Raf1 Raf-1 proto-oncogene, serine/threonine kinase [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Raf1provided by RGD
Official Full Name: Raf-1 proto-oncogene, serine/threonine kinaseprovided by RGD
Primary source: RGD:3531
See related: EnsemblRapid:ENSRNOG00000010153 AllianceGenome:RGD:3531
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables several functions, including ATP binding activity; adenylate cyclase activator activity; and enzyme binding activity. Involved in several processes, including MAPK cascade; protein phosphorylation; and response to hypoxia. Located in cytoplasm and plasma membrane. Biomarker of pancreatic carcinoma. Human ortholog(s) of this gene implicated in Noonan syndrome (multiple); Noonan syndrome with multiple lentigines 2; adenocarcinoma (multiple); and dilated cardiomyopathy 1NN. Orthologous to human RAF1 (Raf-1 proto-oncogene, serine/threonine kinase). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Muscle (RPKM 405.4), Heart (RPKM 314.2) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 4q42

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	4	NC_086022.1 (150352158..150412813, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	4	NC_051339.1 (148679534..148740265, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	4	NC_005103.4 (147532040..147592769, complement)

Chromosome 4 - NC_086022.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Raf1 interacts with OIP5 to participate in oxaliplatin-induced neuropathic pain.
Title: Raf1 interacts with OIP5 to participate in oxaliplatin-induced neuropathic pain.
Maf1 Ameliorates Sepsis-Associated Encephalopathy by Suppressing the NF-kB/NLRP3 Inflammasome Signaling Pathway.
Title: Maf1 Ameliorates Sepsis-Associated Encephalopathy by Suppressing the NF-kB/NLRP3 Inflammasome Signaling Pathway.
O-GlcNAcylation suppressed the ubiquitination of RAF1.
Title: O-GlcNAcylation of RAF1 increases its stabilization and induces the renal fibrosis.
Wild-type PKGII phosphorylated Raf-1 at Ser-43 in a cGMP-dependent manner, but a PKGII D412A/R415A mutant, which has a low affinity for cGMP, did not.
Title: Type II cGMP-dependent protein kinase negatively regulates fibroblast growth factor signaling by phosphorylating Raf-1 at serine 43 in rat chondrosarcoma cells.
Raf-1 kinase contributes to cerebral vasospasm and early brain injury after subarachnoid hemorrhage by enhancing the activation of the Raf-1/ERK1/2 and Raf-1/NF-kappaB signaling pathways.
Title: Possible Role of Raf-1 Kinase in the Development of Cerebral Vasospasm and Early Brain Injury After Experimental Subarachnoid Hemorrhage in Rats.
sorafenib exhibits a regulatory role on the occurrence of LVH following AB in rats by blocking the rise in growth factors PDGF-BB and TGFbeta1, activation of the corresponding c-Raf-ERK1/2 signaling pathway
Title: Multikinase inhibitor sorafenib prevents pressure overload-induced left ventricular hypertrophy in rats by blocking the c-Raf/ERK1/2 signaling pathway.
Our results demonstrate that XIAP plays a new role as a negative regulator of neurotrophin-induced neurite outgrowth and neuronal differentiation in developing neurons.
Title: X-linked inhibitor of apoptosis protein negatively regulates neuronal differentiation through interaction with cRAF and Trk.
Data indicate that Ras-Raf-ERK-Sp1 signaling pathway is responsibe for FGF-2-induced nestin expression.
Title: Fibroblast growth factor-2 up-regulates the expression of nestin through the Ras-Raf-ERK-Sp1 signaling axis in C6 glioma cells.
Induction of streptozotocin diabetes resulted in decreased level of GLP-1R and increased levels of IRS-1 and Raf-1 in the liver.
Title: Effects of selenium and exendin-4 on glucagon-like peptide-1 receptor, IRS-1, and Raf-1 in the liver of diabetic rats.
Data show that a dimeric Raf kinase inhibitor protein (RKIP) mutant displayed a higher affinity to G protein-coupled receptor kinase (GRK) 2, but a lower affinity to Raf1.
Title: Raf kinase inhibitor protein (RKIP) dimer formation controls its target switch from Raf1 to G protein-coupled receptor kinase (GRK) 2.

