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Nt5e 5' nucleotidase, ecto [ Mus musculus (house mouse) ]

Gene ID: 23959, updated on 27-Nov-2024

Summary

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Official Symbol
Nt5eprovided by MGI
Official Full Name
5' nucleotidase, ectoprovided by MGI
Primary source
MGI:MGI:99782
See related
Ensembl:ENSMUSG00000032420 AllianceGenome:MGI:99782
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
NT; Nt5; eNT; CD73; 5'-NT; 2210401F01Rik
Summary
This gene encodes a membrane-bound nucleotidase that hydrolyzes extracellular nucleoside monophosphates. The encoded preproprotein undergoes proteolytic processing to generate to a functional, homodimeric enzyme that preferentially uses adenosine monophosphate as a substrate to generate free adenosine. Mice lacking the encoded protein exhibit a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate in response to a saturating increase of tubular perfusion flow. [provided by RefSeq, Sep 2016]
Expression
Broad expression in bladder adult (RPKM 18.6), lung adult (RPKM 11.8) and 21 other tissues See more
Orthologs
human all
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Genomic context

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See Nt5e in Genome Data Viewer
Location:
9 E3.1; 9 47.24 cM
Exon count:
9
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 9 NC_000075.7 (88209662..88254142)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 9 NC_000075.6 (88327609..88372089)

Chromosome 9 - NC_000075.7Genomic Context describing neighboring genes Neighboring gene solute carrier family 25, member 40 pseudogene Neighboring gene STARR-positive B cell enhancer ABC_E8304 Neighboring gene STARR-seq mESC enhancer starr_24855 Neighboring gene microRNA 12205 Neighboring gene sorting nexin 14 Neighboring gene STARR-positive B cell enhancer ABC_E10573 Neighboring gene 60S ribosomal protein L21 pseudogene Neighboring gene STARR-seq mESC enhancer starr_24856 Neighboring gene synaptotagmin binding, cytoplasmic RNA interacting protein Neighboring gene STARR-positive B cell enhancer ABC_E9772

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Extracellular adenosine produced by ecto-5'-nucleotidase (CD73) regulates macrophage pro-inflammatory responses, nitric oxide production, and favors Salmonella persistence. Costales MG, et al. Nitric Oxide, 2018 Jan 30. PMID 29108754
  2. CD73-generated adenosine facilitates Toxoplasma gondii differentiation to long-lived tissue cysts in the central nervous system. Mahamed DA, et al. Proc Natl Acad Sci U S A, 2012 Oct 2. PMID 22988118, Free PMC Article
  3. Lack of ecto-5'-nucleotidase (CD73) promotes arteriogenesis. Böring YC, et al. Cardiovasc Res, 2013 Jan 1. PMID 22977005
  4. Adenosine Formed by CD73 on T Cells Inhibits Cardiac Inflammation and Fibrosis and Preserves Contractile Function in Transverse Aortic Constriction-Induced Heart Failure. Quast C, et al. Circ Heart Fail, 2017 Apr. PMID 28404626
  5. Inhibition of CD73 improves B cell-mediated anti-tumor immunity in a mouse model of melanoma. Forte G, et al. J Immunol, 2012 Sep 1. PMID 22826317, Free PMC Article

See all (247) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Intrinsic ecto-5'-Nucleotidase/A1R Coupling may Confer Neuroprotection to the Cerebellum in Experimental Autoimmune Encephalomyelitis.
    Title: Intrinsic ecto-5'-Nucleotidase/A(1)R Coupling may Confer Neuroprotection to the Cerebellum in Experimental Autoimmune Encephalomyelitis.
  2. CD73 induces GM-CSF/MDSC-mediated suppression of T cells to accelerate pancreatic cancer pathogenesis.
    Title: CD73 induces GM-CSF/MDSC-mediated suppression of T cells to accelerate pancreatic cancer pathogenesis.
  3. Protective role of the CD73-A2AR axis in cirrhotic cardiomyopathy through negative feedback regulation of the NF-kappaB pathway.
    Title: Protective role of the CD73-A2AR axis in cirrhotic cardiomyopathy through negative feedback regulation of the NF-κB pathway.
  4. Enhanced immunosuppressive capability of mesenchymal stem cell-derived small extracellular vesicles with high expression of CD73 in experimental autoimmune uveitis.
    Title: Enhanced immunosuppressive capability of mesenchymal stem cell-derived small extracellular vesicles with high expression of CD73 in experimental autoimmune uveitis.
  5. Cdc73 protects Notch-induced T-cell leukemia cells from DNA damage and mitochondrial stress.
    Title: Cdc73 protects Notch-induced T-cell leukemia cells from DNA damage and mitochondrial stress.
  6. Immune cell expression patterns of CD39/CD73 ectonucleotidases in rodent models of cardiac arrest and resuscitation.
    Title: Immune cell expression patterns of CD39/CD73 ectonucleotidases in rodent models of cardiac arrest and resuscitation.
  7. CD73/adenosine axis exerts cardioprotection against hypobaric hypoxia-induced metabolic shift and myocarditis in a sex-dependent manner.
    Title: CD73/adenosine axis exerts cardioprotection against hypobaric hypoxia-induced metabolic shift and myocarditis in a sex-dependent manner.
  8. CD73-dependent generation of extracellular adenosine by vascular endothelial cells modulates de novo lipogenesis in adipose tissue.
    Title: CD73-dependent generation of extracellular adenosine by vascular endothelial cells modulates de novo lipogenesis in adipose tissue.
  9. Role of ecto-5'-nucleotidase in bladder function.
    Title: Role of ecto-5'-nucleotidase in bladder function.
  10. CD73 Blockade Alleviated Hepatic Fibrosis via Inhibiting Hepatic Stellate Cells Proliferation and Activation.
    Title: CD73 Blockade Alleviated Hepatic Fibrosis via Inhibiting Hepatic Stellate Cells Proliferation and Activation.
Submit: New GeneRIF Correction See all GeneRIFs (153)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (8) 
  • Targeted (6)  1 citation

