Nkx2-2 NK2 homeobox 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nkx2-2provided by MGI
Official Full Name: NK2 homeobox 2provided by MGI
Primary source: MGI:MGI:97347
See related: Ensembl:ENSMUSG00000027434 AllianceGenome:MGI:97347
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Nkx2.2; tinman; Nkx-2.2
Summary: Enables DNA-binding transcription activator activity, RNA polymerase II-specific and cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within several processes, including enteroendocrine cell differentiation; neurogenesis; and positive regulation of cell differentiation. Located in nucleus. Is expressed in several structures, including central nervous system; genitourinary system; gut; neural ectoderm; and sensory organ. Orthologous to human NKX2-2 (NK2 homeobox 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in cerebellum adult (RPKM 1.9), CNS E11.5 (RPKM 1.6) and 10 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 G2; 2 72.63 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (146967909..147037270, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (147176485..147195434, complement)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The myelination-associated G protein-coupled receptor 37 is regulated by Zfp488, Nkx2.2, and Sox10 during oligodendrocyte differentiation.
Title: The myelination-associated G protein-coupled receptor 37 is regulated by Zfp488, Nkx2.2, and Sox10 during oligodendrocyte differentiation.
The transcription factor NKX2-2 regulates oligodendrocyte differentiation through domain-specific interactions with transcriptional corepressors.
Title: The transcription factor NKX2-2 regulates oligodendrocyte differentiation through domain-specific interactions with transcriptional corepressors.
The transient hypoxia at P7 altered the expression of Nkx2.2, resulting in delayed myelination in the external capsule.
Title: Region specific oligodendrocyte transcription factor expression in a model of neonatal hypoxic injury.
This suggests that Nkx2.2 is not only required in the early pancreatic progenitors, but has additional essential activities within the endocrine progenitor population.
Title: Genetic evidence that Nkx2.2 acts primarily downstream of Neurog3 in pancreatic endocrine lineage development.
Lmx1a is expressed in enterochromaffin cells and functions downstream of Nkx2.2.
Title: The novel enterochromaffin marker Lmx1a regulates serotonin biosynthesis in enteroendocrine cell lineages downstream of Nkx2.2.
nuclear import of Nkx2-2 is mediated not only by the classical import pathway but also directly by imp beta1 or imp 13
Title: Nuclear import of Nkx2-2 is mediated by multiple pathways.
Demonstrate that a rare mature enteroendocrine cell subpopulation that is demarcated by Nkx2.2 expression display stem cell properties during normal intestinal epithelial homeostasis, but is not easily activated upon injury.
Title: Nkx2.2 is expressed in a subset of enteroendocrine cells with expanded lineage potential.
that Nkx2.2 and Nkx2.9 proteins play no role in the development of branchiovisceral motor neurons in hindbrain rostral to rhombomere 4.
Title: Nkx2.2 and Nkx2.9 are the key regulators to determine cell fate of branchial and visceral motor neurons in caudal hindbrain.
These data identify Nkx2.2 as key regulator to determine neuronal subtypes in the p3 domain of the central nervous system.
Title: Generation of a Nkx2.2(Cre) knock-in mouse line: Analysis of cell lineages in the central nervous system.
The study demonstrates a previously unrecognized mechanism that controls insulin expression through c-Abl-regulated NKx2.2 and GLUT2.
Title: C-Abl inhibitor imatinib enhances insulin production by β cells: c-Abl negatively regulates insulin production via interfering with the expression of NKx2.2 and GLUT-2.

Submit: New GeneRIF Correction See all GeneRIFs (39)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (9) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

BB262845 (e-PCR)
- UniSTS:207420

UniSTS:238165 (e-PCR)
- UniSTS:238165

Nkx2-2 (e-PCR), detects polymorphism
- UniSTS:238165

PMC123023P4 (e-PCR)
- UniSTS:270425

Nkx2-2 (e-PCR), detects polymorphism
- UniSTS:144023

Nkx2-2 (e-PCR), detects polymorphism
- UniSTS:516529

NoName (e-PCR)
- UniSTS:489153

Nkx2-2 (e-PCR), detects polymorphism
- UniSTS:536534

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within astrocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within digestive tract development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within endocrine pancreas development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of neuron differentiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of neuron differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within nervous system development	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within neuroendocrine cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of neuron differentiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of neuron differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neuron fate specification	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within neuron fate specification	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within oligodendrocyte development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of oligodendrocyte differentiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in optic nerve development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within pancreatic A cell fate commitment	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within pancreatic A cell fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within pancreatic PP cell fate commitment	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of epithelial cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of gene expression	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of neuron differentiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of neuron differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of oligodendrocyte differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to glucose	IEA Inferred from Electronic Annotation more info
involved_in response to progesterone	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within smoothened signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within spinal cord motor neuron differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spinal cord oligodendrocyte cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within spinal cord oligodendrocyte cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spinal cord oligodendrocyte cell fate specification	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within type B pancreatic cell development	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within type B pancreatic cell development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of type B pancreatic cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within type B pancreatic cell fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within ventral spinal cord interneuron fate determination	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in intracellular anatomical structure	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: homeobox protein Nkx-2.2

