gce germ cell-expressed bHLH-PAS [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: gceprovided by FlyBase
Official Full Name: germ cell-expressed bHLH-PASprovided by FlyBase
Primary source: FLYBASE:FBgn0261703
Locus tag: Dmel_CG42739
See related: AllianceGenome:FB:FBgn0261703
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG15032; CG42739; CG6211; Dmel\CG42739; Dmgce; Gce; GCE; Gce/Met2
Old locus tag: Dmel_CG15032; Dmel_CG6211
Summary: Enables several functions, including DNA-binding transcription factor binding activity; juvenile hormone binding activity; and nuclear receptor activity. Involved in juvenile hormone mediated signaling pathway; negative regulation of programmed cell death; and positive regulation of transcription by RNA polymerase II. Predicted to be located in cytoplasm. Predicted to be part of aryl hydrocarbon receptor complex. Predicted to be active in nucleus. Is expressed in several structures, including gland; larval neurosecretory cell of pars lateralis; mushroom body; synaptic neuropil domain; and synaptic neuropil layer. [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: all
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Genomic context

Location:: 13B5-13B6; 1-50 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	X	NC_004354.4 (15297766..15319074)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	X	NC_004354.3 (15191799..15213107)

Chromosome X - NC_004354.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The intrinsically disordered region of GCE protein adopts a more fixed structure by interacting with the LBD of the nuclear receptor FTZ-F1.
Title: The intrinsically disordered region of GCE protein adopts a more fixed structure by interacting with the LBD of the nuclear receptor FTZ-F1.
These findings reveal high ligand stereoselectivity of the juvenile hormone receptor Gce, which contrasts with the disparate chemistry of highly potent synthetic juvenile hormone mimics.
Title: Exquisite ligand stereoselectivity of a Drosophila juvenile hormone receptor contrasts with its broad agonist repertoire.
GCE possess specific pattern of localization signals, only partially consistent with presented previously for MET.
Title: Mapping of the Sequences Directing Localization of the Drosophila Germ Cell-Expressed Protein (GCE).
genetic evidence definitively establishes Gce/Met in a Juvenile hormone receptor role, thus resolving a long-standing question in arthropod biology.
Title: Genetic Evidence for Function of the bHLH-PAS Protein Gce/Met As a Juvenile Hormone Receptor.
MF is produced by the larval CA and released into the hemolymph, from where it exerts its anti-metamorphic effects indirectly after conversion to JHB3, as well as acting as a hormone itself through the two JH receptors, Met and Gce.
Title: Methyl farnesoate plays a dual role in regulating Drosophila metamorphosis.
a novel NR box enables MET and GCE to interact JH-dependently with the AF2 of FTZ-F1.
Title: The Drosophila juvenile hormone receptor candidates methoprene-tolerant (MET) and germ cell-expressed (GCE) utilize a conserved LIXXL motif to bind the FTZ-F1 nuclear receptor.
Drosophila Met and Gce redundantly transduce JH action to prevent 20E-induced caspase-dependent PCD during larval molts.
Title: Drosophila Met and Gce are partially redundant in transducing juvenile hormone action.
Data show that gce can substitute in vivo for Met, alleviating preadult but not adult phenotypic characters.
Title: Paralogous genes involved in juvenile hormone action in Drosophila melanogaster.
JH counteracts MET and GCE to prevent caspase-dependent PCD in controlling fat body remodeling and larval-pupal metamorphosis in Drosophila.
Title: Juvenile hormone counteracts the bHLH-PAS transcription factors MET and GCE to prevent caspase-dependent programmed cell death in Drosophila.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA-binding transcription activator activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables bHLH transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables hormone binding	IDA Inferred from Direct Assay more info	PubMed
enables juvenile hormone binding	IDA Inferred from Direct Assay more info	PubMed
enables nuclear receptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in juvenile hormone mediated signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in juvenile hormone mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of programmed cell death	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of DNA-templated transcription	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of aryl hydrocarbon receptor complex	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IC Inferred by Curator more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of transcription regulator complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: germ cell-expressed bHLH-PAS