Submit: New GeneRIF Correction See all GeneRIFs (27)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH142970 (e-PCR)
- UniSTS:232217

RH139128 (e-PCR)
- UniSTS:221214

UniSTS:469819 (e-PCR)
- UniSTS:469819

Raf1 (e-PCR), detects polymorphism
- UniSTS:469819

BI284862 (e-PCR)
- UniSTS:244310

AA990557 (e-PCR)
- UniSTS:160660

Raf1 (e-PCR), detects polymorphism
- UniSTS:536493

Raf1 (e-PCR), detects polymorphism
- UniSTS:259519

RH127364 (e-PCR)
- UniSTS:210674

RH140847 (e-PCR)
- UniSTS:210674

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables MAP kinase kinase kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables MAP kinase kinase kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables MAP kinase kinase kinase activity	IEA Inferred from Electronic Annotation more info
enables MAP kinase kinase kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables MAP kinase kinase kinase activity	ISO Inferred from Sequence Orthology more info
enables MAP kinase kinase kinase activity	TAS Traceable Author Statement more info	PubMed
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables adenylate cyclase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables adenylate cyclase binding	IPI Inferred from Physical Interaction more info	PubMed
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables mitogen-activated protein kinase kinase binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info
enables small GTPase binding	IEA Inferred from Electronic Annotation more info
NOT enables small GTPase binding	ISO Inferred from Sequence Orthology more info
enables small GTPase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in ERBB2-ERBB3 signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in ERBB2-ERBB3 signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in MAPK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in MAPK cascade	IDA Inferred from Direct Assay more info	PubMed
involved_in MAPK cascade	IEA Inferred from Electronic Annotation more info
involved_in MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Schwann cell development	IEA Inferred from Electronic Annotation more info
involved_in Schwann cell development	ISO Inferred from Sequence Orthology more info
involved_in activation of adenylate cyclase activity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in death-inducing signaling complex assembly	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within death-inducing signaling complex assembly	ISO Inferred from Sequence Orthology more info
involved_in extrinsic apoptotic signaling pathway via death domain receptors	IEA Inferred from Electronic Annotation more info
acts_upstream_of extrinsic apoptotic signaling pathway via death domain receptors	ISO Inferred from Sequence Orthology more info
involved_in face development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within face development	ISO Inferred from Sequence Orthology more info
involved_in heart development	IEP Inferred from Expression Pattern more info	PubMed
involved_in insulin receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in insulin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in insulin secretion involved in cellular response to glucose stimulus	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within insulin secretion involved in cellular response to glucose stimulus	ISO Inferred from Sequence Orthology more info
involved_in insulin-like growth factor receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in insulin-like growth factor receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in intermediate filament cytoskeleton organization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within intermediate filament cytoskeleton organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within intracellular glucose homeostasis	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	TAS Traceable Author Statement more info	PubMed
involved_in myelination	IEA Inferred from Electronic Annotation more info
involved_in myelination	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell population proliferation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of extrinsic apoptotic signaling pathway via death domain receptors	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of extrinsic apoptotic signaling pathway via death domain receptors	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein-containing complex assembly	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of protein-containing complex assembly	ISO Inferred from Sequence Orthology more info
involved_in neurotrophin TRK receptor signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within neurotrophin TRK receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of MAPK cascade	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of MAPK cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	TAS Traceable Author Statement more info	PubMed
involved_in response to hypoxia	IDA Inferred from Direct Assay more info	PubMed
involved_in response to muscle stretch	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to muscle stretch	ISO Inferred from Sequence Orthology more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
involved_in somatic stem cell population maintenance	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within somatic stem cell population maintenance	ISO Inferred from Sequence Orthology more info
involved_in thymus development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within thymus development	ISO Inferred from Sequence Orthology more info
involved_in thyroid gland development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within thyroid gland development	ISO Inferred from Sequence Orthology more info
involved_in type B pancreatic cell proliferation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_positive_effect type B pancreatic cell proliferation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in pseudopodium	IEA Inferred from Electronic Annotation more info
located_in pseudopodium	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: RAF proto-oncogene serine/threonine-protein kinase

Names: C-RAF; cRaf; murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV); proto-oncogene c-RAF; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-leukemia viral oncogene 1

NP_036771.1

EC 2.7.11.1

XP_008761439.1

EC 2.7.11.1

XP_008761440.1

EC 2.7.11.1

XP_038962984.1

EC 2.7.11.1

XP_038962985.1

EC 2.7.11.1

XP_063141628.1

EC 2.7.11.1

XP_063141629.1

EC 2.7.11.1

XP_063141630.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_012639.2 → NP_036771.1 RAF proto-oncogene serine/threonine-protein kinase

See identical proteins and their annotated locations for NP_036771.1

Status: PROVISIONAL

Source sequence(s)

