Dgka diacylglycerol kinase, alpha [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Dgkaprovided by RGD
Official Full Name: diacylglycerol kinase, alphaprovided by RGD
Primary source: RGD:70904
See related: EnsemblRapid:ENSRNOG00000022943 AllianceGenome:RGD:70904
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Dagk1
Summary: Predicted to enable ATP-dependent diacylglycerol kinase activity and phospholipid binding activity. Predicted to be involved in glycerolipid metabolic process; intracellular signal transduction; and lipid phosphorylation. Located in cytosol. Orthologous to human DGKA (diacylglycerol kinase alpha). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Thymus (RPKM 526.5), Spleen (RPKM 181.4) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 7q11

Exon count:: 23

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	7	NC_086025.1 (1733247..1761181, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	7	NC_051342.1 (1148734..1175324, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	7	NC_005106.4 (3143826..3170764, complement)

Chromosome 7 - NC_086025.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The expression of diacylglycerol kinase isoforms alpha and zeta correlates with the progression of experimental autoimmune encephalomyelitis in rats.
Title: The expression of diacylglycerol kinase isoforms α and ζ correlates with the progression of experimental autoimmune encephalomyelitis in rats.
The DGKalpha localize predominantly to the nucleus of ganglion cells.
Title: Distinct expression and localization of diacylglycerol kinase isozymes in rat retina.

Submit: New GeneRIF Correction

General gene information

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Markers

RH127677 (e-PCR)
- UniSTS:210987

d7mgh11 (e-PCR)
- UniSTS:122287

RH131702 (e-PCR)
- UniSTS:214985

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent diacylglycerol kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-dependent diacylglycerol kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent diacylglycerol kinase activity	ISO Inferred from Sequence Orthology more info
enables ATP-dependent diacylglycerol kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables alkylglycerol kinase activity	IEA Inferred from Electronic Annotation more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables kinase activity	ISO Inferred from Sequence Orthology more info
enables lipid binding	ISO Inferred from Sequence Orthology more info
enables phospholipid binding	IEA Inferred from Electronic Annotation more info
enables phospholipid binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in diacylglycerol metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in diacylglycerol metabolic process	IEA Inferred from Electronic Annotation more info
involved_in diacylglycerol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in diacylglycerol metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in glycerolipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in glycerolipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in lipid phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in lipid phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in phosphatidic acid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidic acid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidic acid biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidic acid biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: diacylglycerol kinase alpha

Names: 80 kDa diacylglycerol kinase; DAG kinase alpha; DGK-alpha; diglyceride kinase alpha

NP_542965.1

EC 2.7.1.107

EC 2.7.1.93

XP_008763234.1

EC 2.7.1.107

EC 2.7.1.93

XP_008763235.1

EC 2.7.1.107

EC 2.7.1.93

XP_008763236.1

EC 2.7.1.107

EC 2.7.1.93

XP_008763237.1

EC 2.7.1.107

EC 2.7.1.93

XP_008763239.1

EC 2.7.1.107

EC 2.7.1.93

XP_008763240.1

EC 2.7.1.107

EC 2.7.1.93

XP_008763241.1

EC 2.7.1.107

EC 2.7.1.93

XP_008763242.1

EC 2.7.1.107

EC 2.7.1.93

XP_017450122.1

EC 2.7.1.107

EC 2.7.1.93

XP_038934247.1

EC 2.7.1.107

EC 2.7.1.93

XP_038934248.1

EC 2.7.1.107

EC 2.7.1.93

XP_063119000.1

EC 2.7.1.107

EC 2.7.1.93

XP_063119002.1

EC 2.7.1.107

EC 2.7.1.93

XP_063119003.1

EC 2.7.1.107

EC 2.7.1.93

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_080787.1 → NP_542965.1 diacylglycerol kinase alpha

See identical proteins and their annotated locations for NP_542965.1

Status: PROVISIONAL

Source sequence(s)

S49760

UniProtKB/Swiss-Prot

P51556

UniProtKB/TrEMBL

A0A8I5ZK54

Related

ENSRNOP00000038628.7, ENSRNOT00000036373.7

Conserved Domains (7) summary

smart00045
Location:512 → 693

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:371 → 491

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG1597
Location:371 → 703

LCB5; Diacylglycerol kinase family enzyme [Lipid transport and metabolism, General function prediction only]

cd00051
Location:112 → 181

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:204 → 252

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam13499
Location:113 → 177

EF-hand_7; EF-hand domain pair

pfam14513
Location:6 → 107

DAG_kinase_N; Diacylglycerol kinase N-terminus

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086025.1 Reference GRCr8

Range

1733247..1761181 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_008765015.4 → XP_008763237.1 diacylglycerol kinase alpha isoform X4

See identical proteins and their annotated locations for XP_008763237.1

UniProtKB/TrEMBL

A0A8I5ZK54

Conserved Domains (7) summary

smart00045
Location:586 → 767

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:445 → 565

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG1597
Location:445 → 793

LCB5; Diacylglycerol kinase family enzyme [Lipid transport and metabolism, General function prediction only]

cd00051
Location:142 → 211

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:234 → 282

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam13499
Location:147 → 207

EF-hand_7; EF-hand domain pair

pfam14513
Location:6 → 107

DAG_kinase_N; Diacylglycerol kinase N-terminus
XM_008765020.4 → XP_008763242.1 diacylglycerol kinase alpha isoform X11

