Mcm3 minichromosome maintenance complex component 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mcm3provided by MGI
Official Full Name: minichromosome maintenance complex component 3provided by MGI
Primary source: MGI:MGI:101845
See related: Ensembl:ENSMUSG00000041859 AllianceGenome:MGI:101845
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: P1; Mcmd; p1.m; P1-MCM3
Summary: Predicted to enable single-stranded DNA binding activity. Predicted to contribute to single-stranded DNA helicase activity. Predicted to be involved in DNA metabolic process. Located in cytoplasm and nucleus. Is expressed in several structures, including brain; limb; myocardium layer; neural ectoderm; and retina. Orthologous to human MCM3 (minichromosome maintenance complex component 3). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in liver E14 (RPKM 48.6), liver E14.5 (RPKM 44.8) and 18 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 A4; 1 6.5 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (20873188..20890536, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (20802966..20820312, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Study characterize the interaction of Keap1, a central antioxidant response regulator in Metazoa, with the replicative helicase subunit protein MCM3. Analysis suggests that structural determinants of the interaction of Keap1 with its critical downstream target - Nrf2 master transactivator of oxidative stress response genes - may have evolved in evolution to mimic the conserved helix-2-insert motif of MCM3.
Title: Keap1-MCM3 interaction is a potential coordinator of molecular machineries of antioxidant response and genomic DNA replication in metazoa.
Mcm3-deficient erythroblasts display aberrant DNA replication patterns and fail to complete maturation, causing lethal anemia.
Title: Replication stress caused by low MCM expression limits fetal erythropoiesis and hematopoietic stem cell functionality.
These data reveal that CDK-dependent MCM3 phosphorylation contributes to the regulated formation of the MCM2-7 complex.
Title: Phosphorylation of MCM3 on Ser-112 regulates its incorporation into the MCM2-7 complex.
Depletion of Orc2 and Mcm3 by siRNA leads to an inhibition of cell proliferation, an altered cell cycle distribution as well as to multinucleated cells with insufficiently organised microtubules.
Title: Mouse pre-replicative complex proteins colocalise and interact with the centrosome.
interaction with GANP DNA-primase is associated with a novel phosphatase component G5PR
Title: MCM3-binding GANP DNA-primase is associated with a novel phosphatase component G5PR.
analysis of carboxyl-terminal MCM3 phosphorylation sites and their motifs
Title: Identification of carboxyl-terminal MCM3 phosphorylation sites using polyreactive phosphospecific antibodies.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH126856 (e-PCR)
- UniSTS:207136

RH124239 (e-PCR)
- UniSTS:161061

Mcm3 (e-PCR), detects polymorphism
- UniSTS:515973

X62154 (e-PCR)
- UniSTS:163244

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables forked DNA-dependent helicase activity	IEA Inferred from Electronic Annotation more info
enables four-way junction helicase activity	IEA Inferred from Electronic Annotation more info
enables single-stranded 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
contributes_to single-stranded DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in DNA replication initiation	IEA Inferred from Electronic Annotation more info
involved_in DNA strand elongation involved in DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA unwinding involved in DNA replication	ISO Inferred from Sequence Orthology more info
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via break-induced replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic DNA replication initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in premeiotic DNA replication	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
part_of CMG complex	ISO Inferred from Sequence Orthology more info
part_of CMG complex	ISS Inferred from Sequence or Structural Similarity more info
part_of MCM complex	IBA Inferred from Biological aspect of Ancestor more info
part_of MCM complex	ISO Inferred from Sequence Orthology more info
part_of MCM complex	ISS Inferred from Sequence or Structural Similarity more info
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	NAS Non-traceable Author Statement more info	PubMed
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: DNA replication licensing factor MCM3

Names: DNA polymerase alpha holoenzyme-associated protein P1; mini chromosome maintenance deficient; minichromosome maintenance deficient 3

NP_032589.1

EC 3.6.4.12

XP_030107786.1

EC 3.6.4.12

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008563.3 → NP_032589.1 DNA replication licensing factor MCM3

See identical proteins and their annotated locations for NP_032589.1

Status: VALIDATED

Source sequence(s)

AC156980, AC159614

Consensus CDS

CCDS14843.1

UniProtKB/Swiss-Prot

P25206, Q61492

UniProtKB/TrEMBL

Q3UI57, Q3ULD6, Q3UZH2

Related

ENSMUSP00000059192.10, ENSMUST00000053266.11

Conserved Domains (3) summary

smart00350
Location:109 → 654

MCM; minichromosome maintenance proteins

pfam14551
Location:17 → 129

MCM_N; MCM N-terminal domain

cl21455
Location:340 → 479

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

20873188..20890536 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030251926.2 → XP_030107786.1 DNA replication licensing factor MCM3 isoform X1

UniProtKB/TrEMBL

Q3UZH2

Conserved Domains (1) summary

smart00350
Location:63 → 608

MCM; minichromosome maintenance proteins