wago-4 Argonaute protein wago-4 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: wago-4
Official Full Name: Argonaute protein wago-4
Primary source: WormBase:WBGene00010263
Locus tag: CELE_F58G1.1
See related: AllianceGenome:WB:WBGene00010263
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable RNA endonuclease activity; miRNA binding activity; and single-stranded RNA binding activity. Involved in regulatory ncRNA-mediated heterochromatin formation. Acts upstream of or within with a positive effect on regulation of gene silencing by regulatory ncRNA. Predicted to be located in perinuclear region of cytoplasm. Predicted to be part of RISC complex. Predicted to be active in cytoplasmic ribonucleoprotein granule and nucleus. Is expressed in several structures, including EMS; Psub1; Psub2; germ line; and oocyte. [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See wago-4 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 11

Sequence:: Chromosome: II; NC_003280.10 (12919551..12923377)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

WAGO-4, necessary for the inheritance of RNA interference, was identified.
Title: A Cytoplasmic Argonaute Protein Promotes the Inheritance of RNAi.
MINA-1 and WAGO-4 are part of regulatory network coordinating germ cell death and RNAi in C. elegans.
Title: MINA-1 and WAGO-4 are part of regulatory network coordinating germ cell death and RNAi in C. elegans.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

F58G1.1 (e-PCR)
- UniSTS:306498

C06A1.4 (e-PCR)
- UniSTS:321188

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables miRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables single-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within_positive_effect regulation of gene silencing by regulatory ncRNA	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulatory ncRNA-mediated gene silencing	IEA Inferred from Electronic Annotation more info
involved_in regulatory ncRNA-mediated heterochromatin formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of RISC complex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasmic ribonucleoprotein granule	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Argonaute protein wago-4

NP_496751.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

12919551..12923377

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_064350.6 → NP_496751.1 Argonaute protein wago-4 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_496751.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O62275

Conserved Domains (3) summary

PLN03202
Location:67 → 897

PLN03202; protein argonaute; Provisional

cd02846
Location:316 → 426

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

pfam02171
Location:594 → 924

Piwi; Piwi domain