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Nit1 nitrilase 1 [ Rattus norvegicus (Norway rat) ]

Gene ID: 289222, updated on 27-Nov-2024

Summary

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Official Symbol
Nit1provided by RGD
Official Full Name
nitrilase 1provided by RGD
Primary source
RGD:727821
See related
EnsemblRapid:ENSRNOG00000003881 AllianceGenome:RGD:727821
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary
The protein encoded by this gene is a member of the nitrilase enzyme superfamily. Nitrilases are nonpeptidic C-N hydrolases. In mammals, nitrilases form small molecule C-N bonds such as acid amides, carbamates and ureas. Nit1-deficient mice display phenotypes including increased cell proliferation, resistance to DNA damage, and an increased number of N-nitrosomethylbenzylamine-induced murine forestomach tumors, suggesting a tumor suppressor function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
Expression
Biased expression in Kidney (RPKM 940.4), Adrenal (RPKM 606.4) and 9 other tissues See more
Orthologs
human mouse all
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Genomic context

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See Nit1 in Genome Data Viewer
Location:
13q24
Exon count:
8
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 13 NC_086031.1 (86302334..86305770, complement)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 13 NC_051348.1 (83769888..83773324, complement)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 13 NC_005112.4 (89722370..89725806, complement)

Chromosome 13 - NC_086031.1Genomic Context describing neighboring genes Neighboring gene ubiquitin-fold modifier conjugating enzyme 1 Neighboring gene G protein subunit gamma 5, pseudogene 1 Neighboring gene death effector domain-containing Neighboring gene prefoldin subunit 2 Neighboring gene kelch domain containing 9 Neighboring gene nectin cell adhesion molecule 4

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. Functional proteomic and structural insights into molecular recognition in the nitrilase family enzymes. Barglow KT, et al. Biochemistry, 2008 Dec 23. PMID 19053248, Free PMC Article
  2. Biological functions of mammalian Nit1, the counterpart of the invertebrate NitFhit Rosetta stone protein, a possible tumor suppressor. Semba S, et al. J Biol Chem, 2006 Sep 22. PMID 16864578
  3. Nit1 is a metabolite repair enzyme that hydrolyzes deaminated glutathione. Peracchi A, et al. Proc Natl Acad Sci U S A, 2017 Apr 18. PMID 28373563, Free PMC Article
  4. Proteomic characterization of the human sperm nucleus. de Mateo S, et al. Proteomics, 2011 Jul. PMID 21630459
  5. The nitrilase superfamily: classification, structure and function. Pace HC, et al. Genome Biol, 2001. PMID 11380987, Free PMC Article

See all (9) citations in PubMed

GeneRIFs: Gene References Into Functions

General gene information

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Markers

Homology

Clone Names

  • MGC95151

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables deaminated glutathione amidase activity IEA
Inferred from Electronic Annotation
more info
  
enables deaminated glutathione amidase activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in amide catabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in amide catabolic process ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrion IEA
Inferred from Electronic Annotation
more info
  
located_in mitochondrion ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
deaminated glutathione amidase
Names
dGSH amidase
nitrilase homolog 1
NP_001076049.1
NP_001292575.1
NP_001292576.1
NP_872609.2
XP_038946439.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001082580.1 → NP_001076049.1  deaminated glutathione amidase isoform b

    See identical proteins and their annotated locations for NP_001076049.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate splice site in the 5' coding region, which results in initiation of translation from a downstream start codon compared to variant 1. It encodes isoform b, which has a shorter N-terminus compared to isoform a. Variants 2 and 4 encode the same isoform.
    Source sequence(s)
    BC087146, FM045849, JAXUCZ010000013
    UniProtKB/TrEMBL
    A0A8L2Q1A7, A6JFY4
    Related
    ENSRNOP00000096370.2, ENSRNOT00000117822.2
    Conserved Domains (1) summary
    cd07572
    Location:13 → 280
    nit; Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)

  2. NM_001305646.1 → NP_001292575.1  deaminated glutathione amidase isoform c

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. It encodes isoform c, which is shorter than isoform a.
    Source sequence(s)
    BC087146, JAXUCZ010000013
    UniProtKB/TrEMBL
    A0A8L2Q1A7
    Conserved Domains (1) summary
    cd07572
    Location:43 → 310
    nit; Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)

  3. NM_001305647.1 → NP_001292576.1  deaminated glutathione amidase isoform b

    See identical proteins and their annotated locations for NP_001292576.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) differs in the 5' UTR and uses a downstream start codon compared to variant 1. It encodes isoform b, which has a shorter N-terminus than isoform a. Variants 2 and 4 encode the same isoform.
    Source sequence(s)
    BC087146, CV073582
    UniProtKB/TrEMBL
    A0A8L2Q1A7, A6JFY4
    Conserved Domains (1) summary
    cd07572
    Location:13 → 280
    nit; Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)

  4. NM_182668.2 → NP_872609.2  deaminated glutathione amidase isoform a

    See identical proteins and their annotated locations for NP_872609.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (a).
    Source sequence(s)
    BC087146, JAXUCZ010000013
    UniProtKB/Swiss-Prot
    Q5PQK6, Q7TQ94
    UniProtKB/TrEMBL
    A0A8L2Q1A7
    Related
    ENSRNOP00000005194.6, ENSRNOT00000005194.7
    Conserved Domains (1) summary
    cd07572
    Location:49 → 316
    nit; Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086031.1 Reference GRCr8

    Range
    86302334..86305770 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_039090511.2 → XP_038946439.1  deaminated glutathione amidase isoform X1

    Conserved Domains (1) summary
    cl11424
    Location:13 → 175
    nitrilase; Nitrilase superfamily, including nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q7TQ94.2 GenPept UniProtKB/Swiss-Prot:Q7TQ94

Gene LinkOut

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