mus81 Holliday junction resolvase subunit Mus81 [Schizosaccharomyces pombe (fission yeast)] - Gene

Summary

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Official Symbol: mus81
Official Full Name: Holliday junction resolvase subunit Mus81
Primary source: PomBase:SPCC4G3.05c
Locus tag: SPOM_SPCC4G3.05C
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Schizosaccharomyces pombe (strain: 972h-)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
Also known as: slx3
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Genomic context

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See mus81 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 2

Sequence:: Chromosome: III; NC_003421.2 (462720..464714)

Chromosome III - NC_003421.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003421.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mutations in Mus81 and Eme1 endonuclease prevent methylation of Psm1 at K536 and K1200.
Title: Mutations that prevent methylation of cohesin render sensitivity to DNA damage in S. pombe.
Removing Rhp51, the Rad51 recombinase homologue, suppresses the slowing defect of rqh1Delta mutants, but not that of mus81Delta mutant, defining an epistatic pathway in which mus81 is epistatic to rhp51 and rhp51 is epistatic to rqh1.
Title: Mus81, Rhp51(Rad51), and Rqh1 form an epistatic pathway required for the S-phase DNA damage checkpoint.
An interplay between Mus81 activity at stalled forks and the Chk1-dependent DNA damage checkpoint during S-phase when replication forks have collapsed, was identified.
Title: Cleavage of stalled forks by fission yeast Mus81/Eme1 in absence of DNA replication checkpoint.
Findings demonstrate that Mus81/Eme1 is the dedicated endonuclease that resolves sister chromatid recombination intermediates during the repair of broken replication forks.
Title: Mus81 is essential for sister chromatid recombination at broken replication forks.
The purification of active forms of recombinant Schizosaccharomyces pombe Mus81-Eme1 and Saccharomyces cerevisiae Mus81-Mms4, which display robust Holliday junction cleavage in vitro, is reported.
Title: Mus81 cleavage of Holliday junctions: a failsafe for processing meiotic recombination intermediates?
Upon S-phase arrest by acute hydroxyurea treatment, Mus81 is not required for cell viability but is essential for recovery from replication fork collapse.
Title: Replication checkpoint kinase Cds1 regulates Mus81 to preserve genome integrity during replication stress.
The endonuclease Mus81 functions predominantly in the Rhp51-independent, Rad22-dependent DNA repair pathway. Mus81 can cleave D-loops that are formed by Rad22. [mus81]
Title: DNA repair by a Rad22-Mus81-dependent pathway that is independent of Rhp51.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by PomBase

Function	Evidence Code	Pubs
enables 3'-flap endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables crossover junction DNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables crossover junction DNA endonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in double-strand break repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via break-induced replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via homologous recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic joint molecule formation	TAS Traceable Author Statement more info	PubMed
involved_in mitotic DNA replication checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic intra-S DNA damage checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic intra-S DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in postreplication repair	TAS Traceable Author Statement more info	PubMed
involved_in reciprocal meiotic recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in replication fork processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in resolution of meiotic recombination intermediates	IBA Inferred from Biological aspect of Ancestor more info
involved_in resolution of meiotic recombination intermediates	IDA Inferred from Direct Assay more info	PubMed
involved_in resolution of meiotic recombination intermediates	IGI Inferred from Genetic Interaction more info	PubMed
involved_in resolution of meiotic recombination intermediates	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Holliday junction resolvase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Holliday junction resolvase complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitochondrion	HDA more info	PubMed
is_active_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Holliday junction resolvase subunit Mus81

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003421.2 Reference assembly

Range

462720..464714

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001355879.1 → NP_001343019.1 Holliday junction resolvase subunit Mus81 [Schizosaccharomyces pombe]

Status: PROVISIONAL

UniProtKB/Swiss-Prot

P87231, Q2EEM1

Conserved Domains (1) summary

COG1948
Location:294 → 577

MUS81; ERCC4-type nuclease [Replication, recombination and repair]