Crb3 crumbs family member 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Crb3provided by MGI
Official Full Name: crumbs family member 3provided by MGI
Primary source: MGI:MGI:2670904
See related: Ensembl:ENSMUSG00000044279 AllianceGenome:MGI:2670904
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 5730439B18
Summary: Predicted to enable SH3 domain binding activity. Involved in positive regulation of cell junction assembly. Located in apical part of cell. Part of subapical complex. Is expressed in several structures, including central nervous system; early conceptus; and retina. Orthologous to human CRB3 (crumbs cell polarity complex component 3). [provided by Alliance of Genome Resources, Jan 2025]
Expression: Broad expression in duodenum adult (RPKM 28.1), colon adult (RPKM 24.9) and 17 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 D; 17 29.65 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (57368583..57372920)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (57062201..57065918)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CRB3 navigates Rab11 trafficking vesicles to promote gammaTuRC assembly during ciliogenesis.
Title: CRB3 navigates Rab11 trafficking vesicles to promote γTuRC assembly during ciliogenesis.
YAP promotes myelin and non-myelin genes transcription while the polarity protein Crb3, localized at the tips of the myelin sheath, activates the Hippo pathway to temper YAP activity, therefore allowing for optimal myelin growth.
Title: Optimal myelin elongation relies on YAP activation by axonal growth and inhibition by Crb3/Hippo pathway.
Crb3 knockdown in mammalian cells affects the dynamics of IFT particle movement.
Title: Apico-basal Polarity Determinants Encoded by crumbs Genes Affect Ciliary Shaft Protein Composition, IFT Movement Dynamics, and Cilia Length.
Crumbs3 sustains epithelial tissue morphogenesis and is essential to maintain apical membrane identity in kidney epithelial cells.
Title: Mouse Crumbs3 sustains epithelial tissue morphogenesis in vivo.
Crb3, a Crumbs isoform that determines epithelial apical domain identity, is required for airway differentiation by controlling the localization of the transcriptional regulator Yap.
Title: Crumbs3-Mediated Polarity Directs Airway Epithelial Cell Fate through the Hippo Pathway Effector Yap.
The presence of Crb3 in many organs and the regularity of Crb3 distribution in the process of mouse embryonic development indicate that Crb3 protein plays an important role in establishing and maintaining the polarity of mouse embryos.
Title: The expression and localization of Crb3 in developmental stages of the mice embryos and in different organs of 1-week-old female mice.
Data indicate that Crb3 (Crumbs3) is crucial for epithelial morphogenesis and plays a role in linking the apical membrane to the underlying ezrin-containing cytoskeleton.
Title: Crumbs3 is essential for proper epithelial development and viability.
the presence of CRB3 protein in the mouse retina
Title: Immunocytochemical evidence of the localization of the Crumbs homologue 3 protein (CRB3) in the developing and mature mouse retina.
Data show the deletion of Pals1 leads to the disruption of the apical localization of Crb polarity complex proteins Crb1, Crb2 and Crb3 in retinal progenitors and the adult retina.
Title: Genetic ablation of Pals1 in retinal progenitor cells models the retinal pathology of Leber congenital amaurosis.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (7) 1 citation

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables SH3 domain binding	IEA Inferred from Electronic Annotation more info
enables SH3 domain binding	ISO Inferred from Sequence Orthology more info
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell-cell junction organization	ISA Inferred from Sequence Alignment more info	PubMed
acts_upstream_of_or_within establishment of epithelial cell apical/basal polarity	ISA Inferred from Sequence Alignment more info	PubMed
involved_in positive regulation of cell junction assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to plasma membrane	IEA Inferred from Electronic Annotation more info
involved_in protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in apical part of cell	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	ISA Inferred from Sequence Alignment more info	PubMed
located_in bicellular tight junction	IEA Inferred from Electronic Annotation more info
located_in cell junction	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
part_of subapical complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: protein crumbs homolog 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001347408.2 → NP_001334337.1 protein crumbs homolog 3 isoform 2

Status: VALIDATED

Source sequence(s)

CT571247

Consensus CDS

CCDS84327.1

UniProtKB/Swiss-Prot

Q8QZT4

Related

ENSMUSP00000125760.2, ENSMUST00000169543.8

Conserved Domains (1) summary

pfam15345
Location:2 → 109

TMEM51; Transmembrane protein 51
NM_001359813.2 → NP_001346742.1 protein crumbs homolog 3 isoform 1 precursor

Status: VALIDATED

Source sequence(s)

CT571247

Consensus CDS

CCDS28924.1

UniProtKB/Swiss-Prot

Q3TJV6, Q8QZT4

Related

ENSMUSP00000071729.4, ENSMUST00000071826.10
NM_001359814.1 → NP_001346743.1 protein crumbs homolog 3 isoform 2

Status: VALIDATED

Source sequence(s)

CT571247

Consensus CDS

CCDS84327.1

Conserved Domains (1) summary

pfam15345
Location:2 → 109

TMEM51; Transmembrane protein 51
NM_001431884.1 → NP_001418813.1 protein crumbs homolog 3 isoform 2

Status: VALIDATED

Source sequence(s)

CT571247
NM_001431885.1 → NP_001418814.1 protein crumbs homolog 3 isoform 1 precursor

Status: VALIDATED

Source sequence(s)

CT571247

UniProtKB/Swiss-Prot

Q3TJV6, Q8QZT4
NM_177638.6 → NP_808306.1 protein crumbs homolog 3 isoform 1 precursor

See identical proteins and their annotated locations for NP_808306.1

Status: VALIDATED

Source sequence(s)

CT571247

Consensus CDS

CCDS28924.1

UniProtKB/Swiss-Prot

Q3TJV6, Q8QZT4

Related

ENSMUSP00000094902.4, ENSMUST00000097299.10