UniProtKB/TrEMBL

A0A8I5ZK54, A0A8L2QJB9

Conserved Domains (7) summary

smart00045
Location:542 → 723

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:401 → 521

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG1597
Location:401 → 733

LCB5; Diacylglycerol kinase family enzyme [Lipid transport and metabolism, General function prediction only]

cd00051
Location:142 → 211

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:234 → 282

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam13499
Location:147 → 207

EF-hand_7; EF-hand domain pair

pfam14513
Location:6 → 107

DAG_kinase_N; Diacylglycerol kinase N-terminus
XM_063262932.1 → XP_063119002.1 diacylglycerol kinase alpha isoform X9

UniProtKB/Swiss-Prot

P51556
XM_017594633.3 → XP_017450122.1 diacylglycerol kinase alpha isoform X4

UniProtKB/TrEMBL

A0A8I5ZK54

Conserved Domains (7) summary

smart00045
Location:586 → 767

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:445 → 565

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG1597
Location:445 → 793

LCB5; Diacylglycerol kinase family enzyme [Lipid transport and metabolism, General function prediction only]

cd00051
Location:142 → 211

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:234 → 282

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam13499
Location:147 → 207

EF-hand_7; EF-hand domain pair

pfam14513
Location:6 → 107

DAG_kinase_N; Diacylglycerol kinase N-terminus
XM_008765019.4 → XP_008763241.1 diacylglycerol kinase alpha isoform X8

UniProtKB/TrEMBL

A0A8I5ZK54, A0A8I6G415

Related

ENSRNOP00000080029.1, ENSRNOT00000102609.2

Conserved Domains (7) summary

smart00045
Location:548 → 729

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:407 → 527

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG1597
Location:407 → 739

LCB5; Diacylglycerol kinase family enzyme [Lipid transport and metabolism, General function prediction only]

cd00051
Location:148 → 217

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:240 → 288

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam13499
Location:149 → 213

EF-hand_7; EF-hand domain pair

pfam14513
Location:42 → 143

DAG_kinase_N; Diacylglycerol kinase N-terminus
XM_008765018.4 → XP_008763240.1 diacylglycerol kinase alpha isoform X7

UniProtKB/TrEMBL

A0A8I5ZK54

Conserved Domains (7) summary

smart00045
Location:548 → 729

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:407 → 527

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG1597
Location:407 → 755

LCB5; Diacylglycerol kinase family enzyme [Lipid transport and metabolism, General function prediction only]

cd00051
Location:148 → 217

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:240 → 288

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam13499
Location:149 → 213

EF-hand_7; EF-hand domain pair

pfam14513
Location:42 → 143

DAG_kinase_N; Diacylglycerol kinase N-terminus
XM_008765014.4 → XP_008763236.1 diacylglycerol kinase alpha isoform X3

Conserved Domains (7) summary

smart00045
Location:592 → 773

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:451 → 571

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG1597
Location:451 → 799

LCB5; Diacylglycerol kinase family enzyme [Lipid transport and metabolism, General function prediction only]

cd00051
Location:148 → 217

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:240 → 288

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam13499
Location:149 → 213

EF-hand_7; EF-hand domain pair

pfam14513
Location:42 → 143

DAG_kinase_N; Diacylglycerol kinase N-terminus
XM_063262930.1 → XP_063119000.1 diacylglycerol kinase alpha isoform X6
XM_008765017.4 → XP_008763239.1 diacylglycerol kinase alpha isoform X5

UniProtKB/TrEMBL

A0A8I5ZK54

Conserved Domains (7) summary

smart00045
Location:578 → 759

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:437 → 557

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG1597
Location:437 → 785

LCB5; Diacylglycerol kinase family enzyme [Lipid transport and metabolism, General function prediction only]

cd00051
Location:178 → 247

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:270 → 318

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam13499
Location:183 → 243

EF-hand_7; EF-hand domain pair

pfam14513
Location:42 → 143

DAG_kinase_N; Diacylglycerol kinase N-terminus
XM_008765013.4 → XP_008763235.1 diacylglycerol kinase alpha isoform X2

Conserved Domains (7) summary

smart00045
Location:622 → 803

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:481 → 601

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG1597
Location:481 → 813

LCB5; Diacylglycerol kinase family enzyme [Lipid transport and metabolism, General function prediction only]

cd00051
Location:178 → 247

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:270 → 318

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam13499
Location:183 → 243

EF-hand_7; EF-hand domain pair

pfam14513
Location:42 → 143

DAG_kinase_N; Diacylglycerol kinase N-terminus
XM_008765012.4 → XP_008763234.1 diacylglycerol kinase alpha isoform X1

Conserved Domains (7) summary

smart00045
Location:622 → 803

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:481 → 601

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG1597
Location:481 → 829

LCB5; Diacylglycerol kinase family enzyme [Lipid transport and metabolism, General function prediction only]

cd00051
Location:178 → 247

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:270 → 318

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam13499
Location:183 → 243

EF-hand_7; EF-hand domain pair

pfam14513
Location:42 → 143

DAG_kinase_N; Diacylglycerol kinase N-terminus
XM_039078320.2 → XP_038934248.1 diacylglycerol kinase alpha isoform X13

UniProtKB/TrEMBL

A6KSH7

Conserved Domains (3) summary

smart00045
Location:311 → 492

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:170 → 290

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd20890
Location:67 → 128

C1_DGKalpha_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase alpha (DAG kinase alpha) and similar proteins
XM_063262933.1 → XP_063119003.1 diacylglycerol kinase alpha isoform X10
XM_039078319.2 → XP_038934247.1 diacylglycerol kinase alpha isoform X12

Conserved Domains (5) summary

smart00046
Location:481 → 578

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00051
Location:178 → 247

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam14513
Location:42 → 143

DAG_kinase_N; Diacylglycerol kinase N-terminus

cd20890
Location:378 → 439

C1_DGKalpha_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase alpha (DAG kinase alpha) and similar proteins

cl00040
Location:265 → 325

C1; protein kinase C conserved region 1 (C1 domain) superfamily