rrf-3 RNA-directed RNA polymerase [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: rrf-3
Official Full Name: RNA-directed RNA polymerase
Primary source: WormBase:WBGene00004510
Locus tag: CELE_F10B5.7
See related: AllianceGenome:WB:WBGene00004510
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable RNA-dependent RNA polymerase activity. Involved in regulatory ncRNA-mediated heterochromatin formation and spermatogenesis. Predicted to be part of nuclear RNA-directed RNA polymerase complex. Is expressed in excretory cell; head; intestine; nervous system; and tail. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See rrf-3 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 15

Sequence:: Chromosome: II; NC_003280.10 (8162465..8168651)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

C. elegans aversive olfactory learning generates diverse intergenerational effects.
Title: C. elegans aversive olfactory learning generates diverse intergenerational effects.
Results show that the tandem tudor protein ERI-5 potentiates ERI endo-RNAi by tethering an RNA-dependent RNA polymerase (RdRP, F10B5.7) module to DCR-1.
Title: Tudor domain ERI-5 tethers an RNA-dependent RNA polymerase to DCR-1 to potentiate endo-RNAi.
Present evidence for successive engagement of two different RdRPs (RRF-3/RRF-1) in an endogenous siRNA-based mechanism targeting specific mRNAs in C. elegans soma.
Title: Distinct phases of siRNA synthesis in an endogenous RNAi pathway in C. elegans soma.
the function of Caenorhabditis elegans RRF-3 during spermatogenesis
Title: A Caenorhabditis elegans RNA-directed RNA polymerase in sperm development and endogenous RNA interference.
These results provide additional support for the hypothesis that RRF-3 and ERI-1 act together in the endo-siRNA pathway, and furthermore suggests their involvement in additional biological processes.
Title: Whole genome microarray analysis of C. elegans rrf-3 and eri-1 mutants.
loss of this makes C. elegans hypersensitive to RNAi.
Title: Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

F10B5.7 (e-PCR)
- UniSTS:321348

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA-dependent RNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA-dependent RNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in RNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulatory ncRNA-mediated gene silencing	IEA Inferred from Electronic Annotation more info
involved_in regulatory ncRNA-mediated heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in siRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of nuclear RNA-directed RNA polymerase complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: RNA-directed RNA polymerase

NP_495713.2

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

8162465..8168651

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_063312.7 → NP_495713.2 RNA-directed RNA polymerase [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_495713.2

Status: REVIEWED

UniProtKB/TrEMBL

G5EE53

Conserved Domains (1) summary

pfam05183
Location:692 → 1221

RdRP; RNA dependent RNA polymerase