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ARHGAP35 Rho GTPase activating protein 35 [ Homo sapiens (human) ]

Gene ID: 2909, updated on 27-Nov-2024

Summary

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Official Symbol
ARHGAP35provided by HGNC
Official Full Name
Rho GTPase activating protein 35provided by HGNC
Primary source
HGNC:HGNC:4591
See related
Ensembl:ENSG00000160007 MIM:605277; AllianceGenome:HGNC:4591
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP
Summary
The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]
Expression
Broad expression in testis (RPKM 28.9), brain (RPKM 18.7) and 25 other tissues See more
Orthologs
mouse all
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Genomic context

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See ARHGAP35 in Genome Data Viewer
Location:
19q13.32
Exon count:
9
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 19 NC_000019.10 (46860997..47005077)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 19 NC_060943.1 (49686835..49830977)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 19 NC_000019.9 (47364254..47508334)

Chromosome 19 - NC_000019.10Genomic Context describing neighboring genes Neighboring gene H3K27ac hESC enhancer GRCh37_chr19:47341466-47341966 Neighboring gene small NF90 (ILF3) associated RNA E Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14851 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:47358600-47359538 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:47359539-47360475 Neighboring gene adaptor related protein complex 2 subunit sigma 1 Neighboring gene translation initiation factor IF-2-like Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10833 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10834 Neighboring gene BRD4-independent group 4 enhancer GRCh37_chr19:47466169-47467368 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:47479369-47480226 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:47480227-47481082 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:47505718-47506463 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:47506464-47507210 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:47508684-47509262 Neighboring gene ReSE screen-validated silencer GRCh37_chr19:47514658-47514871 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10835 Neighboring gene ReSE screen-validated silencer GRCh37_chr19:47533967-47534068 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:47534993-47535494 Neighboring gene neuronal PAS domain protein 1 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14852 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10836 Neighboring gene transmembrane protein 160

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. p190 RhoGAP promotes contact inhibition in epithelial cells by repressing YAP activity. Frank SR, et al. J Cell Biol, 2018 Sep 3. PMID 29934311, Free PMC Article
  2. Mitotic down-regulation of p190RhoGAP is required for the successful completion of cytokinesis. Manchinelly SAS, et al. J Biol Chem, 2010 Aug 27. PMID 20534586, Free PMC Article
  3. Rho GTPase-activating protein 35 suppresses gastric cancer metastasis by regulating cytoskeleton reorganization and epithelial-to-mesenchymal transition. Sun Y, et al. Bioengineered, 2022 Jun. PMID 35758029, Free PMC Article
  4. P190RhoGAP prevents mitotic spindle fragmentation and is required to activate Aurora A kinase at acentriolar poles. Manukyan A, et al. Chromosoma, 2018 Sep. PMID 29656322, Free PMC Article
  5. p190A RhoGAP is involved in EGFR pathways and promotes proliferation, invasion and migration in lung adenocarcinoma cells. Notsuda H, et al. Int J Oncol, 2013 Nov. PMID 24043274

See all (110) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Diverse p120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP, and Dok1.
    Title: Diverse p120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP, and Dok1.
  2. ARHGAP35 is a novel factor disrupted in human developmental eye phenotypes.
    Title: ARHGAP35 is a novel factor disrupted in human developmental eye phenotypes.
  3. Tumor-derived ARHGAP35 mutations enhance the Galpha13-Rho signaling axis in human endometrial cancer.
    Title: Tumor-derived ARHGAP35 mutations enhance the Gα(13)-Rho signaling axis in human endometrial cancer.
  4. Identification of an inhibitory domain in GTPase-activating protein p190RhoGAP responsible for masking its functional GAP domain.
    Title: Identification of an inhibitory domain in GTPase-activating protein p190RhoGAP responsible for masking its functional GAP domain.
  5. The p190 RhoGAPs, ARHGAP35, and ARHGAP5 are implicated in GnRH neuronal development: Evidence from patients with idiopathic hypogonadotropic hypogonadism, zebrafish, and in vitro GAP activity assay.
    Title: The p190 RhoGAPs, ARHGAP35, and ARHGAP5 are implicated in GnRH neuronal development: Evidence from patients with idiopathic hypogonadotropic hypogonadism, zebrafish, and in vitro GAP activity assay.
  6. Rho GTPase-activating protein 35 suppresses gastric cancer metastasis by regulating cytoskeleton reorganization and epithelial-to-mesenchymal transition.
    Title: Rho GTPase-activating protein 35 suppresses gastric cancer metastasis by regulating cytoskeleton reorganization and epithelial-to-mesenchymal transition.
  7. p190A inactivating mutations cause aberrant RhoA activation and promote malignant transformation via the Hippo-YAP pathway in endometrial cancer.
    Title: p190A inactivating mutations cause aberrant RhoA activation and promote malignant transformation via the Hippo-YAP pathway in endometrial cancer.
  8. Polycystin-1 regulates ARHGAP35-dependent centrosomal RhoA activation and ROCK signaling.
    Title: Polycystin-1 regulates ARHGAP35-dependent centrosomal RhoA activation and ROCK signaling.
  9. p190A RhoGAP induces CDH1 expression and cooperates with E-cadherin to activate LATS kinases and suppress tumor cell growth.
    Title: p190A RhoGAP induces CDH1 expression and cooperates with E-cadherin to activate LATS kinases and suppress tumor cell growth.
  10. Tau regulates the microtubule-dependent migration of glioblastoma cells via the Rho-ROCK signaling pathway.
    Title: Tau regulates the microtubule-dependent migration of glioblastoma cells via the Rho-ROCK signaling pathway.
Submit: New GeneRIF Correction See all GeneRIFs (41)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide meta-analysis identifies variants associated with platinating agent susceptibility across populations
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein Gene Interaction Pubs
Envelope transmembrane glycoprotein gp41 env The 3S motif of HIV-1 gp41 activates p190 RhoGAP PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • KIAA1722, MGC10745

