Pde3b phosphodiesterase 3B [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pde3bprovided by RGD
Official Full Name: phosphodiesterase 3Bprovided by RGD
Primary source: RGD:61943
See related: EnsemblRapid:ENSRNOG00000011417 AllianceGenome:RGD:61943
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: RcGIPl; CGI PDE
Summary: Enables 3',5'-cyclic-AMP phosphodiesterase activity; 3',5'-cyclic-GMP phosphodiesterase activity; and phosphoprotein binding activity. Involved in negative regulation of cAMP/PKA signal transduction and negative regulation of insulin secretion involved in cellular response to glucose stimulus. Predicted to be located in Golgi apparatus; endoplasmic reticulum; and membrane. Predicted to be part of guanyl-nucleotide exchange factor complex. Orthologous to human PDE3B (phosphodiesterase 3B). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Liver (RPKM 197.1), Thymus (RPKM 178.1) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q34

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (178041207..178204503)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (168606762..168770078)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (183892841..184078071)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Diosgenin reduces phosphodiesterase 3B (PDE3B) through AMP-activated protein kinase/ mechanistic target of rapamycin (AMPK/mTOR) signaling pathway to ameliorate streptozotocin-induced pancreatic beta-cell apoptosis and dysfunction.
Title: Diosgenin reduces phosphodiesterase 3B (PDE3B) through AMP-activated protein kinase/ mechanistic target of rapamycin (AMPK/mTOR) signaling pathway to ameliorate streptozotocin-induced pancreatic β-cell apoptosis and dysfunction.
C-peptide has the potential to act, conditionally, as an antilipolytic hormone by activating phosphodiesterase-3B.
Title: Proinsulin C-peptide inhibits lipolysis in diabetic rat adipose tissue through phosphodiestrase-3B enzyme.
PI3K but not Akt is an upstream regulator of the PDE3B pathway of leptin signalling in the rat hypothalamus.
Title: Phosphatidylinositol 3-kinase is an upstream regulator of the phosphodiesterase 3B pathway of leptin signalling that may not involve activation of Akt in the rat hypothalamus.
Data demonstrate that AG/UAG attenuation of ISO-induced lipolysis involves PI3Kgamma/AKT and PDE3B.
Title: Acylated and unacylated ghrelin attenuate isoproterenol-induced lipolysis in isolated rat visceral adipocytes through activation of phosphoinositide 3-kinase γ and phosphodiesterase 3B.
PDE3B is activated in response to agents relevant for beta-cell function and activation is linked to increased as well as decreased phosphorylation of the enzyme
Title: Expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets.
PDE3 inhibition reverses leptin-induced stimulation of proopiomelanocortin and neurotensin gene expression; the PDE3B pathway plays an important role in mediating leptin action in the hypothalamus
Title: A role of phosphodiesterase-3B pathway in mediating leptin action on proopiomelanocortin and neurotensin neurons in the hypothalamus.
Increased expression of PDE3B along with decreased cAMP in the exdotoxemic kidneys were associated with reduced renal function, alteration of renal tubular cell morphology, and increased iNOS expression.
Title: The role of phosphodiesterase 3 in endotoxin-induced acute kidney injury.
PDE3B, a key enzyme in insulin signalling, appears to be associated with caveolae in adipocytes and this localization seems to be functionally important.
Title: Plasma membrane cyclic nucleotide phosphodiesterase 3B (PDE3B) is associated with caveolae in primary adipocytes.
plays an important role in the stimulatory action of cAMP on pancreatic beta-cell exocytosis and release of insulin
Title: Important role of phosphodiesterase 3B for the stimulatory action of cAMP on pancreatic beta-cell exocytosis and release of insulin.
data suggest that PDE3B, but not PDE4, contributes to the regulation of insulin-induced glucose uptake, GLUT-4 translocation, and lipogenesis presumably by regulation of a cAMP/Epac signalling mechanisms
Title: Role of PDE3B in insulin-induced glucose uptake, GLUT-4 translocation and lipogenesis in primary rat adipocytes.

Submit: New GeneRIF Correction See all GeneRIFs (12)

General gene information

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Markers

RH134896 (e-PCR)
- UniSTS:218174

AI235078 (e-PCR)
- UniSTS:261207

D1Bda74 (e-PCR)
- UniSTS:239825

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 3',5'-cGMP-inhibited cyclic-nucleotide phosphodiesterase activity	IEA Inferred from Electronic Annotation more info
enables 3',5'-cGMP-stimulated cyclic-nucleotide phosphodiesterase activity	IEA Inferred from Electronic Annotation more info
enables 3',5'-cyclic-AMP phosphodiesterase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3',5'-cyclic-AMP phosphodiesterase activity	IDA Inferred from Direct Assay more info	PubMed
enables 3',5'-cyclic-AMP phosphodiesterase activity	IEA Inferred from Electronic Annotation more info
enables 3',5'-cyclic-AMP phosphodiesterase activity	ISO Inferred from Sequence Orthology more info
enables 3',5'-cyclic-GMP phosphodiesterase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3',5'-cyclic-GMP phosphodiesterase activity	IDA Inferred from Direct Assay more info	PubMed
enables 3',5'-cyclic-nucleotide phosphodiesterase activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphoprotein binding	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase B binding	ISO Inferred from Sequence Orthology more info
enables protein kinase B binding	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in cAMP-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to insulin stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to insulin stimulus	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within endocrine pancreas development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within glucose homeostasis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of angiogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of cAMP/PKA signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of insulin secretion involved in cellular response to glucose stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of lipid catabolic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of lipid catabolic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of insulin secretion	ISO Inferred from Sequence Orthology more info
involved_in regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	ISO Inferred from Sequence Orthology more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
part_of guanyl-nucleotide exchange factor complex	IEA Inferred from Electronic Annotation more info
part_of guanyl-nucleotide exchange factor complex	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B

Names: CGI-PDE B; CGIPDE1; cGMP-inhibited 3',5'-cyclic phosphodiesterase B; cyclic GMP-inhibited phosphodiesterase B; phosphodiesterase 3B, cGMP-inhibited

NP_058925.2

EC 3.1.4.17

XP_038965381.1

EC 3.1.4.17

XP_038965386.1

EC 3.1.4.17

XP_038965388.1

EC 3.1.4.17

XP_038965391.1

EC 3.1.4.17

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_017229.2 → NP_058925.2 cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B

Status: VALIDATED

Source sequence(s)

JAXUCZ010000001

UniProtKB/Swiss-Prot

Q63085

UniProtKB/TrEMBL

F1LQ35

Related

ENSRNOP00000101474.1, ENSRNOT00000157387.1

Conserved Domains (2) summary

pfam00233
Location:718 → 992

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase

pfam11992
Location:145 → 264

DUF3488; Domain of unknown function (DUF3488)

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

178041207..178204503

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039109460.2 → XP_038965388.1 cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B isoform X3

UniProtKB/TrEMBL

A6I8A8
XM_039109453.2 → XP_038965381.1 cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B isoform X1

UniProtKB/TrEMBL

A6I8A7
XM_039109463.2 → XP_038965391.1 cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B isoform X4

UniProtKB/TrEMBL

A6I8A8
XM_039109458.2 → XP_038965386.1 cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B isoform X2

Conserved Domains (1) summary

pfam00233
Location:229 → 492

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase