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DNAlig4 DNA ligase 4 [ Drosophila melanogaster (fruit fly) ]

Gene ID: 32322, updated on 9-Dec-2024

Summary

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Official Symbol
DNAlig4provided by FlyBase
Official Full Name
DNA ligase 4provided by FlyBase
Primary source
FLYBASE:FBgn0030506
Locus tag
Dmel_CG12176
See related
AllianceGenome:FB:FBgn0030506
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
CG12176; Dmel\CG12176; lig4; Lig4; LIG4; ligase IV; ligaseIV; ligIV; RE37186
Summary
Predicted to enable ATP binding activity; DNA binding activity; and DNA ligase (ATP) activity. Involved in DNA metabolic process and response to ionizing radiation. Predicted to be part of DNA ligase IV complex and DNA-dependent protein kinase-DNA ligase 4 complex. Is expressed in organism and spermatozoon. Human ortholog(s) of this gene implicated in several diseases, including DNA ligase IV deficiency; colorectal cancer; multiple myeloma; pancreatic cancer; and prostate cancer. Orthologous to human LIG4 (DNA ligase 4). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs
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Genomic context

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See DNAlig4 in Genome Data Viewer
Location:
12B2-12B2; 1-45 cM
Exon count:
4
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) X NC_004354.4 (13664584..13667811, complement)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) X NC_004354.3 (13558617..13561844, complement)

Chromosome X - NC_004354.4Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene NFAT nuclear factor Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. DNA ligase IV mutations confer shorter lifespan and increased sensitivity to nutrient stress in Drosophila melanogaster. Joshi R, et al. J Appl Genet, 2022 Feb. PMID 34817771, Free PMC Article
  2. Secondary structure forming sequences drive SD-MMEJ repair of DNA double-strand breaks. Khodaverdian VY, et al. Nucleic Acids Res, 2017 Dec 15. PMID 29121353, Free PMC Article
  3. Recovery of Alternative End-Joining Repair Products From Drosophila Embryos. Hanscom T, et al. Methods Enzymol, 2018. PMID 29523244
  4. Characterization of sequence contexts that favor alternative end joining at Cas9-induced double-strand breaks. Hanscom T, et al. Nucleic Acids Res, 2022 Jul 22. PMID 35819195, Free PMC Article
  5. Donor DNA Utilization During Gene Targeting with Zinc-Finger Nucleases. Beumer KJ, et al. G3 (Bethesda), 2013 Apr 9. PMID 23550125, Free PMC Article

See all (80) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data show that when both spnA and lig4 were mutated and a circular donor was provided, the frequency of ry mutations was still high and no HR products were recovered.
    Title: Genetic analysis of zinc-finger nuclease-induced gene targeting in Drosophila.
  2. Lig4 is required for repair of DNA double-strand breaks induced by P-element excision.
    Title: Lig4 and rad54 are required for repair of DNA double-strand breaks induced by P-element excision in Drosophila.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables ATP binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA ligase (ATP) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA ligase (ATP) activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA ligase (ATP) activity ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA ligation involved in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA recombination IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in double-strand break repair via nonhomologous end joining IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in double-strand break repair via nonhomologous end joining IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in nucleotide-excision repair, DNA gap filling IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in replication fork processing IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in response to ionizing radiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in telomere maintenance IGI
Inferred from Genetic Interaction
more info
 PubMed 
Component Evidence Code Pubs
part_of DNA ligase IV complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of DNA-dependent protein kinase-DNA ligase 4 complex IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
DNA ligase 4
Names
CG12176-PA
DNA ligase IV
DNAlig4-PA
ligase 4
ligase IV
ligase4
NP_572907.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_004354.4 Reference assembly

    Range
    13664584..13667811 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_132679.3 → NP_572907.1  DNA ligase 4 [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_572907.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q8MS06, Q9VYA5
    Related
    FBpp0073678
    Conserved Domains (5) summary
    cd07968
    Location:469 → 607
    OBF_DNA_ligase_IV; The Oligonucleotide/oligosaccharide binding (OB)-fold domain of ATP-dependent DNA ligase IV is a DNA-binding module that is part of the catalytic core unit
    cd07903
    Location:249 → 466
    Adenylation_DNA_ligase_IV; Adenylation domain of DNA Ligase IV
    smart00292
    Location:668 → 745
    BRCT; breast cancer carboxy-terminal domain
    TIGR00574
    Location:84 → 613
    dnl1; DNA ligase I, ATP-dependent (dnl1)
    pfam04675
    Location:10 → 197
    DNA_ligase_A_N; DNA ligase N terminus
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8MS06 Q8MS06
Q9VYA5 Q9VYA5

Gene LinkOut

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