BC062071

UniProtKB/Swiss-Prot

P11345

UniProtKB/TrEMBL

A0A8L2Q6F4, A6IKZ0

Related

ENSRNOP00000013831.7, ENSRNOT00000013831.8

Conserved Domains (3) summary

cd14149
Location:336 → 618

STKc_C-Raf; Catalytic domain of the Serine/Threonine Kinase, C-Raf (Rapidly Accelerated Fibrosarcoma) kinase

cd17135
Location:55 → 131

RBD_CRAF; Ras-binding domain (RBD) found in RAF proto-oncogene serine/threonine-protein kinase RAF1/CRAF

cd20870
Location:136 → 187

C1_A_C-Raf; protein kinase C conserved region 1 (C1 domain) found in A- and C-Raf (Rapidly Accelerated Fibrosarcoma) kinases, and similar proteins

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086022.1 Reference GRCr8

Range

150352158..150412813 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_008763217.2 → XP_008761439.1 RAF proto-oncogene serine/threonine-protein kinase isoform X1

See identical proteins and their annotated locations for XP_008761439.1

UniProtKB/TrEMBL

A0A8I5ZWZ7, A0A8L2Q6F4

Related

ENSRNOP00000082418.1, ENSRNOT00000105918.2

Conserved Domains (3) summary

cd14149
Location:356 → 638

STKc_C-Raf; Catalytic domain of the Serine/Threonine Kinase, C-Raf (Rapidly Accelerated Fibrosarcoma) kinase

cd17135
Location:55 → 131

RBD_CRAF; Ras-binding domain (RBD) found in RAF proto-oncogene serine/threonine-protein kinase RAF1/CRAF

cd20870
Location:136 → 187

C1_A_C-Raf; protein kinase C conserved region 1 (C1 domain) found in A- and C-Raf (Rapidly Accelerated Fibrosarcoma) kinases, and similar proteins
XM_063285558.1 → XP_063141628.1 RAF proto-oncogene serine/threonine-protein kinase isoform X2

UniProtKB/Swiss-Prot

P11345

UniProtKB/TrEMBL

A6IKZ0
XM_039107056.2 → XP_038962984.1 RAF proto-oncogene serine/threonine-protein kinase isoform X1

UniProtKB/TrEMBL

A0A8I5ZWZ7, A0A8L2Q6F4

Conserved Domains (3) summary

cd14149
Location:356 → 638

STKc_C-Raf; Catalytic domain of the Serine/Threonine Kinase, C-Raf (Rapidly Accelerated Fibrosarcoma) kinase

cd17135
Location:55 → 131

RBD_CRAF; Ras-binding domain (RBD) found in RAF proto-oncogene serine/threonine-protein kinase RAF1/CRAF

cd20870
Location:136 → 187

C1_A_C-Raf; protein kinase C conserved region 1 (C1 domain) found in A- and C-Raf (Rapidly Accelerated Fibrosarcoma) kinases, and similar proteins
XM_008763218.4 → XP_008761440.1 RAF proto-oncogene serine/threonine-protein kinase isoform X1

See identical proteins and their annotated locations for XP_008761440.1

UniProtKB/TrEMBL

A0A8I5ZWZ7, A0A8L2Q6F4

Conserved Domains (3) summary

cd14149
Location:356 → 638

STKc_C-Raf; Catalytic domain of the Serine/Threonine Kinase, C-Raf (Rapidly Accelerated Fibrosarcoma) kinase

cd17135
Location:55 → 131

RBD_CRAF; Ras-binding domain (RBD) found in RAF proto-oncogene serine/threonine-protein kinase RAF1/CRAF

cd20870
Location:136 → 187

C1_A_C-Raf; protein kinase C conserved region 1 (C1 domain) found in A- and C-Raf (Rapidly Accelerated Fibrosarcoma) kinases, and similar proteins
XM_039107057.2 → XP_038962985.1 RAF proto-oncogene serine/threonine-protein kinase isoform X2

UniProtKB/Swiss-Prot

P11345

UniProtKB/TrEMBL

A0A8L2Q6F4, A6IKZ0

Conserved Domains (3) summary

cd14149
Location:336 → 618

STKc_C-Raf; Catalytic domain of the Serine/Threonine Kinase, C-Raf (Rapidly Accelerated Fibrosarcoma) kinase

cd17135
Location:55 → 131

RBD_CRAF; Ras-binding domain (RBD) found in RAF proto-oncogene serine/threonine-protein kinase RAF1/CRAF

cd20870
Location:136 → 187

C1_A_C-Raf; protein kinase C conserved region 1 (C1 domain) found in A- and C-Raf (Rapidly Accelerated Fibrosarcoma) kinases, and similar proteins
XM_063285560.1 → XP_063141630.1 RAF proto-oncogene serine/threonine-protein kinase isoform X4
XM_063285559.1 → XP_063141629.1 RAF proto-oncogene serine/threonine-protein kinase isoform X3