Names: Drosophila NK2 transcription factor related, locus 2; NK2 transcription factor related, locus 2; glycoprotein GP330, renal; homeobox protein NK-2 homolog B; homeobox protein Nkx2-2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001077632.2 → NP_001071100.1 homeobox protein Nkx-2.2 isoform 2

See identical proteins and their annotated locations for NP_001071100.1

Status: VALIDATED

Source sequence(s)

AL928876, AL929317

Consensus CDS

CCDS38259.1

UniProtKB/TrEMBL

Q8BRS9

Related

ENSMUSP00000105596.4, ENSMUST00000109970.5

Conserved Domains (1) summary

pfam00046
Location:131 → 185

Homeobox; Homeobox domain
NM_001420453.1 → NP_001407382.1 homeobox protein Nkx-2.2 isoform 3

Status: VALIDATED

Source sequence(s)

AL928876, AL929317
NM_010919.3 → NP_035049.1 homeobox protein Nkx-2.2 isoform 1

See identical proteins and their annotated locations for NP_035049.1

Status: VALIDATED

Source sequence(s)

AL929317

Consensus CDS

CCDS16834.1

UniProtKB/Swiss-Prot

P42586

UniProtKB/TrEMBL

Q3URZ4

Related

ENSMUSP00000069666.6, ENSMUST00000067075.7

Conserved Domains (1) summary

pfam00046
Location:131 → 185

Homeobox; Homeobox domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

146967909..147037270 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036159329.1 → XP_036015222.1 homeobox protein Nkx-2.2 isoform X2

Conserved Domains (1) summary

pfam00046
Location:131 → 185

Homeobox; Homeobox domain
XM_036159345.1 → XP_036015238.1 homeobox protein Nkx-2.2 isoform X5

Conserved Domains (1) summary

pfam00046
Location:131 → 185

Homeobox; Homeobox domain
XM_036159330.1 → XP_036015223.1 homeobox protein Nkx-2.2 isoform X3

Conserved Domains (1) summary

pfam00046
Location:131 → 185

Homeobox; Homeobox domain
XM_036159341.1 → XP_036015234.1 homeobox protein Nkx-2.2 isoform X4

UniProtKB/TrEMBL

Q8BRS9

Conserved Domains (1) summary

pfam00046
Location:131 → 185

Homeobox; Homeobox domain
XM_036159331.1 → XP_036015224.1 homeobox protein Nkx-2.2 isoform X4

UniProtKB/TrEMBL

Q8BRS9

Conserved Domains (1) summary

pfam00046
Location:131 → 185

Homeobox; Homeobox domain
XM_036159335.1 → XP_036015228.1 homeobox protein Nkx-2.2 isoform X4

UniProtKB/TrEMBL

Q8BRS9

Conserved Domains (1) summary

pfam00046
Location:131 → 185

Homeobox; Homeobox domain
XM_036159347.1 → XP_036015240.1 homeobox protein Nkx-2.2 isoform X6

Conserved Domains (1) summary

pfam00046
Location:131 → 185

Homeobox; Homeobox domain
XM_006498900.5 → XP_006498963.1 homeobox protein Nkx-2.2 isoform X1

See identical proteins and their annotated locations for XP_006498963.1

UniProtKB/Swiss-Prot

P42586

UniProtKB/TrEMBL

Q3URZ4

Conserved Domains (1) summary

pfam00046
Location:131 → 185

Homeobox; Homeobox domain
XM_011239349.4 → XP_011237651.1 homeobox protein Nkx-2.2 isoform X1

See identical proteins and their annotated locations for XP_011237651.1

UniProtKB/Swiss-Prot

P42586

UniProtKB/TrEMBL

Q3URZ4

Conserved Domains (1) summary

pfam00046
Location:131 → 185

Homeobox; Homeobox domain
XM_006498899.4 → XP_006498962.1 homeobox protein Nkx-2.2 isoform X1

See identical proteins and their annotated locations for XP_006498962.1

UniProtKB/Swiss-Prot

P42586

UniProtKB/TrEMBL

Q3URZ4

Conserved Domains (1) summary

pfam00046
Location:131 → 185

Homeobox; Homeobox domain
XM_036159325.1 → XP_036015218.1 homeobox protein Nkx-2.2 isoform X1

UniProtKB/Swiss-Prot

P42586

UniProtKB/TrEMBL

Q3URZ4

Conserved Domains (1) summary

pfam00046
Location:131 → 185

Homeobox; Homeobox domain