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sax-3 Protein sax-3 [ Caenorhabditis elegans ]

Gene ID: 180637, updated on 4-Jan-2025

Summary

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Official Symbol
sax-3
Official Full Name
Protein sax-3
Primary source
WormBase:WBGene00004729
Locus tag
CELE_ZK377.2
See related
AllianceGenome:WB:WBGene00004729
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Caenorhabditis elegans (strain: Bristol N2)
Lineage
Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary
Enables axon guidance receptor activity. Involved in generation of neurons and regulation of sensory neuron axon guidance. Located in plasma membrane. Is expressed in several structures, including QR.a; QR.p; muscle cell; neuroblasts; and neurons. Human ortholog(s) of this gene implicated in congenital nystagmus (multiple); hematologic cancer (multiple); panhypopituitarism; and vesicoureteral reflux. Orthologous to human ROBO1 (roundabout guidance receptor 1); ROBO2 (roundabout guidance receptor 2); and ROBO3 (roundabout guidance receptor 3). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See sax-3 in Genome Data Viewer
Location:
chromosome: X
Exon count:
12
Sequence:
Chromosome: X; NC_003284.9 (3451908..3465927, complement)

Chromosome X - NC_003284.9Genomic Context describing neighboring genes Neighboring gene FIP (Fungus-Induced Protein) Related Neighboring gene G-protein coupled receptors family 1 profile domain-containing protein Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene Uncharacterized protein Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene ncRNA

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_003284.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The conserved immunoglobulin superfamily member SAX-3/Robo directs multiple aspects of axon guidance in C. elegans. Zallen JA, et al. Cell, 1998 Jan 23. PMID 9458046
  2. Structural Principles in Robo Activation and Auto-inhibition. Barak R, et al. Cell, 2019 Apr 4. PMID 30853216
  3. Adhesive L1CAM-Robo Signaling Aligns Growth Cone F-Actin Dynamics to Promote Axon-Dendrite Fasciculation in C. elegans. Chen CH, et al. Dev Cell, 2019 Jan 28. PMID 30555000
  4. Adhesive L1CAM-Robo Signaling Aligns Growth Cone F-Actin Dynamics to Promote Axon-Dendrite Fasciculation in C. elegans. Chen CH, et al. Dev Cell, 2019 May 6. PMID 31063761
  5. Axon guidance. A Roundabout way of avoiding the midline. Dickson B. Nature, 1998 Jan 29. PMID 9461209

See all (89) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Conserved and divergent aspects of Robo receptor signaling and regulation between Drosophila Robo1 and C. elegans SAX-3.
    Title: Conserved and divergent aspects of Robo receptor signaling and regulation between Drosophila Robo1 and C. elegans SAX-3.
  2. Robo functions as an attractive cue for glial migration through SYG-1/Neph.
    Title: Robo functions as an attractive cue for glial migration through SYG-1/Neph.
  3. These data suggest that chw-1 is likely acting downstream and/or in parallel to sax-3 in axon guidance.
    Title: The Atypical Rho GTPase CHW-1 Works with SAX-3/Robo To Mediate Axon Guidance in Caenorhabditis elegans.
  4. SAX-3 binds to Wnt/CWN-2 and facilitates the membrane recruitment of CWN-2. SAX-3 forms a complex with the Ror/CAM-1 receptor and its downstream effector Dsh/DSH-1.
    Title: Robo and Ror function in a common receptor complex to regulate Wnt-mediated neurite outgrowth in Caenorhabditis elegans.
  5. SAX-3 regulate convergent extension-based nerve cord assembly in C. elegans
    Title: PCP and SAX-3/Robo Pathways Cooperate to Regulate Convergent Extension-Based Nerve Cord Assembly in C. elegans.
  6. We describe the effects that UNC-40 (DCC) and SAX-3 (Robo) receptors and the UNC-6, EGL-20, UNC-52, and SLT-1 extracellular cues have on directional bias of the axon outgrowth activity. the directional bias created by the cues depend on UNC-40 or SAX-3.
    Title: SAX-3 (Robo) and UNC-40 (DCC) regulate a directional bias for axon guidance in response to multiple extracellular cues.
  7. The SAX-3 receptor stimulates axon outgrowth and the signal sequence and transmembrane domain are critical for SAX-3 membrane localization in the posterior deirid neuron of C. elegans.
    Title: The SAX-3 receptor stimulates axon outgrowth and the signal sequence and transmembrane domain are critical for SAX-3 membrane localization in the PDE neuron of C. elegans.
  8. SAX-3 is required in the nervous system for proper extension of ventral anterior muscle processes.
    Title: Membrane extensions are associated with proper anterior migration of muscle cells during Caenorhabditis elegans embryogenesis.
  9. Axon outgrowth is not dependent on unc-40 or sax-3.
    Title: A developmental timing switch promotes axon outgrowth independent of known guidance receptors.
  10. EVA-1 identified as a transmembrane receptor for SLT-1; EVA-1 acts cell-autonomously for SLT-1/Slit-dependent axon migration functions of SAX-3/Robo, colocalizes with SAX-3 on cells & provides cell specificity to activation of SAX-3 signaling by SLT-1
    Title: The slit receptor EVA-1 coactivates a SAX-3/Robo mediated guidance signal in C. elegans.
Submit: New GeneRIF Correction See all GeneRIFs (12)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by WormBase

Function Evidence Code Pubs
enables axon guidance receptor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in Roundabout signaling pathway ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
involved_in axon guidance IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in cell-cell adhesion IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in dorsal/ventral axon guidance IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in neuron migration IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of sensory neuron axon guidance IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in sensory neuron axon guidance IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in system development IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in axon IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
Protein sax-3
NP_001024990.1
  • Confirmed by transcript evidence
NP_741748.2
  • Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003284.9 Reference assembly

    Range
    3451908..3465927 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001029819.7 → NP_001024990.1  Protein sax-3 [Caenorhabditis elegans]

    See identical proteins and their annotated locations for NP_001024990.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    G5EBF1, H2KYQ9
    Conserved Domains (5) summary
    cd05724
    Location:137 → 223
    Ig2_Robo; Second immunoglobulin (Ig)-like domain in Robo (roundabout) receptors
    smart00060
    Location:755 → 836
    FN3; Fibronectin type 3 domain
    cd00063
    Location:531 → 624
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    pfam07679
    Location:227 → 313
    I-set; Immunoglobulin I-set domain
    cl11960
    Location:30 → 129
    Ig; Immunoglobulin domain

  2. NM_171652.8 → NP_741748.2  Protein sax-3 [Caenorhabditis elegans]

    See identical proteins and their annotated locations for NP_741748.2

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    G5EBF1
    Conserved Domains (5) summary
    cd05724
    Location:137 → 223
    Ig2_Robo; Second immunoglobulin (Ig)-like domain in Robo (roundabout) receptors
    smart00060
    Location:755 → 836
    FN3; Fibronectin type 3 domain
    cd00063
    Location:531 → 624
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    pfam07679
    Location:227 → 313
    I-set; Immunoglobulin I-set domain
    cl11960
    Location:30 → 129
    Ig; Immunoglobulin domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
G5EBF1.1

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