBL

A0A8L2UQ45, A6KRN1

Related

ENSRNOP00000011226.4, ENSRNOT00000011226.7

Conserved Domains (1) summary

cd14069
Location:7 → 265

STKc_Chk1; Catalytic domain of the Serine/Threonine kinase, Checkpoint kinase 1

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086026.1 Reference GRCr8

Range

44609417..44629867 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_008766042.4 → XP_008764264.1 serine/threonine-protein kinase Chk1 isoform X1

See identical proteins and their annotated locations for XP_008764264.1

UniProtKB/Swiss-Prot

Q91ZN6, Q91ZN7

UniProtKB/TrEMBL

A0A8L2UQ45, A6KRN1

Conserved Domains (1) summary

cd14069
Location:7 → 265

STKc_Chk1; Catalytic domain of the Serine/Threonine kinase, Checkpoint kinase 1
XM_008766043.3 → XP_008764265.1 serine/threonine-protein kinase Chk1 isoform X1

See identical proteins and their annotated locations for XP_008764265.1

UniProtKB/Swiss-Prot

Q91ZN6, Q91ZN7

UniProtKB/TrEMBL

A0A8L2UQ45, A6KRN1

Conserved Domains (1) summary

cd14069
Location:7 → 265

STKc_Chk1; Catalytic domain of the Serine/Threonine kinase, Checkpoint kinase 1
XM_039080725.2 → XP_038936653.1 serine/threonine-protein kinase Chk1 isoform X2

Conserved Domains (1) summary

cl21453
Location:1 → 99

PKc_like; Protein Kinases, catalytic domain
XM_063264798.1 → XP_063120868.1 serine/threonine-protein kinase Chk1 isoform X2
XM_063264799.1 → XP_063120869.1 serine/threonine-protein kinase Chk1 isoform X4
XM_039080726.2 → XP_038936654.1 serine/threonine-protein kinase Chk1 isoform X3

Conserved Domains (1) summary

cd14069
Location:7 → 265

STKc_Chk1; Catalytic domain of the Serine/Threonine kinase, Checkpoint kinase 1