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Ano4 anoctamin 4 [ Mus musculus (house mouse) ]

Gene ID: 320091, updated on 9-Dec-2024

Summary

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Official Symbol
Ano4provided by MGI
Official Full Name
anoctamin 4provided by MGI
Primary source
MGI:MGI:2443344
See related
Ensembl:ENSMUSG00000035189 AllianceGenome:MGI:2443344
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Gm65; Tmem16d; A330096O15Rik
Summary
Enables phospholipid scramblase activity. Acts upstream of or within calcium activated phospholipid scrambling and establishment of localization in cell. Predicted to be active in plasma membrane. Is expressed in dorsal root ganglion and trigeminal ganglion. Orthologous to human ANO4 (anoctamin 4). [provided by Alliance of Genome Resources, Dec 2024]
Expression
Biased expression in ovary adult (RPKM 7.1), frontal lobe adult (RPKM 4.2) and 8 other tissues See more
Orthologs
human all
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Genomic context

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See Ano4 in Genome Data Viewer
Location:
10 C1- C2; 10 44.42 cM
Exon count:
32
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 10 NC_000076.7 (88784794..89181059, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 10 NC_000076.6 (88948932..89345205, complement)

Chromosome 10 - NC_000076.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_27512 Neighboring gene UTP20 small subunit processome component Neighboring gene STARR-seq mESC enhancer starr_27513 Neighboring gene solute carrier family 5 (iodide transporter), member 8 Neighboring gene STARR-seq mESC enhancer starr_27514 Neighboring gene STARR-seq mESC enhancer starr_27515 Neighboring gene predicted gene, 30769 Neighboring gene STARR-seq mESC enhancer starr_27517 Neighboring gene STARR-positive B cell enhancer ABC_E6898 Neighboring gene STARR-seq mESC enhancer starr_27520 Neighboring gene STARR-positive B cell enhancer ABC_E1404 Neighboring gene STARR-seq mESC enhancer starr_27521 Neighboring gene growth arrest-specific 2 like 3 Neighboring gene nuclear receptor subfamily 1, group H, member 4

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Anoctamin 4 channel currents activate glucose-inhibited neurons in the mouse ventromedial hypothalamus during hypoglycemia. Tu L, et al. J Clin Invest, 2023 Jul 17. PMID 37261917, Free PMC Article
  2. Anoctamin-4 is a bona fide Ca(2+)-dependent non-selective cation channel. Reichhart N, et al. Sci Rep, 2019 Feb 19. PMID 30783137, Free PMC Article
  3. ANOs 3-7 in the anoctamin/Tmem16 Cl- channel family are intracellular proteins. Duran C, et al. Am J Physiol Cell Physiol, 2012 Feb 1. PMID 22075693, Free PMC Article
  4. Calcium-dependent phospholipid scramblase activity of TMEM16 protein family members. Suzuki J, et al. J Biol Chem, 2013 May 10. PMID 23532839, Free PMC Article
  5. Wnt signaling regulates ion channel expression to promote smooth muscle and cartilage formation in developing mouse trachea. Russell NX, et al. Am J Physiol Lung Cell Mol Physiol, 2023 Dec 1. PMID 37873566, Free PMC Article

See all (15) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Anoctamin 4 channel currents activate glucose-inhibited neurons in the mouse ventromedial hypothalamus during hypoglycemia.
    Title: Anoctamin 4 channel currents activate glucose-inhibited neurons in the mouse ventromedial hypothalamus during hypoglycemia.
  2. Anoctamin-4 is a bona fide Ca(2+)-dependent non-selective cation channel.
    Title: Anoctamin-4 is a bona fide Ca(2+)-dependent non-selective cation channel.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (5) 

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables chloride channel activity IBA
Inferred from Biological aspect of Ancestor
more info
  
