Spam1 sperm adhesion molecule 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Spam1provided by MGI
Official Full Name: sperm adhesion molecule 1provided by MGI
Primary source: MGI:MGI:109335
See related: Ensembl:ENSMUSG00000029682 AllianceGenome:MGI:109335
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ph-20
Summary: Enables hyalurononglucosaminidase activity. Acts upstream of or within single fertilization. Located in acrosomal vesicle; external side of plasma membrane; and membrane raft. Orthologous to human SPAM1 (sperm adhesion molecule 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Restricted expression toward testis adult (RPKM 9.7) See more
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Genomic context

Location:: 6 A3.1; 6 11.28 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (24791187..24801047)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (24791188..24801048)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A comparative analysis between WT and Spam1/Hyal5 dKO epididymal sperm revealed that the absence of these 2 sperm Hyals resulted in a marked accumulation of sperm on the outside of the cumulus-oocyte complex.
Title: SPAM1/HYAL5 double deficiency in male mice leads to severe male subfertility caused by a cumulus-oocyte complex penetration defect.
PH20 is elevated in demyelinating lesions and that increased PH20 expression is sufficient to inhibit oligodendrocyte precursor cell maturation and remyelination.
Title: Digestion products of the PH20 hyaluronidase inhibit remyelination.
ACR or PRSS21 or both may function cooperatively with SPAM1 in sperm/cumulus penetration
Title: Functional characterization of double-knockout mouse sperm lacking SPAM1 and ACR or SPAM1 and PRSS21 in fertilization.
The data from this paper suggests that the hyaluronan-degrading activity of the sperm hyaluronidase SPAM1 may not be required for fertilization, at least in the mouse.
Title: Hyaluronan-degrading activity of mouse sperm hyaluronidase is not required for fertilization?
SPAM1 may function in sperm entry into the cumulus and sperm penetration through the cumulus matrix.
Title: Functional roles of mouse sperm hyaluronidases, HYAL5 and SPAM1, in fertilization.
First documentation of vesicular interaction with the sperm plasma membrane provides a model for the possible transfer mechanism of SPAM1 and other glycosyl phosphatidylinositol (GPI)-linked proteins to the sperm surface in reproductive fluids.
Title: Investigating the role of murine epididymosomes and uterosomes in GPI-linked protein transfer to sperm using SPAM1 as a model.
sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg
Title: Mouse sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg.
Sperm adhesion molecule 1 (SPAM1) is distributed in the nonciliated epithelial cells. Efferent ducts have the most intense staining. Spam1 was also present in the accessory organs, the prostate, and seminal vesicles and its fluid.
Title: Spam1 (PH-20) expression in the extratesticular duct and accessory organs of the mouse: a possible role in sperm fluid reabsorption.
Spam1 is a multifunctional protein and that it is less restricted in its expression than previously reported
Title: Mouse Spam1 (PH-20) is a multifunctional protein: evidence for its expression in the female reproductive tract.
SPAM1 is implicated in fluid reabsorption and urine concentration
Title: Expression of SPAM1 (PH-20) in the murine kidney is not accompanied by hyaluronidase activity: evidence for potential roles in fluid and water reabsorption.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

U33958 (e-PCR)
- UniSTS:125726

AV039194 (e-PCR)
- UniSTS:165452

U33958 (e-PCR)
- UniSTS:179347

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables hyalurononglucosaminidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables hyalurononglucosaminidase activity	IDA Inferred from Direct Assay more info	PubMed
enables hyalurononglucosaminidase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in carbohydrate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in fusion of sperm to egg plasma membrane involved in single fertilization	IEA Inferred from Electronic Annotation more info
involved_in hyaluronan catabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within single fertilization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in acrosomal vesicle	IBA Inferred from Biological aspect of Ancestor more info
located_in acrosomal vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in external side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane raft	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: hyaluronidase PH-20

Names: hyal-PH20; hyaluronoglucosaminidase PH-20; sperm surface protein PH-20

NP_001073344.1

EC 3.2.1.35

NP_033267.2

EC 3.2.1.35

XP_006505088.1

EC 3.2.1.35

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001079875.2 → NP_001073344.1 hyaluronidase PH-20 precursor

See identical proteins and their annotated locations for NP_001073344.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.

Source sequence(s)

AK005638, AY920282

Consensus CDS

CCDS19947.1

UniProtKB/Swiss-Prot

P48794, Q7TSD6, Q812F4, Q9DAQ1

UniProtKB/TrEMBL

Q5D1J0

Related

ENSMUSP00000143944.2, ENSMUST00000202331.4

Conserved Domains (1) summary

pfam01630
Location:42 → 369

Glyco_hydro_56; Hyaluronidase
NM_009241.3 → NP_033267.2 hyaluronidase PH-20 precursor

See identical proteins and their annotated locations for NP_033267.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.

Source sequence(s)

AK005638, AY920282

Consensus CDS

CCDS19947.1

UniProtKB/Swiss-Prot

P48794, Q7TSD6, Q812F4, Q9DAQ1

UniProtKB/TrEMBL

Q5D1J0

Related

ENSMUSP00000143970.2, ENSMUST00000202569.4

Conserved Domains (1) summary

pfam01630
Location:42 → 369

Glyco_hydro_56; Hyaluronidase