brk brinker [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: brkprovided by FlyBase
Official Full Name: brinkerprovided by FlyBase
Primary source: FLYBASE:FBgn0024250
Locus tag: Dmel_CG9653
See related: AllianceGenome:FB:FBgn0024250
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: Brk; BRK; CG9653; Dm brk; Dmel\CG9653; ssg-1
Summary: Enables DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Involved in several processes, including cell competition in a multicellular organism; negative regulation of BMP signaling pathway; and posterior Malpighian tubule development. Located in nucleus. Is expressed in several structures, including embryonic/larval midgut primordium; germ layer; head epidermis primordium; imaginal disc; and tracheal primordium. [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 7B1-7B1; 1-20 cM

Exon count:: 1

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	X	NC_004354.4 (7307939..7311132)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	X	NC_004354.3 (7201972..7205165)

Chromosome X - NC_004354.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

brinker levels regulated by a promoter proximal element support germ cell homeostasis.
Title: brinker levels regulated by a promoter proximal element support germ cell homeostasis.
Molecular Dynamics Simulations indicate that Brinker binds to its cognate DNA and folds in approximately 50 mus by an induced fit mechanism, acquiring most of its stable secondary and tertiary structure only after it reaches the final binding site on the DNA.
Title: Complete Coupled Binding-Folding Pathway of the Intrinsically Disordered Transcription Factor Protein Brinker Revealed by Molecular Dynamics Simulations and Markov State Modeling.
we identify key roles for the Zelda and Zerknullt transcription factors in establishing the resulting expression domain, and find widespread binding of insulator proteins to the Mad and Brinker-bound genomic regions. Analysis of embryos lacking the BEAF-32 insulator protein shows reduced transcription of a peak BMP target gene and a reduction in the number of amnioserosa cells, the fate specified by peak BMP signaling.
Title: Regulation of the BMP Signaling-Responsive Transcriptional Network in the Drosophila Embryo.
This work found that mutating the silencer elements in the brk locus results in an expansion of the brk expression domain. However, repression could still be observed in regions of maximal Dpp levels.
Title: Manipulating the sensitivity of signal-induced repression: quantification and consequences of altered brinker gradients.
although the recruitment of Gro is necessary and can be sufficient for Brk to make an almost morphologically wild-type fly, it is insufficient during oogenesis, where Brk must utilize CtBP and 3R to pattern the egg shell appropriately.
Title: Brinker possesses multiple mechanisms for repression because its primary co-repressor, Groucho, may be unavailable in some cell types.
here we investigate the cis-regulatory system controlling brinker (brk) expression in the Drosophila embryo.
Title: Autoregulatory feedback controls sequential action of cis-regulatory modules at the brinker locus.
Brk induces lateral expression indirectly, apparently through repression of a negative regulator.
Title: Inverse regulation of target genes at the brink of the BMP morphogen activity gradient.
Brk represses Myc expression and wing disc growth.
Title: Brk regulates wing disc growth in part via repression of Myc expression.
Bridging Decapentaplegic and Wingless signaling in Drosophila wings through repression of naked cuticle by Brinker
Title: Bridging Decapentaplegic and Wingless signaling in Drosophila wings through repression of naked cuticle by Brinker.
Brinker folding upon DNA-binding might obey induced-fit mechanism
Title: Conformational selection or induced fit for Brinker and DNA recognition.

Submit: New GeneRIF Correction See all GeneRIFs (23)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

fb33b12.x1 (e-PCR)
- UniSTS:190403

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cell competition in a multicellular organism	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc-derived wing morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of BMP signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of BMP signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in posterior Malpighian tubule development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in wing disc morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed