Hs2st1 heparan sulfate 2-O-sulfotransferase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Hs2st1provided by MGI
Official Full Name: heparan sulfate 2-O-sulfotransferase 1provided by MGI
Primary source: MGI:MGI:1346049
See related: Ensembl:ENSMUSG00000040151 AllianceGenome:MGI:1346049
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 2OST; Hs2st; mKIAA0448
Summary: Enables heparan sulfate 2-O-sulfotransferase activity. Involved in heparan sulfate proteoglycan biosynthetic process. Acts upstream of or within several processes, including heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process; heparin metabolic process; and ureteric bud formation. Is active in Golgi apparatus. Is expressed in brain and future brain. Orthologous to human HS2ST1 (heparan sulfate 2-O-sulfotransferase 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in lung adult (RPKM 8.3), whole brain E14.5 (RPKM 6.1) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 H2; 3 68.89 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (144135462..144275977, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (144429701..144570216, complement)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Chondrocytes respond to an altered heparan sulfate composition with distinct changes of heparan sulfate structure and increased levels of chondroitin sulfate.
Title: Chondrocytes respond to an altered heparan sulfate composition with distinct changes of heparan sulfate structure and increased levels of chondroitin sulfate.
The specific sulfation modification of the cell surface and extracellular carbohydrate heparan sulfate (HS) performed by Hs2st suppresses the supply of translocation signals to astroglial precursors by a non-cell-autonomous mechanism. Hs2st modification selectively facilitates a physical interaction between Fgf17 and HS and suppresses Fgf17 protein levels in vivo, suggesting that Hs2st acts selectively on Fgf17 signaling.
Title: Heparan Sulfate Sulfation by Hs2st Restricts Astroglial Precursor Somal Translocation in Developing Mouse Forebrain by a Non-Cell-Autonomous Mechanism.
Inactivation of uronyl 2-O-sulfotransferase in endothelial cells (Hs2st), an enzyme that acts downstream from Ndst1, results in enhanced neutrophil recruitment in several models of acute inflammation.
Title: Inactivation of heparan sulfate 2-O-sulfotransferase accentuates neutrophil infiltration during acute inflammation in mice.
Plasma lipoprotein clearance depends on specific subclasses of sulfate groups and not on overall charge of the chains.
Title: Heparan sulfate 2-O-sulfotransferase is required for triglyceride-rich lipoprotein clearance.
mice homozygous for a null mutation in Hs2st indicated reduction in the thickness of the embryonic cerebral cortex; critical role for 2-O-sulphation of heparan sulphate proteoglycan in regulating cell proliferation during cerebral cortex development
Title: Specific modification of heparan sulphate is required for normal cerebral cortical development.
Hs2st and/or Hs6st1 expression coincides with Slit expression domains at locations where retinal ganglion cell(RGC) axons make navigation errors in Hs2st-/- and Hs6st1-/- mutants, and Hs6st1-/-RGC axons are less sensitive to Slit2 repulsion.
Title: Heparan sulphation patterns generated by specific heparan sulfotransferase enzymes direct distinct aspects of retinal axon guidance at the optic chiasm.
The phenotype of Hs2st(-/-) mutant kidneys suggests that signalling between two tissues, ureteric bud and metanephric mesenchyme, is disrupted.
Title: Heparan sulfate 2-O-sulfotransferase (Hs2st) and mouse development.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Gene trapped (1) 1 citation
Targeted (2) 1 citation

General gene information

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Markers

AF060178 (e-PCR)
- UniSTS:178946

AW214369 (e-PCR)
- UniSTS:178947

NoName (e-PCR)
- UniSTS:234839

Hs2st1 (e-PCR), detects polymorphism
- UniSTS:463452

PMC316906P1 (e-PCR)
- UniSTS:273057

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables heparan sulfate 2-O-sulfotransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables heparan sulfate 2-O-sulfotransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables heparan sulfate 2-O-sulfotransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables heparan sulfate 2-O-sulfotransferase activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heparan sulfate proteoglycan biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heparan sulfate proteoglycan biosynthetic process, enzymatic modification	IBA Inferred from Biological aspect of Ancestor more info
involved_in heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heparin metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within ureteric bud formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: heparan sulfate 2-O-sulfotransferase 1

Names: 2-O-sulfotransferase

NP_001342163.1

EC 2.8.2.-

NP_035958.3

EC 2.8.2.-

XP_006501513.1

EC 2.8.2.-

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001355234.1 → NP_001342163.1 heparan sulfate 2-O-sulfotransferase 1 isoform 1

Status: VALIDATED

Source sequence(s)

AC123880, AC159976, BC059008

Conserved Domains (1) summary

pfam03567
Location:76 → 327

Sulfotransfer_2; Sulfotransferase family
NM_011828.4 → NP_035958.3 heparan sulfate 2-O-sulfotransferase 1 isoform 2

See identical proteins and their annotated locations for NP_035958.3

Status: VALIDATED

Source sequence(s)

AC123880, AC159976, BC059008

Consensus CDS

CCDS17883.1

UniProtKB/Swiss-Prot

O88464, Q3TNP7, Q8R3H7, Q9JLK2

Related

ENSMUSP00000043066.8, ENSMUST00000043325.9

Conserved Domains (1) summary

pfam03567
Location:76 → 326

Sulfotransfer_2; Sulfotransferase family

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

144135462..144275977 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006501450.5 → XP_006501513.1 heparan sulfate 2-O-sulfotransferase 1 isoform X1

Conserved Domains (1) summary

pfam03567
Location:18 → 269

Sulfotransfer_2; Sulfotransferase family