Names: CG42739-PC; CG42739-PD; CG42739-PE; CG42739-PG; Germ-cell expressed bHLH-PAS; gce-PC; gce-PD; gce-PE; gce-PG; germ cell expressed; germ cell-expressed; germ-cell expressed

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_004354.4 Reference assembly

Range

15297766..15319074

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001272637.2 → NP_001259566.1 germ cell-expressed bHLH-PAS, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001259566.1

Status: REVIEWED

UniProtKB/TrEMBL

G4LU39, Q9VXW7

Conserved Domains (3) summary

cd00083
Location:279 → 325

HLH; Helix-loop-helix domain, found in specific DNA- binding proteins that act as transcription factors; 60-100 amino acids long. A DNA-binding basic region is followed by two alpha-helices separated by a variable loop region; HLH forms homo- and heterodimers, ...

cd00130
Location:352 → 405

PAS; PAS domain; PAS motifs appear in archaea, eubacteria and eukarya. Probably the most surprising identification of a PAS domain was that in EAG-like K+-channels. PAS domains have been found to bind ligands, and to act as sensors for light and oxygen in ...

pfam14598
Location:539 → 646

PAS_11; PAS domain
NM_078605.3 → NP_511160.2 germ cell-expressed bHLH-PAS, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_511160.2

Status: REVIEWED

UniProtKB/TrEMBL

G4LU39, Q9VXW7

Conserved Domains (3) summary

cd00083
Location:279 → 325

HLH; Helix-loop-helix domain, found in specific DNA- binding proteins that act as transcription factors; 60-100 amino acids long. A DNA-binding basic region is followed by two alpha-helices separated by a variable loop region; HLH forms homo- and heterodimers, ...

cd00130
Location:352 → 405

PAS; PAS domain; PAS motifs appear in archaea, eubacteria and eukarya. Probably the most surprising identification of a PAS domain was that in EAG-like K+-channels. PAS domains have been found to bind ligands, and to act as sensors for light and oxygen in ...

pfam14598
Location:539 → 646

PAS_11; PAS domain
NM_001272635.2 → NP_001259564.1 germ cell-expressed bHLH-PAS, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001259564.1

Status: REVIEWED

UniProtKB/TrEMBL

G4LU39, Q9VXW7

Conserved Domains (3) summary

cd00083
Location:279 → 325

HLH; Helix-loop-helix domain, found in specific DNA- binding proteins that act as transcription factors; 60-100 amino acids long. A DNA-binding basic region is followed by two alpha-helices separated by a variable loop region; HLH forms homo- and heterodimers, ...

cd00130
Location:352 → 405

PAS; PAS domain; PAS motifs appear in archaea, eubacteria and eukarya. Probably the most surprising identification of a PAS domain was that in EAG-like K+-channels. PAS domains have been found to bind ligands, and to act as sensors for light and oxygen in ...

pfam14598
Location:539 → 646

PAS_11; PAS domain
NM_001201664.2 → NP_001188593.1 germ cell-expressed bHLH-PAS, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001188593.1

Status: REVIEWED

UniProtKB/TrEMBL

G4LU39, Q9VXW7

Conserved Domains (3) summary

cd00083
Location:279 → 325

HLH; Helix-loop-helix domain, found in specific DNA- binding proteins that act as transcription factors; 60-100 amino acids long. A DNA-binding basic region is followed by two alpha-helices separated by a variable loop region; HLH forms homo- and heterodimers, ...

cd00130
Location:352 → 405

PAS; PAS domain; PAS motifs appear in archaea, eubacteria and eukarya. Probably the most surprising identification of a PAS domain was that in EAG-like K+-channels. PAS domains have been found to bind ligands, and to act as sensors for light and oxygen in ...

pfam14598
Location:539 → 646

PAS_11; PAS domain