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables 5'-deoxynucleotidase activity IEA
Inferred from Electronic Annotation
more info
  
enables 5'-deoxynucleotidase activity ISO
Inferred from Sequence Orthology
more info
  
enables 5'-nucleotidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 5'-nucleotidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 5'-nucleotidase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables 5'-nucleotidase activity ISO
Inferred from Sequence Orthology
more info
 PubMed 
enables GMP 5'-nucleotidase activity IEA
Inferred from Electronic Annotation
more info
  
enables GMP 5'-nucleotidase activity ISO
Inferred from Sequence Orthology
more info
  
enables IMP 5'-nucleotidase activity IEA
Inferred from Electronic Annotation
more info
  
enables IMP 5'-nucleotidase activity ISO
Inferred from Sequence Orthology
more info
  
enables XMP 5'-nucleosidase activity IEA
Inferred from Electronic Annotation
more info
  
enables ferrous iron binding ISO
Inferred from Sequence Orthology
more info
  
enables identical protein binding IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables thymidylate 5'-phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables thymidylate 5'-phosphatase activity ISO
Inferred from Sequence Orthology
more info
  
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in ADP catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in AMP catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within AMP catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in AMP catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in AMP catabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in ATP metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within adenosine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in adenosine biosynthetic process ISO
Inferred from Sequence Orthology
more info
  
involved_in adenosine metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in calcium ion homeostasis IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in inhibition of non-skeletal tissue mineralization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in inhibition of non-skeletal tissue mineralization ISO
Inferred from Sequence Orthology
more info
 PubMed 
involved_in leukocyte cell-cell adhesion IEA
Inferred from Electronic Annotation
more info
  
involved_in leukocyte cell-cell adhesion ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within negative regulation of inflammatory response IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in response to ATP IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cell surface ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
is_active_in external side of plasma membrane IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane ISO
Inferred from Sequence Orthology
more info
  
located_in membrane ISS
Inferred from Sequence or Structural Similarity
more info
  
located_in nucleoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in plasma membrane ISO
Inferred from Sequence Orthology
more info
 PubMed 
located_in plasma membrane ISO
Inferred from Sequence Orthology
more info
  
located_in synaptic membrane ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
5'-nucleotidase
Names
5'-deoxynucleotidase
IMP-specific 5'-nucleotidase
ecto-5'-nucleotidase
thymidylate 5'-phosphatase
NP_035981.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_011851.4 → NP_035981.1  5'-nucleotidase preproprotein

    See identical proteins and their annotated locations for NP_035981.1

    Status: REVIEWED

    Source sequence(s)
    AK029979, BB850214
    Consensus CDS
    CCDS23386.1
    UniProtKB/Swiss-Prot
    Q3U3S1, Q61503
    UniProtKB/TrEMBL
    Q0VEE0
    Related
    ENSMUSP00000034992.7, ENSMUST00000034992.8
    Conserved Domains (3) summary
    COG0737
    Location:21 → 534
    UshA; 2',3'-cyclic-nucleotide 2'-phosphodiesterase/5'- or 3'-nucleotidase, 5'-nucleotidase family [Nucleotide transport and metabolism, Defense mechanisms]
    cd07409
    Location:31 → 312
    MPP_CD73_N; CD73 ecto-5'-nucleotidase and related proteins, N-terminal metallophosphatase domain
    pfam02872
    Location:354 → 509
    5_nucleotid_C; 5'-nucleotidase, C-terminal domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000075.7 Reference GRCm39 C57BL/6J

    Range
    88209662..88254142
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q61503.2 GenPept UniProtKB/Swiss-Prot:Q61503

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