NOT enables intracellularly calcium-gated chloride channel activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables intracellularly calcium-gated chloride channel activity ISO
Inferred from Sequence Orthology
more info
  
enables phospholipid scramblase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein dimerization activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within calcium activated galactosylceramide scrambling IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within calcium activated phosphatidylcholine scrambling IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within calcium activated phosphatidylserine scrambling IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in chloride transmembrane transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in chloride transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in chloride transmembrane transport ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within establishment of localization in cell IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
anoctamin-4
Names
transmembrane protein 16D (eight membrane-spanning domains)

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001277188.1 → NP_001264117.1  anoctamin-4

    See identical proteins and their annotated locations for NP_001264117.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the supported protein.
    Source sequence(s)
    AI463477, AK078364, BC065114, CF535597
    Consensus CDS
    CCDS24114.2
    UniProtKB/Swiss-Prot
    Q8C5H1, Q8CA37
    Related
    ENSMUSP00000138193.2, ENSMUST00000182341.8
    Conserved Domains (2) summary
    pfam04547
    Location:339 → 920
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:111 → 336
    Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

RNA

  1. NR_102339.1 RNA Sequence

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) lacks an internal exon and uses an alternate splice site in the 5' region, compared to variant 1. This variant is represented as non-coding because the use of the expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    AI463477, BC065114
    Related
    ENSMUST00000182462.8

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000076.7 Reference GRCm39 C57BL/6J

    Range
    88784794..89181059 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006513757.5 → XP_006513820.1  anoctamin-4 isoform X3

    Conserved Domains (2) summary
    pfam04547
    Location:503 → 1084
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:275 → 500
    Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

  2. XM_006513756.5 → XP_006513819.1  anoctamin-4 isoform X2

    Conserved Domains (2) summary
    pfam04547
    Location:523 → 1104
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:310 → 520
    Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

  3. XM_006513755.5 → XP_006513818.1  anoctamin-4 isoform X1

    Conserved Domains (2) summary
    pfam04547
    Location:538 → 1119
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:310 → 535
    Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

  4. XM_036155818.1 → XP_036011711.1  anoctamin-4 isoform X6

    Conserved Domains (2) summary
    pfam04547
    Location:168 → 749
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:26 → 165
    Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

  5. XM_011243478.3 → XP_011241780.1  anoctamin-4 isoform X6

    See identical proteins and their annotated locations for XP_011241780.1

    Conserved Domains (2) summary
    pfam04547
    Location:168 → 749
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:26 → 165
    Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

  6. XM_006513759.5 → XP_006513822.1  anoctamin-4 isoform X5

    UniProtKB/TrEMBL
    S4R1L3
    Related
    ENSMUSP00000138268.2, ENSMUST00000182613.8
    Conserved Domains (2) summary
    pfam04547
    Location:304 → 885
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:76 → 301
    Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

  7. XM_030245124.1 → XP_030100984.1  anoctamin-4 isoform X6

    Conserved Domains (2) summary
    pfam04547
    Location:168 → 749
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:26 → 165
    Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

  8. XM_030245123.2 → XP_030100983.1  anoctamin-4 isoform X4

    UniProtKB/Swiss-Prot
    Q8C5H1, Q8CA37
    Conserved Domains (2) summary
    pfam04547
    Location:339 → 920
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:111 → 336
    Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

  9. XM_036155817.1 → XP_036011710.1  anoctamin-4 isoform X5

    UniProtKB/TrEMBL
    S4R1L3
    Related
    ENSMUSP00000138325.2, ENSMUST00000182790.8
    Conserved Domains (2) summary
    pfam04547
    Location:304 → 885
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:76 → 301
    Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

RNA

  1. XR_003948705.2 RNA Sequence

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_178773.4: Suppressed sequence

    Description
    NM_178773.4: This RefSeq was permanently suppressed because the transcript is a nonsense-mediated mRNA decay (NMD) candidate when it uses a supported upstream start codon.
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8C5H1.2 GenPept UniProtKB/Swiss-Prot:Q8C5H1

Gene LinkOut

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