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Mid2 midline 2 [ Mus musculus (house mouse) ]

Gene ID: 23947, updated on 27-Nov-2024

Summary

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Official Symbol
Mid2provided by MGI
Official Full Name
midline 2provided by MGI
Primary source
MGI:MGI:1344333
See related
Ensembl:ENSMUSG00000000266 AllianceGenome:MGI:1344333
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
FXY2; Trim1
Summary
Predicted to enable several functions, including microtubule binding activity; protein homodimerization activity; and transcription coactivator activity. Predicted to be involved in several processes, including defense response to symbiont; negative regulation of viral transcription; and positive regulation of NF-kappaB transcription factor activity. Predicted to be located in cytoplasm. Predicted to colocalize with microtubule. Is expressed in several structures, including alimentary system; branchial arch; central nervous system; heart; and sensory organ. Human ortholog(s) of this gene implicated in non-syndromic X-linked intellectual disability 101. Orthologous to human MID2 (midline 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Ubiquitous expression in lung adult (RPKM 6.4), bladder adult (RPKM 4.0) and 26 other tissues See more
Orthologs
human all
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Genomic context

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See Mid2 in Genome Data Viewer
Location:
X F1; X 61.35 cM
Exon count:
11
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) X NC_000086.8 (139565326..139668464)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) X NC_000086.7 (140664304..140767715)

Chromosome X - NC_000086.8Genomic Context describing neighboring genes Neighboring gene TSC22 domain family, member 3 Neighboring gene glycine cleavage system protein H (aminomethyl carrier) pseudogene Neighboring gene predicted gene, 23199 Neighboring gene eukaryotic translation initiation factor 2C, 5 Neighboring gene eukaryotic translation initiation factor 2C, 2 pseudogene

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. MID2 can substitute for MID1 and control exocytosis of lytic granules in cytotoxic T cells. Boding L, et al. APMIS, 2015 Aug. PMID 25924778
  2. Mid1/Mid2 expression in craniofacial development and a literature review of X-linked opitz syndrome. Li B, et al. Mol Genet Genomic Med, 2016 Jan. PMID 26788540, Free PMC Article
  3. FXY2/MID2, a gene related to the X-linked Opitz syndrome gene FXY/MID1, maps to Xq22 and encodes a FNIII domain-containing protein that associates with microtubules. Perry J, et al. Genomics, 1999 Dec 15. PMID 10644436
  4. MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development. Buchner G, et al. Hum Mol Genet, 1999 Aug. PMID 10400986
  5. The X-Linked-Intellectual-Disability-Associated Ubiquitin Ligase Mid2 Interacts with Astrin and Regulates Astrin Levels to Promote Cell Division. Gholkar AA, et al. Cell Rep, 2016 Jan 12. PMID 26748699, Free PMC Article

See all (20) citations in PubMed

GeneRIFs: Gene References Into Functions

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (2) 
  • Targeted (1) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables enzyme binding ISO
Inferred from Sequence Orthology
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables microtubule binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables microtubule binding ISO
Inferred from Sequence Orthology
more info
  
enables phosphoprotein binding ISO
Inferred from Sequence Orthology
more info
  
enables protein homodimerization activity ISO
Inferred from Sequence Orthology
more info
  
enables transcription coactivator activity ISO
Inferred from Sequence Orthology
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in innate immune response ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of viral entry into host cell ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of viral transcription ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of DNA-binding transcription factor activity ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of NF-kappaB transcription factor activity ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of autophagy ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of autophagy ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in positive regulation of canonical NF-kappaB signal transduction ISO
Inferred from Sequence Orthology
more info
  
involved_in protein localization to microtubule IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein localization to microtubule ISO
Inferred from Sequence Orthology
more info
  
involved_in protein ubiquitination IEA
Inferred from Electronic Annotation
more info
  
involved_in suppression of viral release by host ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
colocalizes_with microtubule IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in microtubule IEA
Inferred from Electronic Annotation
more info
  
colocalizes_with microtubule ISO
Inferred from Sequence Orthology
more info
  
located_in microtubule ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
probable E3 ubiquitin-protein ligase MID2
Names
RING-type E3 ubiquitin transferase MID2
midline defect 2
tripartite motif-containing protein 1
NP_001345295.1
NP_001345296.1
NP_001390295.1
NP_035975.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001358366.2 → NP_001345295.1  probable E3 ubiquitin-protein ligase MID2 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AL683809, BX470203
    UniProtKB/Swiss-Prot
    Q9QUS6
    UniProtKB/TrEMBL
    B1AVF5
    Related
    ENSMUSP00000108614.2, ENSMUST00000112990.8
    Conserved Domains (5) summary
    smart00502
    Location:239 → 364
    BBC; B-Box C-terminal domain
    smart00336
    Location:195 → 232
    BBOX; B-Box-type zinc finger
    cd00063
    Location:401 → 501
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    cd13739
    Location:506 → 675
    SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2
    cd16754
    Location:30 → 82
    RING-HC_MID2; RING finger, HC subclass, found in midline-2 (MID2) and similar proteins

  2. NM_001358367.2 → NP_001345296.1  probable E3 ubiquitin-protein ligase MID2 isoform 1

    Status: VALIDATED

    Source sequence(s)
    AL683809, BX470203
    UniProtKB/TrEMBL
    B1AVF5
    Related
    ENSMUSP00000108612.2, ENSMUST00000112988.8
    Conserved Domains (5) summary
    smart00502
    Location:239 → 364
    BBC; B-Box C-terminal domain
    smart00336
    Location:195 → 232
    BBOX; B-Box-type zinc finger
    cd00063
    Location:401 → 531
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    cd13739
    Location:536 → 705
    SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2
    cd16754
    Location:30 → 82
    RING-HC_MID2; RING finger, HC subclass, found in midline-2 (MID2) and similar proteins

  3. NM_001403366.1 → NP_001390295.1  probable E3 ubiquitin-protein ligase MID2 isoform 4

    Status: VALIDATED

    Source sequence(s)
    AL683809, BX470203

  4. NM_011845.4 → NP_035975.1  probable E3 ubiquitin-protein ligase MID2 isoform 3

    See identical proteins and their annotated locations for NP_035975.1

    Status: VALIDATED

    Source sequence(s)
    AL683809, BX470203
    Consensus CDS
    CCDS41151.1
    UniProtKB/TrEMBL
    B1AVF4, B1AVF5
    Related
    ENSMUSP00000108617.2, ENSMUST00000112993.2
    Conserved Domains (7) summary
    smart00502
    Location:219 → 344
    BBC; B-Box C-terminal domain
    cd00063
    Location:381 → 481
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    cd13739
    Location:486 → 655
    SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2
    cd16754
    Location:10 → 62
    RING-HC_MID2; RING finger, HC subclass, found in midline-2 (MID2) and similar proteins
    pfam18568
    Location:323 → 374
    COS; TRIM C-terminal subgroup One Signature domain
    cd19823
    Location:173 → 212
    Bbox2_MID2_C-I; B-box-type 2 zinc finger found in midline-2 (MID2) and similar proteins
    cd19837
    Location:115 → 167
    Bbox1_MID2_C-I; B-box-type 1 zinc finger found in midline-2 (MID2) and similar proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000086.8 Reference GRCm39 C57BL/6J

    Range
    139565326..139668464
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9QUS6.2 GenPept UniProtKB/Swiss-Prot:Q9QUS6

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