bub1 mitotic spindle checkpoint kinase Bub1 [Schizosaccharomyces pombe (fission yeast)] - Gene

Summary

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Official Symbol: bub1
Official Full Name: mitotic spindle checkpoint kinase Bub1
Primary source: PomBase:SPCC1322.12c
Locus tag: SPOM_SPCC1322.12C
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Schizosaccharomyces pombe (strain: 972h-)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
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Genomic context

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See bub1 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 1

Sequence:: Chromosome: III; NC_003421.2 (1312560..1315997, complement)

Chromosome III - NC_003421.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003421.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phosphorylation of Bub1 by Mph1 promotes Bub1 signaling at the kinetochore to ensure accurate chromosome segregation.
Title: Phosphorylation of Bub1 by Mph1 promotes Bub1 signaling at the kinetochore to ensure accurate chromosome segregation.
The Bub1 TPR domain is both necessary and sufficient to bind and recruit Mad3.
Title: The Bub1-TPR Domain Interacts Directly with Mad3 to Generate Robust Spindle Checkpoint Arrest.
Study shows that fission yeast Moa1 (meikin), which associates stably with CENP-C during meiosis I, recruits Plo1 (polo-like kinase) to the kinetochores and phosphorylates Spc7 (KNL1) to accumulate Bub1. Consequently, in contrast to the transient kinetochore localization of mitotic Bub1, meiotic Bub1 persists at kinetochores until anaphase I.
Title: Meikin-associated polo-like kinase specifies Bub1 distribution in meiosis I.
findings show phosphorylation of histone H3-threonine 3 mediated by Haspin cooperates with Bub1-mediated histone 2A-serine 121 phosphorylation in targeting the chromosomal passenger complex to the inner centromere in fission yeast and human cells
Title: Two histone marks establish the inner centromere and chromosome bi-orientation.
findings show that Bub1 phosphorylates the conserved serine 121 of histone H2A; Bub1 kinase creates a mark for shugoshin localization and the correct partitioning of chromosomes
Title: Phosphorylation of H2A by Bub1 prevents chromosomal instability through localizing shugoshin.
find that Bub3 is also dispensable for shugoshin localization to the centromeres, which is the second known function of Bub1.
Title: Bub1 and Bub3 promote the conversion from monopolar to bipolar chromosome attachment independently of shugoshin.
analysis of dual regulation of Mad2 localization on kinetochores by Bub1 and Dam1/DASH that ensure proper spindle interaction
Title: Dual regulation of Mad2 localization on kinetochores by Bub1 and Dam1/DASH that ensure proper spindle interaction.
Bub1 stably binds kinetochores, and by tethering Bub1 to telomeres we demonstrate that it is sufficient to recruit anaphase inhibitors in a kinase-independent manner. We propose that the major checkpoint role for Bub1 is as a signalling scaffold
Title: Bub1 is a fission yeast kinetochore scaffold protein, and is sufficient to recruit other spindle checkpoint proteins to ectopic sites on chromosomes.
Sgo2, which assists in loading Aurora to centromeres, and its regulator Bub1 are required for the mono-orientation of sister chromatids in meiosis-I, while Ark1 is required for the faithful bi-orientation
Title: Aurora controls sister kinetochore mono-orientation and homolog bi-orientation in meiosis-I.
The N terminus of Bub1 is necessary and sufficient for Sgo1 targeting to centromeres and the protection of cohesion during meiosis.
Title: Control of Shugoshin function during fission-yeast meiosis.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by PomBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in homologous chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiosis I spindle assembly checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic centromeric cohesion protection in anaphase I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic sister chromatid cohesion, centromeric	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic sister chromatid biorientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle assembly checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle assembly checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of bub1-bub3 complex	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in chromosome, centromeric core domain	IDA Inferred from Direct Assay more info	PubMed
is_active_in chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
part_of inner kinetochore	EXP Inferred from Experiment more info	PubMed
is_active_in kinetochore	IBA Inferred from Biological aspect of Ancestor more info
is_active_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitotic spindle pole body	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed
is_active_in old mitotic spindle pole body	IDA Inferred from Direct Assay more info	PubMed
part_of outer kinetochore	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: mitotic spindle checkpoint kinase Bub1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003421.2 Reference assembly

Range

1312560..1315997 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001023130.3 → NP_588140.1 mitotic spindle checkpoint kinase Bub1 [Schizosaccharomyces pombe]

See identical proteins and their annotated locations for NP_588140.1

Status: PROVISIONAL

UniProtKB/Swiss-Prot

O94751

Conserved Domains (2) summary

smart00777
Location:30 → 158

Mad3_BUB1_I; Mad3/BUB1 hoMad3/BUB1 homology region 1

cd13981
Location:718 → 1040

STKc_Bub1_BubR1; Catalytic domain of the Serine/Threonine kinases, Spindle assembly checkpoint proteins Bub1 and BubR1