Hspb6 heat shock protein family B (small) member 6 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Hspb6provided by RGD
Official Full Name: heat shock protein family B (small) member 6provided by RGD
Primary source: RGD:621554
See related: EnsemblRapid:ENSRNOG00000020922 AllianceGenome:RGD:621554
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: p20; Hsp20
Summary: Enables protein homodimerization activity and protein kinase binding activity. Involved in negative regulation of cardiac muscle cell apoptotic process. Predicted to be located in several cellular components, including cytosol; mitochondrion; and nuclear speck. Predicted to be active in cytoplasm and nucleus. Orthologous to human HSPB6 (heat shock protein family B (small) member 6). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Heart (RPKM 938.0), Muscle (RPKM 810.5) and 1 other tissue See more
Orthologs: human mouse all
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Genomic context

Location:: 1q21

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (94934372..94936516)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (85806898..85809072)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (89008117..89010256)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

activation of the cAMP-PKA pathway induces overexpression and partial phosphorylation of HspB6 lead to the inhibition of ASK1 expression.
Title: Overexpressed HspB6 Underlines a Novel Inhibitory Role in Kainic Acid-Induced Epileptic Seizure in Rats by Activating the cAMP-PKA Pathway.
we show for the first time that alphaB-crystallin, a molecular chaperon for tubulin/microtubules, is involved in cell shape determination. knockdown of this molecule caused myoblasts and glioma cells to lose their ability for adhesion as they tended to behave like migratory cells. knockdown in both C6 glial cells and L6 myoblast permitted cells to migrate more rapidly than dermal fibroblasts.
Title: Small Heat Shock Protein αB-Crystallin Controls Shape and Adhesion of Glioma and Myoblast Cells in the Absence of Stress.
Study delineates the protein kinase D1 binding sites on HSP20 and shows that HSP20 is a substrate for protein kinase D1.
Title: Heat shock protein 20 (HSP20) is a novel substrate for protein kinase D1 (PKD1).
formation of the Hsp20-PKD1 complex is essential for PKD1 nuclear translocation, signaling mechanisms leading to hypertrophy
Title: Small heat shock protein 20 (Hsp20) facilitates nuclear import of protein kinase D 1 (PKD1) during cardiac hypertrophy.
Hsp20-mesenchymal stem cells enhanced cardiac repair. The mechanisms contributing to the beneficial effects of Hsp20 were associated with enhanced Akt activation and increased secretion of growth factors (VEGF, FGF-2, and IGF-1).
Title: Hsp20-engineered mesenchymal stem cells are resistant to oxidative stress via enhanced activation of Akt and increased secretion of growth factors.
Here, crystal structures of excised alpha-crystallin domain from rat Hsp20 and that from human alphaB-crystallin show that they form homodimers with a shared groove at the interface by extending a beta sheet.
Title: Crystal structures of alpha-crystallin domain dimers of alphaB-crystallin and Hsp20.
Hsp20 expression prevents endotoxin-induced myocardial dysfunction and apoptosis via inhibition of NF-kappaB activation
Title: Overexpression of Hsp20 prevents endotoxin-induced myocardial dysfunction and apoptosis via inhibition of NF-kappaB activation.
Overexpression of Hsp20 inhibits doxorubicin-triggered cardiac injury, an effect that is dependent on AKT activation.
Title: Heat shock protein 20 interacting with phosphorylated Akt reduces doxorubicin-triggered oxidative stress and cardiotoxicity.
After 7 days of spinal cord transection, Hsp20 and Hsp25 levels in the soleus, plantaris, and adductor longus muscles were lower than in control rats, whereas Hsp20 was unchanged and Hsp25 increased in the tibialis anterior.
Title: Inactivity-induced modulation of Hsp20 and Hsp25 content in rat hindlimb muscles.
Secreased HSP20 is mediated by cAMP-dependent pathway, and impaired vasoconstriction in portal hypertension may be partially explained by decreased HSP20. Phosphorylation of HSP20 by PKA may alter HSP20 turnover.
Title: Effects of chronic portal hypertension on small heat-shock proteins in mesenteric arteries.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables protein folding chaperone	IEA Inferred from Electronic Annotation more info
enables protein folding chaperone	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-folding chaperone binding	IEA Inferred from Electronic Annotation more info
enables protein-folding chaperone binding	ISO Inferred from Sequence Orthology more info
enables structural constituent of eye lens	IEA Inferred from Electronic Annotation more info
enables unfolded protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables unfolded protein binding	IEA Inferred from Electronic Annotation more info
enables unfolded protein binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in chaperone-mediated protein folding	IEA Inferred from Electronic Annotation more info
involved_in chaperone-mediated protein folding	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of cardiac muscle cell apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in protein refolding	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to heat	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in extracellular region	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: heat shock protein beta-6

Names: heat shock 20 kDa-like protein p20; heat shock 20-kDa protein; heat shock protein family B (small) member B6; heat shock protein, alpha-crystallin-related, B6

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_138887.1 → NP_620242.1 heat shock protein beta-6

See identical proteins and their annotated locations for NP_620242.1

Status: PROVISIONAL

Source sequence(s)

D29960

UniProtKB/Swiss-Prot

P97541

UniProtKB/TrEMBL

A6J9Y5

Related

ENSRNOP00000028401.1, ENSRNOT00000028401.6

Conserved Domains (2) summary

cd06478
Location:66 → 148

ACD_HspB4-5-6; Alpha-crystallin domain found in alphaA-crystallin (HspB4), alphaB-crystallin (HspB5), and the small heat shock protein (sHsp) HspB6, also known as Hsp20. sHsps are molecular chaperones that suppress protein aggregation and protect against cell stress, ...

pfam00525
Location:3 → 58

Crystallin; Alpha crystallin A chain, N terminal

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

94934372..94936516

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063279355.1 → XP_063135425.1 heat shock protein beta-6 isoform X1

UniProtKB/TrEMBL

A0A8I6A0A0
XM_039092870.2 → XP_038948798.1 heat shock protein beta-6 isoform X2

Conserved Domains (2) summary

pfam00525
Location:3 → 58

Crystallin; Alpha crystallin A chain, N terminal

cl00175
Location:66 → 107

alpha-crystallin-Hsps_p23-like; alpha-crystallin domain (ACD) found in alpha-crystallin-type small heat shock proteins, and a similar domain found in p23 (a cochaperone for Hsp90) and in other p23-like proteins.