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables GTP binding IEA
Inferred from Electronic Annotation
more info
  
enables GTPase activator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables GTPase activator activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables GTPase activator activity TAS
Traceable Author Statement
more info
  
enables GTPase activity IEA
Inferred from Electronic Annotation
more info
  
enables phospholipid binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in Rho protein signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in axon guidance ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in axonal fasciculation ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in camera-type eye development IEA
Inferred from Electronic Annotation
more info
  
involved_in cell migration ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in central nervous system neuron axonogenesis ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in establishment or maintenance of actin cytoskeleton polarity ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in forebrain development IEA
Inferred from Electronic Annotation
more info
  
involved_in mammary gland development ISS
Inferred from Sequence or Structural Similarity
more info
  
acts_upstream_of_or_within negative regulation of Rho protein signal transduction ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in negative regulation of vascular permeability IEA
Inferred from Electronic Annotation
more info
  
involved_in neural tube closure IEA
Inferred from Electronic Annotation
more info
  
involved_in neuron projection guidance ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in positive regulation of cilium assembly ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in positive regulation of neuron projection development ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in regulation of actin cytoskeleton organization ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in regulation of actin polymerization or depolymerization ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in regulation of axonogenesis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of axonogenesis ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in regulation of cell shape IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of cell size IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of small GTPase mediated signal transduction TAS
Traceable Author Statement
more info
  
involved_in wound healing, spreading of cells ISS
Inferred from Sequence or Structural Similarity
more info
  
Component Evidence Code Pubs
located_in actin cytoskeleton IEA
Inferred from Electronic Annotation
more info
  
located_in ciliary basal body ISS
Inferred from Sequence or Structural Similarity
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol TAS
Traceable Author Statement
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
rho GTPase-activating protein 35
Names
glucocorticoid receptor DNA-binding factor 1
glucocorticoid receptor repression factor 1
rho GAP p190A

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_047014.2 RefSeqGene

    Range
    5001..149081
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_004491.5 → NP_004482.4  rho GTPase-activating protein 35

    See identical proteins and their annotated locations for NP_004482.4

    Status: REVIEWED

    Source sequence(s)
    AB051509, AC008755, AC008895, BE871213, CA429690, CX163309
    Consensus CDS
    CCDS46127.1
    UniProtKB/Swiss-Prot
    A7E2A4, Q14452, Q9C0E1, Q9NRY4
    UniProtKB/TrEMBL
    A0A2X0SFV3
    Related
    ENSP00000500409.1, ENST00000672722.1
    Conserved Domains (5) summary
    smart00441
    Location:431 → 483
    FF; Contains two conserved F residues
    smart00173
    Location:158 → 250
    RAS; Ras subfamily of RAS small GTPases
    cd04373
    Location:1247 → 1431
    RhoGAP_p190; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p190-like proteins. p190, also named RhoGAP5, plays a role in neuritogenesis and axon branch stability. p190 shows a preference for Rho, over Rac and Cdc42, and consists of an ...
    pfam16512
    Location:261 → 340
    RhoGAP-FF1; p190-A and -B Rho GAPs FF domain
    cd22221
    Location:594 → 765
    pseudoGTPaseD_p190RhoGAP-A; pseudoGTPase domain found in p190RhoGAP-A and similar proteins

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000019.10 Reference GRCh38.p14 Primary Assembly

    Range
    46860997..47005077
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_024451473.2 → XP_024307241.1  rho GTPase-activating protein 35 isoform X1

    UniProtKB/TrEMBL
    A0A2X0SFV3
    Conserved Domains (4) summary
    smart00441
    Location:431 → 483
    FF; Contains two conserved F residues
    cl02570
    Location:1247 → 1380
    RhoGAP; RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when ...
    pfam16512
    Location:261 → 340
    RhoGAP-FF1; p190-A and -B Rho GAPs FF domain
    cl27030
    Location:158 → 250
    Ras; Ras family

RNA

  1. XR_002958305.2 RNA Sequence

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060943.1 Alternate T2T-CHM13v2.0

    Range
    49686835..49830977
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054320752.1 → XP_054176727.1  rho GTPase-activating protein 35 isoform X1

RNA

  1. XR_008485136.1 RNA Sequence

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_024342.1: Suppressed sequence

    Description
    NM_024342.1: This RefSeq was permanently suppressed because it is a nonsense-mediated decay (NMD) candidate.
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9NRY4.3 GenPept UniProtKB/Swiss-Prot:Q9NRY4

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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