alg-4 Putative protein tag-76 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: alg-4
Official Full Name: Putative protein tag-76
Primary source: WormBase:WBGene00006449
Locus tag: CELE_ZK757.3
See related: AllianceGenome:WB:WBGene00006449
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable RNA endonuclease activity; miRNA binding activity; and single-stranded RNA binding activity. Involved in spermatogenesis. Predicted to be located in P granule. Predicted to be part of RISC complex. Predicted to be active in cytoplasmic ribonucleoprotein granule and nucleus. Is expressed in male. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See alg-4 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 13

Sequence:: Chromosome: III; NC_003281.10 (9887210..9891424, complement)

Chromosome III - NC_003281.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003281.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Small RNA-mediated genetic switches coordinate ALG-3/4 small RNA pathway function.
Title: Small RNA-mediated genetic switches coordinate ALG-3/4 small RNA pathway function.
Study shows that the CSR-1 Argonaute functions with ALG-3/4 to positively regulate target genes required for spermiogenesis; these findings are consistent with a model in which ALG-3/4 and CSR-1 and their associated small RNAs provide an epigenetic memory of paternal gene expression, thereby ensuring the transgenerational continuity of a robust spermiogenic program.
Title: Argonautes promote male fertility and provide a paternal memory of germline gene expression in C. elegans.
the role of two argonautes-class paralogs, alg-3 (T22B3.2) and alg-4 (ZK757.3), in promoting thermotolerant male fertility was described.
Title: Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans.

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General gene information

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Markers

ZK757.3 (e-PCR)
- UniSTS:306108

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables miRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables single-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IBA Inferred from Biological aspect of Ancestor more info
involved_in spermatogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in P granule	ISS Inferred from Sequence or Structural Similarity more info	PubMed
part_of RISC complex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info	PubMed
is_active_in cytoplasmic ribonucleoprotein granule	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Putative protein tag-76

NP_499191.1

Confirmed by transcript evidence

NP_499192.1

Confirmed by transcript evidence

NP_499193.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003281.10 Reference assembly

Range

9887210..9891424 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_066790.5 → NP_499191.1 Putative protein tag-76 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_499191.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P34681, Q7JMT0, Q9TW94

Conserved Domains (5) summary

PLN03202
Location:67 → 959

PLN03202; protein argonaute; Provisional

cd02846
Location:374 → 484

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:530 → 964

Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...

pfam08699
Location:266 → 306

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:89 → 235

ArgoN; N-terminal domain of argonaute
NM_066791.6 → NP_499192.1 Putative protein tag-76 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_499192.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P34681

Conserved Domains (5) summary

PLN03202
Location:64 → 956

PLN03202; protein argonaute; Provisional

cd02846
Location:371 → 481

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:527 → 961

Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...

pfam08699
Location:263 → 303

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:86 → 232

ArgoN; N-terminal domain of argonaute
NM_066792.4 → NP_499193.1 Putative protein tag-76 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_499193.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P34681