rhgf-1 Guanine nucleotide exchange factor [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: rhgf-1
Official Full Name: Guanine nucleotide exchange factor
Primary source: WormBase:WBGene00006468
Locus tag: CELE_F13E6.6
See related: AllianceGenome:WB:WBGene00006468
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables microtubule binding activity. Involved in axon regeneration. Located in neuronal cell body and varicosity. Is expressed in nervous system and spermatheca. Human ortholog(s) of this gene implicated in immunodeficiency 62. Orthologous to several human genes including ARHGEF11 (Rho guanine nucleotide exchange factor 11). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See rhgf-1 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 26

Sequence:: Chromosome: X; NC_003284.9 (10697834..10711869, complement)

Chromosome X - NC_003284.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003284.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Tension-dependent RHGF-1 recruitment to stress fibers drives robust spermathecal tissue contraction.
Title: Tension-dependent RHGF-1 recruitment to stress fibers drives robust spermathecal tissue contraction.
Genetic interactions are consistent with RHO-1 and RHGF-1 acting with UNC-5, as well as with a parallel pathway, to regulate protrusion. The cytoskeletal interacting molecule UNC-33/CRMP was required for RHO-1 activity to inhibit MT accumulation, suggesting that UNC-33/CRMP might act downstream of RHO-1. In sum, these studies describe a new role of RHO-1 and RHGF-1 in regulation of growth cone protrusion by UNC-6/Netrin
Title: RHO-1 and the Rho GEF RHGF-1 interact with UNC-6/Netrin signaling to regulate growth cone protrusion and microtubule organization in Caenorhabditis elegans.
Independent of myosin light chain activation, RHGF-1 acts via Rho-dependent kinase LET-502/ROCK and activates a conserved, retrograde DLK-1 MAPK pathway, triggering synaptic branch retraction and overgrowth of the PLM neurite in a dose-dependent manner.
Title: RHGF-1/PDZ-RhoGEF and retrograde DLK-1 signaling drive neuronal remodeling on microtubule disassembly.
we show that TIAM-1 acts with UNC-40/DCC in axon guidance, suggesting that TIAM-1 might regulate growth cone protrusion via Rac GTPases in response to UNC-40/DCC
Title: The Rac GTP exchange factor TIAM-1 acts with CDC-42 and the guidance receptor UNC-40/DCC in neuronal protrusion and axon guidance.
The upstream GPA-12/RHGF-1 pathway stimulates only a subset of RHO-1 downstream effectors, suggesting that either the RHO-1 effectors require different levels of activated RHO-1 or there are two distinct pools of RHO-1 within C. elegans neurons.
Title: The Galpha12-RGS RhoGEF-RhoA signalling pathway regulates neurotransmitter release in C. elegans.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

F13E6.6 (e-PCR)
- UniSTS:309716

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables G protein-coupled receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables microtubule binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in axon regeneration	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in neuron projection	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in varicosity	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Guanine nucleotide exchange factor

NP_509791.4

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003284.9 Reference assembly

Range

10697834..10711869 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_077390.10 → NP_509791.4 Guanine nucleotide exchange factor [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_509791.4

Status: REVIEWED

UniProtKB/TrEMBL

G5EFE1, Q6T5A2

Conserved Domains (5) summary

smart00228
Location:49 → 125

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00029
Location:485 → 534

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00160
Location:743 → 940

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13329
Location:983 → 1090

PH_RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain

pfam09128
Location:246 → 443

RGS-like; Regulator of G protein signalling-like domain